Biosciences Graduate Program

Budd A. Tucker, PhD

Portrait

Assistant Professor of Ophthalmology and Visual Sciences

Contact Information

Primary Office: 4111  MERF
Iowa City, IA 52242
Primary Office Phone: 319-335-7242

Email: budd-tucker@uiowa.edu
Web: Tucker Stem Cell Laboratory

Education

B.A.H., Biology, Sir Wilfred Grenfell College
PhD, Neuroscience, Memorial University of Newfoundland

Fellowship, Ophthalmology , Harvard Medical School

Education/Training Program Affiliations

Biosciences Graduate Program
Interdisciplinary Graduate Program in Molecular and Cellular Biology
Interdisciplinary Graduate Program in Neuroscience
Interdisciplinary Graduate Program in Translational Biomedicine

Research Summary

During my career I have had extensive experience and published several manuscripts/scientific abstracts relating to stem cell production, culture, isolation, differentiation and transplantation (via both cell injection and polymer scaffold mediated delivery). Two studies of particular importance to this proposal pertain to the use of skin-derived primary cells for the purpose of iPSC generation. The first, published in Plos One, was focused on the use of adult mouse skin derived iPSCs for the purpose of photoreceptor cell production and transplantation induced retinal regeneration. The second, published in PNAS, was focused on production of adult retinal disease specific iPSCs and subsequent human photoreceptors for the purpose of disease modeling and elucidation of disease mechanism. In both studies adult skin derived primary cells were essential for iPSC generation and subsequent experimentation. To date we have generated in excess of 300 molecularly confirmed disease specific human fibroblast and keratinocyte cell lines many of which have been reprogrammed to pluripotency and are now being used for disease modeling, drug screening, and cell transplantation studies.

Selected Publications

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Tucker B, Anfinson K, Mullins R, Stone E, Young M.  Use of a Synthetic Xeno-Free Culture Substrate for Induced Pluripotent Stem Cell Induction and Retinal Differentiation.  Stem Cells Transl Med.  2013 January. 2(1):16-24.
[PubMed]

Menke B, Salem A, Joshi V, Tucker B.  Enhanced Progenitor Cell Integration Following Transplantation of Progenitor Cell/MMP2-Polymer Constructs.  The Association for Research in Vision and Ophthalmology.  2012. 

Burnight E, Kaalberg K, Moses B, Mullins R, Stone E, Tucker B.  LCA Gene Therapy in Somatic-Cell-Derived Induced Pluripotent Stem Cells.  The Association for Research in Vision and Ophthalmology.  2012. 

Checa-Casalengua P, Jiang C, Bravo-Osuna I, Tucker B, Young M, Herrero-Vanrell R.  Preservation of Biological Activity of Glial Cell Line Derived Neurotrophic Factor (GDNF) After Microencapsulation adn Sterilization by Gamma Irradiation.  Int J Pharm.  2012. 436(1-2):545-54.
[PubMed]

Velez G, Weingarden A, Tucker B, Lei H, Kazlauskas A, Young M.  Retinal Pigment Epithelium and Muller Progenitor Cell Interaction Increase Muller Progenitor Cell Expression of PDGFRα and Ability to Induce Proliferative Vitreoretinopathy in a Rabbit Model .  Stem Cell Int .  2012. 2012:106486.
[PubMed]

Stone E, Mullins R, Anfinson K, Scheetz T, Tucker B.  Role of MAK in Human RP.  The Association for Research in Vision and Ophthalmology.  2012. 

Klassen H, Tucker B, Liew C, La Cour M, Wang H.  Stem Cells in Large Animal Models of Retinal and Neurological Disease.  Stem Cells Int.  2012. 2012:460504.
[PubMed]

Tucker B, Mullins R, Anfinson K, Scheetz T, Stone E.  Targeting Human MAK Mutant iPSCs for In Vitro Gene Replacement.  The Association for Research in Vision and Ophthalmology.  2012. 

Tucker B, Sheetz T, Mullins R, Deluca A, Hoffmann J, Johnston R, Jacobson S, Sheffield V, Stone E.  Exome Sequencing and Analysis of Induced Pluripotent Stem Cells Identify the Cilia-Related Gene Male Germ Cell-Associated Kinase (MAK) as a Cause of Retinitis Pigmentosa.  Proc Natl Acad Sci USA.  2011 August 23. 108(34):E569-76.
[PubMed]

Ma J, Kabiel M, Tucker B, Ge J, Young M.  Combining Chondroitinase ABC and Growth Factors Promotes the Integration of Murine Retinal Progenitor Cells Transplanted into Rho (-/-) Mice.  Mol Vis.  2011. 
[PubMed]

Date Last Modified: 06/07/2014 - 21:56:23