Fraternal Order of Eagles Diabetes Research Center

  • Metabolic Phenotyping Core

    The Metabolic Phenotyping Core provides investigators specialized and non-invasive metabolic assays that are essential in phenotyping mouse and other animal models with diabetes, its complications, obesity, and related metabolic disorders.

    The principal functions of the Core are as follows: 

    • Determination of whole animal energy expenditure using CLAMS (Comprehensive Lab Animal Monitoring System, Columbus Instruments). This is a non-invasive measurement of food intake, energy expenditure, respiratory exchange ratio and physical activity.
    • Measurement of whole body composition with a Bruker MiniSpec.
    • Hyperinsulinemic-euglycemic clamp experiments to assess in vivo insulin action, insulin signaling, and glucose metabolism in awake mice.
    • Hyperglycemic clamp experiments to assess in vivo pancreatic b-cell function (i.e., glucose-induced insulin secretion) and the effect of hyperglycemia on glucose metabolism (i.e., glucose toxicity).
    • Mitochondrial bioenergetics: tissue/cellular/isolated mitochondria oxygen consumption using the XF-24 Extracellular Flux Analyzer; mitochondrial respirometry for tissue (Permeabilized mouse heart and soleus) and isolated mitochondria.
    • Glucose and insulin tolerance tests.

    Consultation with the Technical Director is required before using any service for the 1st time. Please allow 1-2 months’ notice to allow sufficient time to amend ACURF protocols and plan your experiments appropriately. See the ACURF section to download the appropriate ACURF documents and applications.