D.P. Mohapatra, Ph.D.

D.P. Mohapatra, Ph.D.
Assistant Professor
Ph.D. (human biology )
University of Erlangen-Nuremberg, Germany, 2003
E-mail: dp-mohapatra@uiowa.edu
Office: 2-252 BSB
Phone: (319) 335-6944
http://web.mac.com/dmohapatra/iWeb/Site%201/HOME.html

Ion Channel Modulations and Regulation of Cellular Plasticity

The intrinsic excitability of neurons as well as cardiac muscles in our body reflects the complex interplay between the inward and outward membrane conductances that underlie the unique electrical activity pattern in each of these cell types, and are governed by the expression, localization and activity of voltage-gated ion channels. In mammalian brain these processes are homeostatically regulated during development and aging, and in response to short- and long-term changes in neuronal activity in the face of sustained alterations in synaptic stimulations, which otherwise could drive the neuronal activity towards extreme excitation or quiescence. Altered expression and/or modulation of specific localization and function of voltage-gated sodium (Nav) and potassium (Kv) channels mainly mediate these homeostatic processes in response to altered neuronal activity, pathological and neuromodulatory stimuli, as well as sensory perceptions.

We are investigating the dynamic modulation of voltage-gated Kv channels in mammalian brain & sensory neurons as well as in cardiac muscles by diverse intracellular signaling pathways resulting from the activation of different types of G-protein coupled receptors (GPCRs), and their contribution to the regulation of cellular plasticity. We combine modern molecular/cell biological, biochemical, mass spectrometry-based proteomic, immunocyto-/histochemical, confocal imaging, and electrophysiological methods in mammalian cell cultures and in animal models to answer these questions. These studies will determine the molecular and cellular mechanisms underlying the intrinsic regulation of neuronal and cardiac muscle excitability and plasticity in several pathological conditions such as epilepsies, Parkinson’s disease, drug abuse, alcoholism, ischemic reperfusion injury, and chronic pain, as well as contributing towards the development of therapeutic interventions for these pathologies.

Representative Publications:

Mohapatra, D.P., Siino, D.F., and Trimmer, J.S.: interdomain cytoplasmic interactions govern the intracellular trafficking, gating and modulation of the Kv2.1 channel. Journal of Neuroscience 28(19):4982-4994, 2008.

Mohapatra, D.P., Park, K.-S., and Trimmer, J.S.: Dynamic regulation of the voltage-gated Kv2.1 potassium channel by multisite phosphorylation. Biochem Society Trans 35(5):1064-1068, 2007.

Park, K.-S.*, Mohapatra, D.P.*, Misonou, H., and Trimmer, J.S.: Graded regulation of the Kv2.1 potassium channel by variable phosphorylation. Science 313(5789):976-979, 2006. *Joint First Author.

Mohapatra, D.P., and Trimmer, J.S.: The Kv2.1 C-terminus can autonomously transfer Kv2.1-like phosphorylation-dependent localization, voltage-dependent gating and muscarinic modulation to diverse Kv channels. J Neurosci 26(2):685-695, 2006.

Mohapatra, D.P., and Nau, C.: Regulation of Ca2+-dependent desensitization in the vanilloid receptor TRPV1 by calcineurin and cAMP-dependent protein kinase. J Biol Chem 280(14):13424-13432, 2005.

Misonou, H., Mohapatra, D.P., Park, E.W., Leung, V., Zhen, D., Misono, K., Anderson, A.E., and Trimmer, J.S.: Regulation of ion channel localization and phosphorylation by neuronal activity. Nature Neurosci 7(7):711-718, 2004.

Click here to see a list of additional publications

Center and Program affiliations:

Biosciences Program

Interdisciplinary Graduate Program in Neuroscience


	D.P. Mohapatra, Ph.D. Assistant Professor Ph.D. (human biology ) University of Erlangen-Nuremberg, Germany, 2003 E-mail: dp-mohapatra@uiowa.edu Office: 2-252 BSB Phone: (319) 335-6944 http://web.mac.com/dmohapatra/iWeb/Site%201/HOME.html
Ion Channel Modulations and Regulation of Cellular Plasticity
The intrinsic excitability of neurons as well as cardiac muscles in our body reflects the complex interplay between the inward and outward membrane conductances that underlie the unique electrical activity pattern in each of these cell types, and are governed by the expression, localization and activity of voltage-gated ion channels. In mammalian brain these processes are homeostatically regulated during development and aging, and in response to short- and long-term changes in neuronal activity in the face of sustained alterations in synaptic stimulations, which otherwise could drive the neuronal activity towards extreme excitation or quiescence. Altered expression and/or modulation of specific localization and function of voltage-gated sodium (Nav) and potassium (Kv) channels mainly mediate these homeostatic processes in response to altered neuronal activity, pathological and neuromodulatory stimuli, as well as sensory perceptions. We are investigating the dynamic modulation of voltage-gated Kv channels in mammalian brain & sensory neurons as well as in cardiac muscles by diverse intracellular signaling pathways resulting from the activation of different types of G-protein coupled receptors (GPCRs), and their contribution to the regulation of cellular plasticity. We combine modern molecular/cell biological, biochemical, mass spectrometry-based proteomic, immunocyto-/histochemical, confocal imaging, and electrophysiological methods in mammalian cell cultures and in animal models to answer these questions. These studies will determine the molecular and cellular mechanisms underlying the intrinsic regulation of neuronal and cardiac muscle excitability and plasticity in several pathological conditions such as epilepsies, Parkinson’s disease, drug abuse, alcoholism, ischemic reperfusion injury, and chronic pain, as well as contributing towards the development of therapeutic interventions for these pathologies. Representative Publications: Mohapatra, D.P., Siino, D.F., and Trimmer, J.S.: interdomain cytoplasmic interactions govern the intracellular trafficking, gating and modulation of the Kv2.1 channel. Journal of Neuroscience 28(19):4982-4994, 2008. Mohapatra, D.P., Park, K.-S., and Trimmer, J.S.: Dynamic regulation of the voltage-gated Kv2.1 potassium channel by multisite phosphorylation. Biochem Society Trans 35(5):1064-1068, 2007. Park, K.-S., Mohapatra, D.P., Misonou, H., and Trimmer, J.S.: Graded regulation of the Kv2.1 potassium channel by variable phosphorylation. Science 313(5789):976-979, 2006. Joint First Author. Mohapatra, D.P., and Trimmer, J.S.: The Kv2.1 C-terminus can autonomously transfer Kv2.1-like phosphorylation-dependent localization, voltage-dependent gating and muscarinic modulation to diverse Kv channels. J Neurosci* 26(2):685-695, 2006. Mohapatra, D.P., and Nau, C.: Regulation of Ca2+-dependent desensitization in the vanilloid receptor TRPV1 by calcineurin and cAMP-dependent protein kinase. J Biol Chem 280(14):13424-13432, 2005. Misonou, H., Mohapatra, D.P., Park, E.W., Leung, V., Zhen, D., Misono, K., Anderson, A.E., and Trimmer, J.S.: Regulation of ion channel localization and phosphorylation by neuronal activity. Nature Neurosci 7(7):711-718, 2004. Click here to see a list of additional publications Center and Program affiliations: Biosciences Program Interdisciplinary Graduate Program in Neuroscience