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| Hepatitis C Genotype | ||
| Order Code: HCVGENO
Epic Lab Code: LAB2464 Order Form: A-1a Molecular Pathology/Diagnostics or Epic Req |
Molecular Pathology 6004 BT GH 384-9568 |
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Specimen: |
Plasma | ||
Collection Medium: |
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Alternate Collection Media: |
Lavender top tube (EDTA), White top plasma preparation tube (PPT, EDTA) | ||
Minimum: |
3 mL plasma or 6 mL whole blood. if also testing for HIV viral load, collect a second Pink top tube (3 mL plasma or 6 ml whole blood). Both the genotyping and the quantitative assay can be performed from this 2.0 mL. Sample tube must remain sterile. HCV molecular testing cannot be added onto a previously opened vacutainer tube. Testing requires a dedicated collection tube. | ||
Delivery Instructions: |
Deliver to laboratory immediately after collection. | ||
Specimen Instructions: |
Record draw time. All collection tubes need to be processed within six hours of collection. If specimens cannot reach the Microbiology laboratory at UIHC with the six hours, process as follows: Pink or lavender top tubes: Centrifuge for 20 minutes at 800-1600 x g. Aseptically enter the tube and aliquot into sterile RNase free tubes. Transport to the laboratory on ice with 24 hours. White top tube: Centrifuge for 10 minutes at 800-1600 x g. Do not pour off plasma, maintain sterility and transport to the laboratory on ice within 24 hours. | ||
Testing Schedule: |
Once per week | ||
Analytic Time: |
1 week | ||
Methodology: |
Homogeneous Polymerase Chain Reaction (real time PCR) | ||
CPT Code: |
87902 | ||
See Additional Information: Specimens Requiring Immediate Delivery |
Updated: 03/09/2009
Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.