Lecia Pewe Epping

Research Assistant III
lecia-pewe@uiowa.edu  

Lab: 319-335-9919
Fax: 319-335-9006 

Training

B.S. (Biology) Saint Ambrose University, Davenport, IA

Research

To determine the localization and distribution of OT-1 CD8 T-cell transgenic eGFP memory cells during Listeria monocytogenes infection in splenic and liver tissues by confocal microscopy.

To determine Influenza virus nucleoprotein prime-boost regimens using dendritic cells or PLGA microspheres to develop strategies for heterosubtypic immunity to Influenza virus. Determine the qualitative differences and distribution of NP147 CD8 memory T-cells after boosting with Vaccinia virus or Listeria monocytogenes that express full-length influenza nucleoprotein in BALB/c mice. Determine the effect of these differences during challenge with PR8 influenza virus.

To determine localization and distribution of Plasmodium yeolii CS280 tg T-cells during Plasmodium yeolii infection by confocal microscopy.

Publications Pubmed

1. Butler NS, Moebius J, Pewe LL, Traore B, Doumbo OK, Tygrett LT, Waldschmidt TJ, Crompton PD, Harty JT. Therapeutic blockade of PD-L1 and LAG-3 rapidly clears established blood-stage Plasmodium infection. Nat Immunol. 2011 Dec 11. doi: 10.1038/ni.2180. [Epub ahead of print]
2. Kashiwada M, Pham NL, Pewe LL, Harty JT, Rothman PB. NFIL₃/E₄BP₄ is a key transcription factor for CD8α+ dendritic cell development. Blood. 2011 Jun 9;117(23):6193-7. Epub 2011 Apr 7.
3. Khanolkar A, Fulton RB, Epping LL, Pham NL, Tifrea D, Varga SM, Harty JT. T cell epitope specificity and pathogenesis of mouse hepatitis virus-1-induced disease in susceptible and resistant hosts. J Immunol. 2010 Jul 15;185(2):1132-41.
4. Pham NL, Pewe LL, Fleenor CJ, Langlois RA, Legge KL, Badovinac VP, Harty JT. Exploiting cross-priming to generate protective CD8 T-cell immunity rapidly. Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12198-203.
5. Khanolkar A, Hartwig SM, Haag BA, Meyerholz DK, Epping LL, Haring JS, Varga SM, Harty JT. Protective and pathologic roles of the immune response to mouse hepatitis virus type 1: implications for severe acute respiratory syndrome. J Virol. 2009 Sep;83(18):9258-72.

Publication acknowledgements of technical assistance:

1. Schmidt NW, Harty JT. Cutting Edge: Attrition of Plasmodium-Specific Memory CD8 T Cells Results in Decreased Protection That Is Rescued by Booster Immunization. J Immunol. 2011 Feb 28. [Epub ahead of print]
2. Butler NS, Schmidt NW, Harty JT. Differential effector pathways regulate memory CD8 T cell immunity against Plasmodium berghei versus P. yoelii sporozoites. J Immunol. 2010 Mar 1;184(5):2528-38. Epub 2010 Jan 22.
3. Zhao DM, Yu S, Zhou X, Haring JS, Held W, Badovinac VP, Harty JT, Xue HH. Constitutive activation of Wnt signaling favors generation of memory CD8 T cells. J Immunol. 2010 Feb 1;184(3):1191-9. Epub 2009 Dec 21.
4. Schmidt NW, Butler NS, Harty JT. CD8 T cell immunity to Plasmodium permits generation of protective antibodies after repeated sporozoite challenge. Vaccine. 2009 Oct 19;27(44):6103-6. Epub 2009 Aug 25.