Specimen:

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 ml light green top tube or 1 light green top microtube for pediatric 
patients

Testing
Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

1 hour (upon receipt in laboratory)

Reference Range:

Males;   0-37 u/l
Females; 0-31 u/l

Pediatric Ranges:
Age             Male/Female U/L
1-3 years            10-50
4-6 years            10-45
7-12 years           10-40
13-18 years          10-35

Comments:

Avoid hemolysis.

Test
Limitations:

Criterion: Recovery within plus or minus 10% of initial value.

Hemolysis interferes due to AST activity from erythrocytes.

Icterus: No significant interference up to an I index of 60 
(approximate conjugated and unconjugated bilirubin concentration: 60 
mg/dl).

Lipemia (Intralipid): No significant interference up to an L index of 
500 (approximate triglycerides concentration: 1000 mg/dl). There is 
poor correlation between turbidity and triglycerides concentration. 
Lipemia may cause absorbance flagging as a result of an absorbance 
increase.

Reference
Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences 
in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474.

Methodology:

Spectrophotometric

Sample
Processing:

Centrifuge at 3000 RPM for 10 minutes.
Aliquot plasma into labeled container and cap.

Sample
Storage:

Refrigerate.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

84450