The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Reticulocyte Count (Automated)
Order Code: ARET
Epic Lab Code: LAB296
Order Form: A-1a General Lab or Epic Req
  Hematology
6240 RCP
356-3527
Specimen:
Whole Blood
Collection Medium:
Lavender top tube (EDTA)
Minimum:
Full draw; any size lavender top; (or fingerstick)
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Analytic Time:
1 hour (upon receipt in laboratory)
Reference Range:
Age                     Absolute Reticulocyte Range
      1 - 3 Days*            77 - 283 K/mm3
      4 - 30 Days*           14 - 159 K/mm3
     31 - 60 Days*           28 - 201 K/mm3
60 Days - Adult              12 - 130 K/mm3
*Full Term Infant
Comments:
A reticulocyte count can usually be added to any CBC sample <24 hours 
old.
Methodology:
Flow Cytometry
Reticulocyte counts are performed using flow cytometry method.  The 
RNA/DNA in the Reticulocytes is stained and the stained cells are then 
detected and enumerated on the basis of a two-dimensional distribution 
of the forward scattered light and lateral fluorescent light.
CPT Code:
85045

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Updated: 08/19/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.