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The IIHG Genomics Division currently operates the Illumina HiSeq 2000 and HiSeq 2500 genome sequencing platforms. Each instrument has 2 flow cells that each accommodates 8 sequencing lanes. The HiSeq 2500 has the ability to operate in 2 modes, High Output and Rapid mode, while the HiSeq 2000 operates in the High Output mode.
The HiSeq instruments typically yield approximately 175 million reads per lane (range = 150 to 200 million reads). Since most applications require fewer reads, the IIHG Genomics Division can barcode (index) samples to permit the ability to load more than 1 sample per lane. Please contact the Division if you have questions about the depth of coverage needed for your project. Below are the HiSeq instrumentation run metrics.
The Illumina MiSeq is a “benchtop” version of the HiSeq instruments that uses a single lane flow cell capable of yielding 20 to 25 million reads per run. The run time ranges from 24 h (2 x 75 bp PE run) to 65 h (2 x 300 bp PE run) using the v3 sequencing chemistries. Typically 85% of the bases are greater than Q30 for 75 bp reads and are greater than 70% Q30 for 300 bp reads. This instrument is recommend for projects that require fast turn-around but do not require great numbers of reads.
The Genome Sequencing Service operates the Life Technologies Personal Genome Machine (Ion Torrent). This instrument provides the fastest sequencing runs that range from 2 to 7.5 h. This instrument is ideal for de novo sequencing of small genomes or projects that require fewer as listed in the table below.
300H EMRBPhone: (319) 335-6736
Fax: (319) 335-6737
For information about downstream bioinformatics support, contact the IIHG Bioinformatics Division (319) 335-6717.