Specimen

Peripheral Blood, Bone Marrow, or CSF

Collection Medium:

Alternate
Collection Media:

Green top tube (Na Heparin), Lavender top tube (EDTA)

Minimum:

Adult - Peripheral Blood: 10 mL; Bone Marrow:  2-4 mL

Pediatric - Peripheral Blood:  2-5 mL; Bone Marrow:  2-5 mL

CSF - Volume required is cell count dependent.  Provide as much CSF 
specimen as possible in the original CSF collection tube(s).

Delivery Instructions:

Keep at room temperature.  Do not refrigerate.

Testing Schedule:

0800-1630 Monday through Friday.  For additional services, 
contact Clinical Pathology Resident on-call at pager #3404.

Analytic Time:

2 days

Reference Range:

Antibodies routinely included are: CD2, CD3, CD7, CD10, CD13, CD14, 
CD19, CD33, CD34, CD38, CD45, CD46, CD56, CD64, CD117, HLA-DR, Kappa, 
Lambda, Glycophorin A, and Tdt as needed.

Additional antibodies are added as necessary for diagnosis after 
morphologic examination. The pathologist will provide an interpretative 
report.

Comments:

Specimens accepted from Monday 0800 until Friday 1630.

Include pertinent clinical information on the requisition.

Deliver specimen immediately to Immunopathology Lab to ensure leukocyte 
viability.  Do not deliver to specimen control area.

Additional antibodies may be necessary for diagnosis.

Test
Limitations:

Specimens with absolute lymphocyte counts of <100/mm3 will not be
tested.

Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

Technical: 88184(x1), 88185(x18); Professional: 88189

See Additional Information:
Specimens Requiring Immediate Delivery