Specimen

Whole Blood

Minimum:

20 ml; two green tops.  Do not use a needle smaller than 21 gauge.

Delivery Instructions:

Keep at room temperature.  Do not refrigerate.

Reference Range:

Internal control and normal range reported with each sample.  Responses 
to recall antigens assesses the function of memory CD4 lymphocytes, 
response to alloantigen assesses the function of virgin CD4 and CD8 
lymphocytes.

Comments:

Since the assay is run on Tuesday only, the blood must be drawn on 
Tuesday.  Do not centrifuge.  This assay is run using viable 
lymphocytes.  Have floor deliver to Cellular Immunology Lab, VAMC Bldg. 
41, Room 223 or Specimen Control (6240 RCP) within 3 hours of drawing.  
Must be in lab no later than noon.  Test may also be run on Friday, but 
prior arrangements (1-2 day advance notice) are required. Call 
158-7550.

This assay examines the ability of T cells to respond to Tetanus and 
Candida recall antigens and to alloantigen.   For a global assessment 
of T lymphocyte function, order 'Lymphocyte Transformation, 
Spontaneous' and 'Lymphocyte Transformation, Mitogen'.

See also:
Lymphocyte Transformation, Mitogen, Whole Blood
Lymphocyte Transformation, Spontaneous, Whole Blood