Tim Yahr Laboratory

  • Publications

    King JM, Brutinel ED, Marsden AE, Schubot FD, Yahr TL. 2012. Orientation of Pseudomonas aeruginosa ExsA monomers bound to promoter DNA and base-specific contacts with the P(exoT) promoter. J. Bacteriol. 194:2573-85. PMID: 22408167; PMCID: PMC3347218.

    Brutinel ED, King JM, Marsden AE, Yahr TL. 2012. The distal ExsA-binding site in Pseudomonas aeruginosa type III secretion system promoters is the primary determinant for promoter-specific properties. J. Bacteriol. 194:2564-72. PMID: 22408165; PMCID: PMC3347217.

    Zheng Z, Ma D, Yahr TL, Chen L. 2012. The transiently ordered regions in intrinsically disordered ExsE are correlated with structural elements involved in chaperone binding. Biochem. Biophys. Res. Commun. 417:129-34. PMID: 22138394.

    Pezzulo AA, Gutiérrez J, Duschner KS, McConnell KS, Taft PJ, Ernst SE, Yahr TL, Rahmouni K, Klesney-Tait J, Stoltz DA, Zabner J. 2011. Glucose depletion in the airway surface liquid is essential for sterility of the airways. PLoS One. 6:e16166. PMID: 21311590; PMCID: PMC3029092.

    Diaz MR, King JM, Yahr TL. 2011. Intrinsic and extrinsic regulation of Type III secretion gene expression in Pseudomonas aeruginosa. Front. Microbiol. 2:89. PMID: 21833328; PMCID: PMC3153048.

    Jones AK, Fulcher NB, Balzer GJ, Urbanowski ML, Pritchett CL, Schurr MJ, Yahr TL, Wolfgang MC. 2010. Activation of the Pseudomonas aeruginosa AlgU regulon through mucA mutation inhibits cyclic AMP/Vfr signaling. J. Bacteriol. 192:5709-17. PMID: 20817772; PMCID: PMC2953679.

    Fuchs EL, Brutinel ED, Jones AK, Fulcher NB, Urbanowski ML, Yahr TL, Wolfgang MC. 2010. The Pseudomonas aeruginosa Vfr regulator controls global virulence factor expression through cyclic AMP-dependent and -independent mechanisms. J. Bacteriol. 192:3553-64. PMID: 20494996; PMCID: PMC2897347.

    Vakulskas CA, Brutinel ED, Yahr TL. 2010. ExsA recruits RNA polymerase to an extended -10 promoter by contacting region 4.2 of sigma-70. J. Bacteriol. 192:3597-607. PMID: 20453093; PMCID: PMC2897327.

    Fuchs EL, Brutinel ED, Klem ER, Fehr AR, Yahr TL, Wolfgang MC. 2010. In vitro and in vivo characterization of the Pseudomonas aeruginosa cyclic AMP (cAMP) phosphodiesterase CpdA, required for cAMP homeostasis and virulence factor regulation. J. Bacteriol. 192:2779-90. PMID: 20348254; PMCID: PMC2876501.

    Anderson GG, Yahr TL, Lovewell RR, O'Toole GA. 2010. The Pseudomonas aeruginosa magnesium transporter MgtE inhibits transcription of the type III secretion system. Infect. Immun. 78:1239-49. PMID: 20028803; PMCID: PMC2825933.

    Brutinel ED, Vakulskas CA, Yahr TL. 2010. ExsD inhibits expression of the Pseudomonas aeruginosa type III secretion system by disrupting ExsA self-association and DNA binding activity. J. Bacteriol. 192:1479-86. PMID: 20008065; PMCID: PMC2832532.

    Vakulskas CA, Brady KM, Yahr TL. 2009. Mechanism of transcriptional activation by Pseudomonas aeruginosa ExsA. J. Bacteriol. 191:6654-64. PMID: 19717612; PMCID: PMC2795306.

