Specimen

Serum

Collection Medium:

Alternate
Collection Media:

Light Green top tube (Lithium Heparin)

Minimum:

Adult preferred minimum: 1 mL serum or lithium heparin plasma collected 
from 3 mL whole blood

Adult absolute minimum: 0.6 mL serum or lithium heparin plasma 
collected from 2.0 mL whole blood

Pediatric minimum: 0.6 mL serum or lithium heparin plasma collected 
from 1.8 mL whole blood

Rejection Criteria:

EDTA plasma

Specimen
Instructions:

Specimen should be collected between 6:00 - 10:00 a.m.

Analytic Time:

1 week

Reference Range:

Female - Free Testosterone, pg/mL
1-6 years:        Less than 0.6 pg/mL
7-9 years:        0.6-1.8  pg/mL
10-11 years:      0.1-3.5  pg/mL
12-13 years:      0.9-6.8  pg/mL
14-15 years:      1.2-7.5  pg/mL
16-17 years:      1.2-9.9  pg/mL
18-30 years:      0.8-7.4  pg/mL
31-40 years:      1.3-9.2  pg/mL
41-51 years:      1.1-5.8  pg/mL
Postmenopausal:   0.6-3.8  pg/mL
Tanner Stage I:   Less than 2.2 pg/mL
Tanner Stage II:  0.4-4.5  pg/mL
Tanner Stage III: 1.3-7.5  pg mL
Tanner Stage IV:  1.1-15.5 pg/mL
Tanner Stage V:   0.8-9.2  pg/mL

Male -Free Testosterone, pg/mL
1-6 years:         Less than 0.6 pg/mL
7-9 years:         0.1-0.9   pg/mL
10-11 years:       0.1-6.3   pg/mL
12-13 years:       0.5-98.0  pg/mL
14-15 years:       3-138.0   pg/mL
16-17 years:      38.0-173.0 pg/mL
18 years and older: 47-244   pg/mL
Tanner Stage I:     Less than or equal to 3.7 pg/mL
Tanner Stage II:    0.3-21   pg/mL
Tanner Stage III:   1.0-98.0 pg mL
Tanner Stage IV:     35-169  pg/mL
Tanner Stage V:      41-239  pg/mL

Female Serum Total Testosterone
Premature (26-28 weeks): 5-16 ng/dL
Premature (31-35 weeks): 5-22 ng/dL
Newborn:                20-64 ng/dL
1-7 months: Levels decrease during the first month to less than 10 
ng/dL and remain at this level until puberty.
7-9 years:     Less than 15 ng/dL
10-11 years:   2-42 ng/dL
12-13 years:   6-64 ng/dL
14-15 years:   9-49 ng/dL
16-17 years:   8-63 ng/dL
18-30 years:  11-59 ng/dL
31-40 years:  11-56 ng/dL
41-51 years:   9-55 ng/dL
Postmenopausal:    6-25 ng/dL
Tanner Stage I:    Less than 17 ng/dL
Tanner Stage II:   4-39 ng/dL
Tanner Stage III: 10-60 ng/dL
Tanner Stage IV:   8-63 ng/dL
Tanner Stage V:   10-60 ng/dL


Male Serum Total Testosterone
Premature (26-28 weeks): 59-125 ng/dL
Premature (31-35 weeks): 37-198 ng/dL
Newborn:                 75-400 ng/dL
1-7 months: Levels decrease rapidly the first week to 20-50 ng/dL, and 
then increase to 60-400 ng/dL between 20-60 days.  Levels then decline 
to prepubertal range levels of 3-10 ng/dL by seven months.
7-9 years:   Less than 9 ng/dL
10-11 years: 2-57 ng/dL
12-13 years: 7-747 ng/dL
14-15 years: 33-585 ng/dL
16-17 years: 185-886 ng/dL
18-39 years: 400-1080 ng/dL
40-59 years: 350-890 ng/dL
60 years and older: 350-720 ng/dL
Tanner Stage I:  Less than or equal to 20 ng/dL
Tanner Stage II: 2-149 ng/dL
Tanner Stage III: 7-762 ng/dL
Tanner Stage IV: 164-854 ng/dL
Tanner Stage V: 94-783 ng/dL

Sex Hormone Binding Globulin
Age                      Male                       Female
1-30 days:           13-85  nmol/L              14-60  nmol/L
31-364 days:         70-250 nmol/L              60-215 nmol/L
1-3 years:           50-180 nmol/L              60-190 nmol/L
4-6 years:           45-175 nmol/L              55-170 nmol/L
7-9 years:           28-190 nmol/L              35-170 nmol/L
10-12 years:         23-160 nmol/L              17-155 nmol/L
13-15 years:         13-140 nmol/L              11-120 nmol/L
16-17 years:         10-60  nmol/L              19-145 nmol/L
18 years and older:  11-80  nmol/L              30-135 nmol/L
Tanner Stage I:      26-286 nmol/L              30-173 nmol/L
Tanner Stage II:     22-169 nmol/L              16-127 nmol/L
Tanner Stage III:    13-104 nmol/L              12-98  nmol/L
Tanner Stage IV:     11-60  nmol/L              14-151 nmol/L
Tanner Stage V:      11-71  nmol/L              23-165 nmol/L

Methodology:

Tandem Mass Spectrometry

The concentration of free testosterone is derived from a mathematical 
expression based on the constant for the binding of testosterone to sex 
hormone binding globulin.

CPT Code:

84403 Testosterone, 84270 Sex Hormone Binding Globulin

See also:
Sex Hormone Binding Globulin, Serum or Plasma
Testosterone, Free and Total, Adult Male, Serum
Testosterone, Total, Pediatric, Serum
Testosterone, Total, Plasma