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The IIHG Genomics Division maintains the following models of real-time or PCR instruments at the designated locations;
The Model 7900HT instruments have interchangeable blocks that permit the running of 96- and 384-well formats, as well as, the TaqMan Low Density Array (TLDA) cards.
Each instrument has a wavelength detection of 500-660 nm allowing for multiple chemistry detection including TaqMan probes and SYBR Green intercalating dyes. Applications include quantitative Reverse Transcriptase PCR (qRT-PCR) and single nucleotide polymorphism (SNP) detection.
Access to the real-time instrumentation is first gained by making a reservation using the on-line calendaring system. The investigator then brings their plate, ready to load on the instrument, along with their completed real-time PCR request form (printed after making your reservation) prior to their reservation time. Results are provided via email or can be transferred to a portable drive provided by the investigator.
Below are links to information which users may want to consider when designing and publishing a real-time PCR experiment.
321 EMRBPhone: (319) 335-7928