    Brutinel ED, Vakulskas CA, Yahr TL. 2009. Functional domains of ExsA, the transcriptional activator of the Pseudomonas aeruginosa type III secretion system. J. Bacteriol. 191:3811-21. PMID: 19376850; PMCID: PMC2698394.

    Brutinel ED, Vakulskas CA, Brady KM, Yahr TL. 2008. Characterization of ExsA and of ExsA-dependent promoters required for expression of the Pseudomonas aeruginosa type III secretion system. Mol. Microbiol. 68:657-71. PMID: 18373522.

    Brutinel ED, Yahr TL. 2008. Control of gene expression by type III secretory activity. Curr. Opin. Microbiol. 11:128-33. PMID: 18396449; PMCID: PMC2387186.

    Urbanowski ML, Yahr TL. 2008. Limiting too much of a good thing: a negative feedback mechanism prevents unregulated translocation of type III effector proteins. J. Bacteriol. 190:2643-4. PMID: 18083800; PMCID: PMC2293263.

    Urbanowski ML, Brutinel ED, Yahr TL. 2007. Translocation of ExsE into Chinese hamster ovary cells is required for transcriptional induction of the Pseudomonas aeruginosa type III secretion system. Infect. Immun. 75:4432-9. PMID: 17635873; PMCID: PMC1951186.

    Ashare A, Monick MM, Nymon AB, Morrison JM, Noble M, Powers LS, Yarovinsky TO, Yahr TL, Hunninghake GW. 2007. Pseudomonas aeruginosa delays Kupffer cell death via stabilization of the X-chromosome-linked inhibitor of apoptosis protein. J. Immunol. 179:505-13. PMID: 17579071.

    Wu Y, Xu Z, Henderson FC, Ryan AJ, Yahr TL, Mallampalli RK. 2007. Chronic Pseudomonas aeruginosa infection reduces surfactant levels by inhibiting its biosynthesis. Cell Microbiol. 9:1062-72. PMID: 17166234.

    Zheng Z, Chen G, Joshi S, Brutinel ED, Yahr TL, Chen L. 2007. Biochemical characterization of a regulatory cascade controlling transcription of the Pseudomonas aeruginosa type III secretion system. J. Biol. Chem. 282:6136-42. PMID: 17197437.

    Yahr TL, Wolfgang MC. 2006. Transcriptional regulation of the Pseudomonas aeruginosa type III secretion system. Mol. Microbiol. 62:631-40. PMID: 16995895.

    Lykken GL, Chen G, Brutinel ED, Chen L, Yahr TL. 2006. Characterization of ExsC and ExsD self-association and heterocomplex formation. J. Bacteriol. 188:6832-40. PMID: 16980486; PMCID: PMC1595525.

    Yahr TL. 2006. A critical new pathway for toxin secretion? N. Engl. J. Med. 355:1171-2. PMID: 16971725.

    Dasgupta N, Ashare A, Hunninghake GW, Yahr TL. 2006. Transcriptional induction of the Pseudomonas aeruginosa type III secretion system by low Ca2+ and host cell contact proceeds through two distinct signaling pathways. Infect. Immun. 74:3334-41. PMID: 16714561; PMCID: PMC1479281.

    Urbanowski ML, Lykken GL, Yahr TL. 2005. A secreted regulatory protein couples transcription to the secretory activity of the Pseudomonas aeruginosa type III secretion system. Proc. Natl. Acad. Sci. USA. 102:9930-5. PMID: 15985546; PMCID: PMC1175016.

    Yahr TL, Greenberg EP. 2004. The genetic basis for the commitment to chronic versus acute infection in Pseudomonas aeruginosa. Mol. Cell. 16:497-8. PMID: 15546607.

    Dasgupta N, Lykken GL, Wolfgang MC, Yahr TL. 2004. A novel anti-anti-activator mechanism regulates expression of the Pseudomonas aeruginosa type III secretion system. Mol. Microbiol. 53:297-308. PMID: 15225323.

    McCaw ML, Lykken GL, Singh PK, Yahr TL. 2002. ExsD is a negative regulator of the Pseudomonas aeruginosa type III secretion regulon. Mol. Microbiol. 46:1123-33. PMID: 12421316.

