Urine Tests Requiring Preservatives or Special Containers
(Grouped by Tests)

Recommended collection time period is 24 hours, except for pH and bicarbonate. Sodium, potassium, creatinine, and urea nitrogen can be performed on any routine specimen, with or without preservative.

Required Preservative
Test Name
10 g boric acid as a preservative added at start of collection 17-Hydroxycorticosteroids
20 mL 6 N HNO3 (Nitric Acid) Iron
Dark Container, no preservative Porphobilinogen, Qualitative
Dark Glass Container, refrigerate during collection Porphyrins, Quantitative (Fractionated)
Glass container with 10 mL 6N HCl Oxalate
Glass container with 25 mL 6N HCl

Catecholamines, Fractionated
Dopamine
Homovanillic Acid (HVA)
Metanephrines
Vanillylmandelic Acid (VMA)
5-Hydroxyindolacetic Acid (5-HIAA)

Glass container with acid * see below
* Neuroblastoma Screen Acid Quantities

<8 years - no preservative, refrigerate during collection.

>8 years - 15-20 mL 6 N HCL added at start of collection.
Neuroblastoma Screen
Plain container with 10 mL of 6N HCl Histamine
Plastic container with preservative added at start of collection. Contact lab at 6-3527 for preservative vial. Stone Studies
Plastic container, refrigerate during collection

Arsenic
Cadmium
Chromium
Citric Acid
Copper
Lead
Mercury
Methylmalonic Acid, Quantitative

Protect from light AND refrigerate during collection Aminolevulinic Acid, Quantitative
Random container with thin layer of mineral oil

Carbon Dioxide (CO2 Content)
pH, random urine collection only

Refrigerate during entire collection period

Aminolevulinic Acid, Quantitative
Cortisol/Cortisone Urine Free (by LC-MS/MS)
Homocystine
Porphobilinogen, Quantitative

Refrigerate during collection or 10 grams boric acid or 5 mL concentrated HCl are acceptable preservatives Aldosterone