Biosciences Graduate Program

George V. Stauffer, PhD

Portrait

Contact Information

Office: 3-352 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
Office Phone: 319-335-7791

Lab: 3-315 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
Phone: 319-335-8866

Email: george-stauffer@uiowa.edu

Education

BS, Pennsylvania State University
MS, Pennsylvania State University
PhD, Pennsylvania State University

Post Doctoral, Stanford University

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Microbiology Graduate Program

Research Summary

Our long-term objective is to understand the basic principles of gene regulation. In Escherichia coli, the gcvB gene encodes a small non-translated RNA that in turn regulates other pathways (e.g., oppA, dppA, cycA and sstT, encoding the oligopeptide and dipeptide periplasmic binding proteins, glycine transport protein and serine transport protein, respectively). The gcvB gene is activated by the GcvA protein in response to glycine and repressed by the GcvA + GcvR proteins in the absence of glycine. Because expression of gcvB follows a pattern of regulation qualitatively similar to the gcvTHP operon, encoding the glycine cleavage enzyme (GCV) complex for generating one-carbon units for cellular methylation reactions, we hypothesize GcvB plays a role in an integrated cellular response to regulate other genes in conjunction with the GCV enzyme system. Using genetics, physiology and biochemistry, we will address two major questions. First, we will determine the secondary structure of GcvB and define regions important for interacting with target mRNAs and regulatory proteins. Second, we will determine the regulatory network controlled by GcvB to gain insight into its role in cell physiology and the molecular mechanisms of control by GcvB.

Center, Program and Institute Affiliations

Center for Biocatalysis and Bioprocessing
Specialized Center for Research in Osteoarthritis

Selected Publications

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Stauffer L, Stauffer G.  The Escherichia coli GcvB sRNA uses genetic redundancy to control cycA expression.  ISRN Microbiol.  2012 May 28. 2012:636273.
[Link]

Stauffer L, Stauffer G.  Antagonistic roles for GcvA and GcvB in hdeAB expression in Escherichia coli.  ISRN Microbiol.  2012 May 16. 2012:697308.
[Link]

Pulvermacher S, Stauffer L, Stauffer G.  Role of the Escherichia coli Hfq protein in GcvB regulation of oppA and dppA mRNAs.  Microbiology.  2009 January. 155(Pt 1):115-23.
[Link]

Pulvermacher S, Stauffer L, Stauffer G.  Role of the sRNA GcvB in regulation of cycA in Escherichia coli.  Microbiology.  2009 January. 155(Pt 1):106-14.
[Link]

Pulvermacher S, Stauffer L, Stauffer G.  The small RNA GcvB regulates sstT mRNA expression in Escherichia coli.  J Bacteriol.  2009 January. 191(1):238-48.
[Link]

Pulvermacher S, Stauffer L, Stauffer G.  The role of the small regulatory RNA GcvB in GcvB/mRNA posttranscriptional regulation of oppA and dppA in Escherichia coli.  FEMS Microbiol Lett.  2008 April. 281(1):42-50.
[Link]

Stauffer L, Stauffer G.  GcvA interacts with both the alpha and sigma subunits of RNA polymerase to activate the Escherichia coli gcvB gene and the gcvTHP operon.  FEMS Microbiol Lett.  2005 January 15. 242(2):333-8.
[Link]

Heil G, Stauffer L, Stauffer G.  Glycine binds the transcriptional accessory protein GcvR to disrupt a GcvA/GcvR interaction and allow GcvA-mediated activation of the Escherichia coli gcvTHP operon.  Microbiology.  2002 July. 148(Pt 7):2203-14.
[Link]

Ghrist A, Heil G, Stauffer G.  GcvR interacts with GcvA to inhibit activation of the Escherichia coli glycine cleavage operon.  Microbiology.  2001 August. 147(Pt 8):2215-21.
[Link]

Wonderling L, Urbanowski M, Stauffer G.  GcvA binding site 1 in the gcvTHP promoter of Escherichia coli is required for GcvA-mediated repression but not for GcvA-mediated activation.  Microbiology.  2000 November. 146 ( Pt 11):2909-18.
[Link]

Date Last Modified: 07/09/2014 - 08:55:28