Specimen

Plasma

Collection Medium:

Minimum:

Preferred minimum: 1 mL plasma from light blue (Na Citrate) top tube
Absolute Adult/Pediatric minimum: 0.5 mL

Rejection Criteria:

Hemolyzed specimens. Serum, EDTA plasma

Analytic Time:

1 week

Reference Range:

By report

Interpretive Data:

Microtiter plates coated with monoclonal anti-ADAMTS13 antibody are 
used to measure ADAMTS13 activity by Fluorescence Resonance Energy 
Transfer (FRET).

ADAMTS13 levels of less than 5% may be associated with either inherited 
(Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic 
purpura (TTP).

A variety of medical conditions may result in a mild to moderate 
deficiency of ADAMTS13 activity. Recent plasma exchange therapy may 
raise the observed ADAMTS13 activity.

The monoclonal anti-ADAMTS13 antibody is directed against the CUB 
domain; therefore, certain forms of ADAMTS13 (with mutations in the CUB 
domains) may not be equivalently measured due to reduced binding to the 
antibody.

Methodology:

Flourescence Resonance Energy Transfer

CPT Code:

85246