The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK

IGH Gene Clonality by PCR
Order Code: BCELLPCR
Order Form: A-1a Miscellaneous Request or IPR Req 		  Molecular Pathology
6004 BT GH
384-9568 
Specimen
 
Various
Collection Medium:
Lavender top tube (EDTA) 


Minimum:


5 mL blood in a lavender top or yellow top, 1 mL bone marrow aspirate 
in a lavender top or yellow top,  3 cu mm of fresh frozen tissue or 
Formalin-fixed, paraffin embedded tissue, body fluids in a lavender top 
or yellow top or lymph node aspirates in RPMI.


Delivery Instructions:


Submit specimen to laboratory as soon as possible after collection.


Testing Schedule:


Weekly


Analytic Time:


7 working days


Reference Range:


None


Comments:


DNA extracted from blood, bone marrow mononuclear cells or tissue is 
examined for rearrangement of immunoglobin heavy chain genes.


Test
Limitations:


Specimens fixed in B5 fixative are unsuitable for testing.


Methodology:


Multiplex PCR followed by Fluorescence Capillary Electrophoresis


CPT Code:


83890, 83900, 83901(x4), 83909(x3), 83912; if paraffin block or fresh 
tissue is submitted, remove CPT code 83890 and add 83907.




 Alphabetic main page


Updated: 02/19/2007

Note: The information contained in this handbook is  
            for use by personnel of University of Iowa Health Care. 
            No other use is implied or intended.