Specimen

Plasma

Collection Medium:

Alternate
Collection Media:

Call laboratory for additional acceptable specimen collection 
containers.

Minimum:

2 ml whole blood from light green top tube or one 0.6 microtube 
(minimum)

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Reference Range:

Non-allergic Adult:    0-100 IU/ml
Non-allergic Children: 10-20% of adult values

Test
Limitations:

The assay is unaffected by icterus (bilirubin is less than 37 mg/dl), 
hemolysis (Hb is less than 0.1 g/dl), lipemia (triglycerides is less 
than 2200 mg/dl) and biotin is less than 100 ng/ml. (criterion: 
recovery within plus or minus 10% of initial value).

In patients receiving therapy with high biotin doses (i.e. is greater 
than 5 mg/day) no sample should be taken until at least 8 hours after 
the last biotin administration.

No interference was observed from rheumatoid factor up to 6000 U/ml 
(method comparison: Elecsys IgE and a comparison IgE test on 50 
samples).

No high dose hook effect at IgE concentrations of up to 50 000 IU/ml 
(120000 ng/ml).

In vitro tests were performed on 36 commonly used pharmaceuticals. No 
interference with the assay was found.

As with all tests containing monoclonal mouse antibodies, erroneous 
findings may be obtained from samples taken from patients who have been 
treated with monoclonal mouse antibodies or have received them for 
diagnostic purposes.

In rare cases interference due to extremely high titers of antibodies 
to ruthenium can occur.

IgE contains additives which minimize these effects.

Extremely high titers of antibodies to streptavidin can occur in 
isolated cases and cause interference.

For diagnostic purposes, the IgE findings should always be assessed in 
conjunction with the patient's medical history, clinical examination 
and other findings.

Methodology:

Electrochemiluminescence

CPT Code:

82785