Specimen:

Serum

Collection Medium:

Minimum:

Adult preferred minimum: 1 mL serum
Adult absolute minimum: 0.5 mL serum
Pediatric minimum: 0.15 mL serum

Analytic Time:

4 working days

Reference Range:

< 1.0 ug/mL = Antibody concentration not protective.
Is greater than or equal to 1.0 ug/mL = Antibody to H. influenzae b 
detected. Suggestive of protection.

Comments:

Recommend testing of pre/post vaccination sera when clinically 
indicated.

"Pre" and 30-day "post" Haemophilus influenzae b vaccination samples 
should be submitted together for testing. "Post" sample should be drawn 
30 days after immunization and must be received within 60 days of "pre" 
sample. Please clearly mark samples "pre-vaccine" or "post-vaccine" so 
that samples will be saved and tested simultaneously.

Test
Limitations:

Responder status is determined according to the ratio of 
post-vaccination concentration to pre-vaccination concentration of 
Haemophilus influenza b antibody, IgG as follows:
1. If the post-vaccination concentration is < 3.0 µg/mL, the patient is 
considered to be a nonresponder.
2. If the post-vaccination concentration is greater than or equal
to 3.0 µg/mL, a patient with a ratio of greater than or equal to 4 is a 
good responder, a ratio of 2-4 is a weak responder, and a ratio of < 2 
is considered a nonresponder.

Methodology:

Multi-Analyte Fluorescent Detection

CPT Code:

86317