    Yahr TL, Wickner WT. 2001. Functional reconstitution of bacterial Tat translocation in vitro. EMBO J. 20:2472-9. PMID: 11350936; PMCID: PMC125449.

    Yahr TL, Wickner WT. 2000. Evaluating the oligomeric state of SecYEG in preprotein translocase. EMBO J. 19:4393-401. PMID: 10944122; PMCID: PMC302025.

    Olson JC, Fraylick JE, McGuffie EM, Dolan KM, Yahr TL, Frank DW, Vincent TS. 1999. Interruption of multiple cellular processes in HT-29 epithelial cells by Pseudomonas aeruginosa exoenzyme S. Infect. Immun. 67:2847-54. PMID: 10338490; PMCID: PMC96591.

    Vallis AJ, Finck-Barbançon V, Yahr TL, Frank DW. 1999. Biological effects of Pseudomonas aeruginosa type III-secreted proteins on CHO cells. Infect. Immun. 67:2040-4. PMID: 10085057; PMCID: PMC96567.

    Sawa T, Yahr TL, Ohara M, Kurahashi K, Gropper MA, Wiener-Kronish JP, Frank DW. 1999. Active and passive immunization with the Pseudomonas V antigen protects against type III intoxication and lung injury. Nat. Med. 5:392-8. PMID: 10202927.

    Vallis AJ, Yahr TL, Barbieri JT, Frank DW. 1999. Regulation of ExoS production and secretion by Pseudomonas aeruginosa in response to tissue culture conditions. Infect. Immun. 67:914-20. PMID: 9916108; PMCID: PMC96404.

    Finck-Barbançon V, Yahr TL, Frank DW. 1998. Identification and characterization of SpcU, a chaperone required for efficient secretion of the ExoU cytotoxin. J. Bacteriol. 180:6224-31. PMID: 9829931; PMCID: PMC107707.

    Yahr TL, Vallis AJ, Hancock MK, Barbieri JT, Frank DW. 1998. ExoY, an adenylate cyclase secreted by the Pseudomonas aeruginosa type III system. Proc. Natl. Acad. Sci. USA. 95:13899-904. PMID: 9811898; PMCID: PMC24955.

    Yahr TL, Mende-Mueller LM, Friese MB, Frank DW. 1997. Identification of type III secreted products of the Pseudomonas aeruginosa exoenzyme S regulon. J. Bacteriol. 179:7165-8. PMID: 9371466; PMCID: PMC179660.

    Liu S, Yahr TL, Frank DW, Barbieri JT. 1997. Biochemical relationships between the 53-kilodalton (Exo53) and 49-kilodalton (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa. J. Bacteriol. 179:1609-13. PMID: 9045820; PMCID: PMC178873.

    Yahr TL, Goranson J, Frank DW. 1996. Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway. Mol. Microbiol. 22:991-1003. PMID: 8971719.

    Yahr TL, Barbieri JT, Frank DW. 1996. Genetic relationship between the 53- and 49-kilodalton forms of exoenzyme S from Pseudomonas aeruginosa. J Bacteriol. 178:1412-9. PMID: 8631719; PMCID: PMC177816.

    Yahr TL, Hovey AK, Kulich SM, Frank DW. 1995. Transcriptional analysis of the Pseudomonas aeruginosa exoenzyme S structural gene. J. Bacteriol. 177:1169-78. PMID: 7868588; PMCID: PMC176720.

    Yahr TL, Frank DW. 1994. Transcriptional organization of the trans-regulatory locus which controls exoenzyme S synthesis in Pseudomonas aeruginosa. J. Bacteriol. 176:3832-38. PMID: 8021164; PMCID: PMC205579.

    Kulich SM, Yahr TL, Mende-Mueller LM, Barbieri JT, Frank DW. 1994. Cloning the structural gene for the 49-kDa form of exoenzyme S (exoS) from Pseudomonas aeruginosa strain 388. J. Biol. Chem. 269:10431-7. PMID: 8144626.