Biosciences Graduate Program

Steven Clegg, PhD

Portrait

Director of Graduate Studies
Professor of Microbiology

Contact Information

Office: 3-334 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
Office Phone: 319-335-7778

Lab: 3-315E Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
Phone: 319-335-7787

Email: steven-clegg@uiowa.edu

Education

BSc, Microbiology, University of Dundee, Scotland
PhD, Pathogenic Bacteriology, University of Dundee, Scotland

Post Doctoral, E. coli ETEC virulence, University of Texas Health Science Center at Houston

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Microbiology Graduate Program
Interdisciplinary Graduate Program in Translational Biomedicine
Medical Scientist Training Program

Research Summary

In both intestinal and extraintestinal infections due to enterobacteria, adhesion of the bacteria to the host tissue is a necessary prerequisite to colonization and infection. Current research projects involve:

1) analysis of the genetic control of fimbrial expression in enteric bacteria,

2) the construction of recombinant plasmids encoding functional fimbriae (pili) and examination of gene products,

3) cloning, by recombinant DNA technology, fimbrial genes from members of the enterobacteriaceae, and

4) examination of recombinant strains for the acquisition of enhanced virulence properties.

Using the techniques of molecular biology the genes coding for fimbrial expression are being studied. Comparison of the structural and regulatory components of this genetic system in different genera of enterobacteria possessing diverse fimbrial antigens is being performed. Many genera of the enteric group of bacteria possess fimbriae which are antigenically distinct, and are expressed only under defined conditions in vitro. A comparison of the genetic elements encoding different types of fimbriae is being performed using gene cloning, DNA sequencing and hybridization, recombinant plasmids and DNA fusion molecules.

Center, Program and Institute Affiliations

George M. O’Brien Kidney Research Center
Institute for Clinical and Translational Science

Selected Publications

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Murphy C, Mortensen M, Krogfelt K, Clegg S.  Role of Klebsiella pneumoniae type 1 and type 3 fimbriae in colonizing silicone tubes implanted into the bladder of mice as a model of catheter-associated urinary tract infections.  Infect Immun.  2013 August. 81(8):3009-17.
[Link]

Rêgo A, Johnson J, Geibel S, Enguita F, Clegg S, Waksman G.  Crystal structure of the MrkD(1P) receptor binding domain of Klebsiella pneumoniae and identification of the human collagen V binding interface.  Mol Microbiol.  2012 November. 86(4):882-93.
[Link]

Kisiela D, Chattopadhyay S, Libby S, Karlinsey J, Fang F, Tchesnokova V, Kramer J, Beskhlebnaya V, Samadpour M, Grzymajlo K, Ugorski M, Lankau E, Mackie R, Clegg S, Sokurenko E.  Evolution of Salmonella enterica virulence via point mutations in the fimbrial adhesin.  PLoS Pathog.  2012 June. 8(6):e1002733.
[Link]

Johnson J, Murphy C, Sippy J, Johnson T, Clegg S.  Type 3 fimbriae and biofilm formation are regulated by the transcriptional regulators MrkHI in Klebsiella pneumoniae.  J Bacteriol.  2011 July. 193(14):3453-60.
[Link]

Johnson J, Clegg S.  Role of MrkJ, a phosphodiesterase, in type 3 fimbrial expression and biofilm formation in Klebsiella pneumoniae.  J Bacteriol.  2010 August. 192(15):3944-50.
[Link]

Nuccio S, Chessa D, Weening E, Raffatellu M, Clegg S, Bäumler A.  SIMPLE approach for isolating mutants expressing fimbriae.  Appl Environ Microbiol.  2007 July. 73(14):4455-62.
[Link]

Boddicker J, Anderson R, Jagnow J, Clegg S.  Signature-tagged mutagenesis of Klebsiella pneumoniae to identify genes that influence biofilm formation on extracellular matrix material.  Infect Immun.  2006 August. 74(8):4590-7.
[Link]

Esteves C, Jones B, Clegg S.  Biofilm formation by Salmonella enterica serovar Typhimurium and Escherichia coli on epithelial cells following mixed inoculations.  Infect Immun.  2005 August. 73(8):5198-203.
[Link]

Jagnow J, Clegg S.  Klebsiella pneumoniae MrkD-mediated biofilm formation on extracellular matrix- and collagen-coated surfaces.  Microbiology.  2003 September. 149(Pt 9):2397-405.
[Link]

Boddicker J, Ledeboer N, Jagnow J, Jones B, Clegg S.  Differential binding to and biofilm formation on, HEp-2 cells by Salmonella enterica serovar Typhimurium is dependent upon allelic variation in the fimH gene of the fim gene cluster.  Mol Microbiol.  2002 September. 45(5):1255-65.
[Link]

Date Last Modified: 06/07/2014 - 21:56:23