Ophthalmology And Visual Sciences

Nikolai Artemyev, PhD


Professor of Molecular Physiology and Biophysics
Professor of Ophthalmology and Visual Sciences

Contact Information

Office: 5-532  Bowen Science Building
Iowa City, IA 52242
Phone: 319-335-7864

Lab: 5-533  Bowen Science Building
Iowa City, IA 52242
Phone: 319-335-7865

Email: nikolai-artemyev@uiowa.edu


BS, Biotechnology, St. Petersburg Institute of Technology
PhD, St. Petersburg Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

Education/Training Program Affiliations

Department of Molecular Physiology and Biophysics PhD
Interdisciplinary Graduate Program in Molecular and Cellular Biology
Interdisciplinary Graduate Program in Translational Biomedicine
Medical Scientist Training Program

Research Summary

"Heterotrimeric GTP-binding proteins (G-proteins) are used by cells to transduce signals from the extracellular environment through surface receptors to intracellular effector proteins. One of the best model systems for studying mechanisms of G protein signaling is the rod photoreceptor visual transduction cascade. Our laboratory focuses on investigation of specific protein-protein interactions between the photoreceptor – rhodopsin, the rod G protein - transducin, and the effector enzyme - cGMP-phosphodiesterase. We study the modulation of transducin by retinal regulators of G protein signaling (RGS) and other accessory proteins. To understand the activation and turn-off mechanisms of transducin and cGMP-phosphodiesterase upon transduction of a visual signal we employ tools and techniques such as transgenic animal models, site-directed mutagenesis, synthetic peptides, site specific antibodies, cross-linking, and fluorescence spectroscopy. The high levels of sequence homology between different types of G proteins, as well as the structural similarities observed in their crystal structures suggest that numerous G protein signaling systems have similar mechanisms of activation. Our ultimate goal is to understand the mechanisms of regulation of G protein signaling at the molecular level." PubMed link to publications: http://www.ncbi.nlm.nih.gov/pubmed?term=artemyev%20n

Selected Publications

Show All

Manes G, Cheguru P, Majumder A, Bocquet B, Senechal A, Artemyev N, Hamel C, Brabet P.  A Truncated Form of Rod Photoreceptor PDE6 beta-Subunit Causes Autosomal Dominant Congenital Stationary Night Blindness by Interfering with the Inhibitory Activity of the gamma-Subunit.  PLoS One.  2014. 9(4):e95768.

Cheguru P, Zhang Z, Artemyev N.  The GAFa domain of phosphodiesterase-6 contains a rod outer segment localization signal.  J Neurochem.  2014. 129(2):256-63.

Liu X, Kerov V, Haeseleer F, Majumder A, Artemyev N, Baker S, Lee A.  Dysregulation of Ca(v)1.4 channels disrupts the maturation of photoreceptor synaptic ribbons in congenital stationary night blindness type 2.  Channels.  2013. 7(6):514-23.

Sinha S, Majumder A, Belcastro M, Sokolov M, Artemyev N.  Expression and subcellular distribution of UNC119a, a protein partner of transducin alpha subunit in rod photoreceptors.  Cell Signal.  2013. 25(1):341-8.

Majumder A, Gopalakrishna K, Cheguru P, Gakhar L, Artemyev N.  Interaction of Aryl Hydrocarbon Receptor-interacting Protein-like 1 with the Farnesyl Moiety.  J Bio Chem.  2013. 288(29):21320-8.

Majumder A, Pahlberg J, Boyd K, Kerov V, Kolandaivelu S, Ramamurthy V, Sampath A, Artemyev N.  Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells.  Proc Natl Acad Sci USA.  2013. 110(30):12468-73.

Muradov H, Boyd K, Kerov V, Artemyev N.  Atypical retinal degeneration 3 in mice is caused by defective PDE6B pre-mRNA splicing.  Vision Res.  2012. 57:1-8.

Date Last Modified: 06/06/2016 - 13:17:48