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Identification of Functions Affecting Predator-Prey Interactions between Myxococcus xanthus and Bacillus subtilis.
J Bacteriol. 2016 Oct 3;:
Authors: Müller S, Strack SN, Ryan SE, Shawgo M, Walling A, Harris S, Chambers C, Boddicker J, Kirby JR
Soil bacteria engage each other in competitive and cooperative ways to determine their microenvironments. In this study we report the identification of a large number of genes required for Myxococcus xanthus to engage Bacillus subtilis in a predator-prey relationship. We generated and tested over 6000 individual transposon insertion mutants of M. xanthus and found many new factors required to promote efficient predation, including the specialized metabolite myxoprincomide, an ATP-binding cassette (ABC) transporter permease and a CRISPR locus encoding bacterial immunity. We also identified genes known to be involved in predation including those required for production of exopolysaccharides and Type 4 pilus (T4P)-dependent motility, as well as chemosensory and two-component systems. Furthermore, deletion of these genes confirmed their role during predation. Overall, M. xanthus predation appears to be a multifactorial process with multiple determinants enhancing predation capacity.
IMPORTANCE: Soil bacteria engage each other in complex environments and utilize multiple traits to ensure survival. Here we report the identification of multiple traits that enable a common soil organism, Myxococcus xanthus, to prey upon and utilize nutrients from another common soil organism, Bacillus subtilis We mutagenized the predator and carried out a screen to identify genes that were required to either enhance or diminish capacity to consume prey. We identified dozens of genes encoding factors that contribute to the overall repertoire for the predator to successfully engage its prey in the natural environment.
PMID: 27698086 [PubMed - as supplied by publisher]
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