The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Bcr/Abl T(9;22) Translocation Qualitative by RT-PCR
Order Code: BCRABL
Epic Lab Code: LAB2685
Order Form: A-1a Miscellaneous Request or Epic Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen:
Whole Blood or Bone Marrow
Collection Medium:
Lavender top tube (EDTA)
Alternate
Collection Media:
Pink top tube (EDTA sprayed), Yellow top tube (ACD solution A)
Minimum:
Adult minimum: 5 mL whole blood or 3 mL bone marrow
Pediatric minimum: 1 mL whole blood or 1 mL bone marrow
Rejection Criteria:
Sample MUST be received at reference laboratory within 48 hours of 
collection.
Delivery Instructions:
Deliver to laboratory immediately after collection.
Analytic Time:
2-7 days
Reference Range:
Interpretation:                                                      
Negative - bcr/abl major and minor fusion transcripts are not detected.
Positive [major] - Major bcr/abl fusion transcript is detected, p210.
Positive [minor] - Minor bcr/abl fusion transcript is detected, p190.
Test
Limitations:
Ambient: 1 hour (Samples must be refrigerated within one hour and reach 
the reference laboratory within 48 hours of draw time-due to lability 
of RNA).
Methodology:
Reverse Transcription Polymerase Chain Reaction
CPT Code:
83891, 83902, 83913, 83898(x6), 83894(x3), 83912
 
See Additional Information:
Specimens Requiring Immediate Delivery

Alphabetic main page

Updated: 02/25/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.