The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK

B/T Cell Gene Rearrangement Studies by Southern Blot
Order Code: SBLOT
Order Form: A-1a Miscellaneous Request or IPR Req 		  Molecular Pathology
6004 BT GH
384-9568 
Specimen
 
Various
Collection Medium:
Lavender top tube (EDTA) 


Minimum:


5 ml blood in lavender or yellow top, 1 ml bone marrow aspirate in 
lavendar or yellow top, or 3 cu mm of fresh frozen tissue.  Blood and 
bone marrow must be fresh; separated mononuclear cells may be frozen.


Delivery Instructions:


Submit specimen to laboratory as soon as possible after collection.


Testing Schedule:


Weekly


Analytic Time:


21 days


Comments:


Transport fresh tissue on dry ice.  Blood and bone marrows should be 
transported on ice.  DNA extracted from blood or bone marrow 
mononuclear cells or tissue is examined for rearrangement of 
immunoglobulin heavy chain genes, immunoglobulin kappa chain genes, T 
cell receptor beta chain genes.  Test is run weekly.


Methodology:


Southern Blot


CPT Code:


83890, 83892, 83894, 83896, 83912; if paraffin block or fresh tissue is 
submitted, remove CPT code 83890 and add 83907.




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Updated: 04/11/2007

Note: The information contained in this handbook is  
            for use by personnel of University of Iowa Health Care. 
            No other use is implied or intended.