The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Heat Shock Protein 70 (68 kDa), IgG by Western blot
Order Code: HSP
Order Form: A-1a Miscellaneous Request or Epic Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen:
Serum
Collection Medium:
Red top tube
Minimum:
Preferred Minimum:  0.5 mL serum
Absolute Minimum:  0.2 mL serum
Pediatric Minimum:  0.1 mL serum
Rejection Criteria:
Hemolyzed or lipemic specimens.  Specimens with large clots present or 
bacterial growth.  Specimens collected with anticoagulants or 
preservatives.
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Analytic Time:
within 10 days
Reference Range:
Negative
Interpretive Data:
Western blot assay for HSP70 (68 kDa), IgG antibody may be utilized in 
predicting corticosteroid responsiveness in patients with idiopathic 
rapidly progressive sensorineural hearing loss.  Indeterminate results 
should be repeated using a fresh specimen collected after 10-14 days.  
Other autoimmune diseases may also produce positive results.
Methodology:
Western Blot
CPT Code:
84182

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Updated: 11/19/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.