Biosciences Graduate Program

W. S. Moye-Rowley, PhD

Portrait

Professor of Molecular Physiology and Biophysics

Contact Information

Primary Office: 6-530 BSB
51 Newton Road
Iowa City, IA 52242

Email: scott-moye-rowley@uiowa.edu

Education

BS, Biochemistry, University of Illinois
PhD, Biochemistry, Purdue University

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Molecular Physiology and Biophysics PhD
Interdisciplinary Graduate Program in Genetics
Interdisciplinary Graduate Program in Molecular and Cellular Biology
Interdisciplinary Graduate Program in Neuroscience
Interdisciplinary Graduate Program in Translational Biomedicine
Medical Scientist Training Program

Research Summary

Our laboratory has two primary research interests centered on transcriptional control of gene expression. The first involves the study of the function of a yeast transcriptional regulatory protein. This yeast protein was designated Yap1p by virtue of its homology with a mammalian proto-oncoprotein, c-Jun. c-Jun is a component of a complex DNA binding activity present in animal cells referred to as AP-1. We have constructed yeast strains in which the normal YAP1 gene has been deleted. These yap1 mutant yeast cells were found to be hypersensitive to oxidative stress agents. Additionally, it has been found that transcriptional activity of Yap1p is stimulated by oxidative stress. We are dissecting the molecular events that lead to the activation of Yap1p function using a combination of genetics, molecular biology and biochemistry. The second area of investigation concerns the ability of particular mutant strains of yeast to simultaneously acquire resistance to several cytotoxic drugs with unrelated actions. This phenomenon, called pleiotropic drug resistance (Pdr) in yeast, is related to the multidrug resistance phenotype shown by mammalian tumor cells. Multidrug resistance is a major problem in chemotherapeutic treatment of cancer patients. We will use Pdr in yeast as a model for the mammalian phenotype to gain insight into the molecular events involved in eliciting multidrug resistance.

Selected Publications

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Moye-Rowley W, Paul S, Klutts J.  Differential oxidant tolerance determined by the key transcription factor Yap1 is controlled by levels of the Yap1-binding protein, Ybp1.  2012. 11:1167-1177.

Moye-Rowley W, Gulshan K, Thommandru B.  Proteolytic degradation of the Yap1 transcription factor is regulated by subcellular localization and the E3 ubiquitin ligase Not4.  J Biol Chem. .  2012. 287:26796-26805.

Moye-Rowley W, Morano K, Grant C.  The Response to Heat Shock and Oxidative Stress in Saccharomyces cerevisiae.  2012. 190:1157-1195.

Moye-Rowley W, Gulshan K, Lee S.  Differential oxidant tolerance determined by the key transcription factor Yap1 is controlled by levels of the Yap1-binding protein, Ybp1.  J Biol Chem. .  2011. 286:340721-34081.

Moye-Rowley W, Paul S, Schmidt J.  Regulation of the CgPdr1 transcription factor from the pathogen Candida glabrata.  2011. 10(2):187-197.

Moye-Rowley W, Gulshan K.  Vacuolar import of phosphatidylcholine requires the ATP-binding cassette transporter Ybt1.  2011. 12(9):1257-1268.

Moye-Rowley W, Gulshan K, Shahi P.  Compartment-specific synthesis of phosphatidylethanolamine is required for normal heavy metal resistance.  2010. 21:443-455.

Moye-Rowley W, Shahi P, Gulshan K, Naar A.  Differential Roles of Transcriptional Mediator Subunits in Regulation of Multidrug Resistance Gene Expression in Saccharomyces cerevisiae.  2010. 21:2469-82.

Moye-Rowley W, Johnson S, Hanson P, Manoharlal R, Brice S, Cowart L.  Regulation of yeast nutrient permease endocytosis by ATP-binding cassette transporters and a seven transmembrane protein, RSB1.  J Biol Chem. .  2010. 285(46):35792-802.

Moye-Rowley W, Shahi P.  Coordinate control of lipid composition and drug transport activities is required for normal multidrug resistance in fungi BBA.  2009. 1794:852-859.

Date Last Modified: 08/04/2015 - 09:28:35