Specimen:

Peripheral Blood, Bone Marrow, or CSF

Collection Medium:

Alternate
Collection Media:

Green top tube (Na Heparin), Lavender top tube (EDTA)

Minimum:

Adult - Peripheral Blood: 10 mL; Bone Marrow:  2-4 mL

Pediatric - Peripheral Blood:  2-5 mL; Bone Marrow:  2-5 mL

CSF - Volume required is cell count dependent.  Provide as much CSF 
specimen as possible in the original CSF collection tube(s).

Delivery Instructions:

Keep at room temperature.  Do not refrigerate.
Deliver specimen immediately to Immunopathology Lab to ensure leukocyte 
viability.  Do not deliver to specimen control area.

Testing Schedule:

0800-1630 Monday through Friday.  For additional services, 
contact Clinical Pathology Resident on-call at pager #3404.

Analytic Time:

2 days

Reference Range:

Antibodies routinely included are: CD2, CD3, CD7, CD10, CD13, CD14, 
CD19, CD33, CD34, CD38, CD45, CD46, CD56, CD64, CD117, HLA-DR, Kappa, 
Lambda, Glycophorin A, and Tdt as needed.

Additional antibodies are added as necessary for diagnosis after 
morphologic examination. The pathologist will provide an interpretative 
report.

Comments:

Specimens accepted from Monday 0800 until Friday 1630.

Include pertinent clinical information on the requisition.

Additional antibodies may be necessary for diagnosis.

Test
Limitations:

Specimens with absolute lymphocyte counts of <100/mm3 will not be
tested.

Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

Technical: 88184(x1), 88185(x18); Professional: 88189

See Additional Information:
Specimens Requiring Immediate Delivery