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Professor of BiologyProfessor of
Office: 340 BBEIowa City, IA 52242
Email: firstname.lastname@example.orgWeb: More About Dr. Lin - Related Websites and Resources
BS, Medical Technology, National Taiwan UniversityPhD, Mol. Biol. & Biochem., University of Connecticut
Post Doctorate, Cell Biology, Molecular Biology & Biochemistry
Interdisciplinary Graduate Program in GeneticsInterdisciplinary Graduate Program in InformaticsInterdisciplinary Graduate Program in Molecular and Cellular BiologyInterdisciplinary Graduate Program in Neuroscience
Structure and Function of Protein Components of the Cytoskeleton
Animal cells exhibit a wide variety of motile activities, which are essential for the formation and function of tissues. Actin microfilaments have been implicated to the essential force-generating machinery for these motile activities. However, actin filaments alone cannot fully account for these activities. Actin-binding proteins, such as tropomyosin, caldesmon, tropomodulin and myosin Va may play an important role in regulating actin activities In general, our approach is to characterize motile behavior of cells overexpressing various dominant-negative mutant proteins. In addition, we have recently shown that tropomyosin acts as an autoantigen in patiens with ulcerative colitis. We are currently studying the role of tropomyosin in the autoimmune mechanism for this disease. Furthermore, we are also studying the role of tropomyosin and actin filaments in the infection of a parasite Cryptosporidium.
Molecular Mechanisms of Cardiac Morphogenesis
Using rat cardiac troponin T gene, we have defined a minimal promoter conferring the cardiac-specific expression of a reporter gene in transgenic mice. Further characterization of this promoter suggests that novel trans-acting factors are involved in its cardiac-specific expression.
The cloning and characterization of these factors are currently underway. Using differential mRNA displaying, we cloned a novel gene, Xin, from chick and mouse. Incubation of chick embryos with Xin antisense oligonucleotides results in abnormal cardiac morphogenesis. Further characterization shows that Xin participates in the BMP2-Nkx2.5-MEF2C pathway for cardiac development. Xin protein is colocalized with N-cadherin and ß-catenin at the adherens junction of intercalated disc. Mice deficient for Xin have been generated and exhibit cardiac hypertrophy and intercalated disc defects. Studies of this knockout mice leads to the discovery of the second Xin gene in mice. The expressions of both Xin genes are upregulated in mice with pressure overload-induced cardiac hypertrophy. In addition, using large-scale expression (microarray) studies, we have identified several differentially expressed genes for rat heart development.
Date Last Modified: 10/09/2015 -
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