Specimen

Peripheral Blood

Collection Medium:

Alternate
Collection Media:

Lavender top tube (EDTA)

Minimum:

Adult:  10 mL whole blood in a yellow top tube (ACD solution A)
Pediatric:  2 mL whole blood in a yellow top tube (ACD solution A)

For absolute quantitative results, a CBC with Automated Differential 
must also be ordered.

Delivery Instructions:

Keep at room temperature.  Do not refrigerate.  
Deliver to laboratory immediately after collection.

Testing Schedule:

0800-1630 Monday through Friday.  For additional services, 
contact Clinical Pathology Resident on-call at pager #3404.

Analytic Time:

2 days

Reference Range:

The pathologist will provide an interpretative report.  Antibodies 
routinely included are:  CD3, CD4, CD8, CD14, CD16/56, CD19, CD20 and 
CD45.

Adult reference ranges for peripheral blood by whole blood lysis method 
using flow cytometry:
                                             Absolute Counts
B cells (CD19)                       6-22%   53-726/mm3
T cells (CD3)                       65-85%  569-2804/mm3
T cells (CD4)                       34-62%  298-2045/mm3
T cells (CD8)                       14-42%  122-1386/mm3
T helper/induce (CD4+/CD3+)         30-62%  263-2045/mm3
T cytotoxic/suppressor (CD8+/CD3+)  13-37%  114-1221/mm3
NK cells (CD16+/CD56+/CD3-)         5-31%   44-1023/mm3
NK cells (CD56)                     5-29%   44-957/mm3
CD4/CD8 ratio                       0.9-3.6

Age specific pediatric reference ranges will be provided with the 
interpretive report.

Comments:

Specimens with absolute lymphocyte counts of <100/mm3 will not be
tested.

Test
Limitations:

Specimens with absolute lymphocyte counts of <100/mm3 will not be
tested.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

88184 x1, 88185 x7 Technical, 88187 Professional

See Additional Information:
Specimens Requiring Immediate Delivery