Specimen

Peripheral blood

Collection Medium:

Alternate
Collection Media:

Pink top tube (EDTA sprayed)

Minimum:

Adult: 5 mL whole blood (Lavender) or 7 mL whole blood (Pink)

Pediatric: 2 mL whole blood

Delivery Instructions:

Keep at room temperature.  Do not refrigerate.

Testing Schedule:

0800-1630 Monday through Friday.  For additional services, 
contact Clinical Pathology Resident on-call at pager #3404.

Analytic Time:

2 days

Reference Range:

Adult reference ranges for whole blood lysis method by flow cytometry:

T Cells (CD4)     34-62%     Absolute Counts:  298-2045/mm3

CD3 test is run as an internal quality assurance measure as directed by 
CDC guidelines. The results of this QA will not be charged.

Pediatric reference ranges will be provided with the interpretive
report.

Comments:

Specimens with absolute lymphocyte counts of <100/mm3 will not be
tested.

Include pertinent clinical information on the reqisition.  Do not 
deliver to Specimen Control area.

Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

86361

See Additional Information:
Specimens Requiring Immediate Delivery