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Professor of Molecular Physiology and BiophysicsProfessor of
Ophthalmology and Visual Sciences
Office: 5-532 Bowen Science BuildingIowa City, IA 52242
Lab: 5-533 Bowen Science BuildingIowa City, IA 52242
BS, Biotechnology, St. Petersburg Institute of TechnologyPhD, St. Petersburg Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences
Biosciences Graduate ProgramDepartment of Molecular Physiology and Biophysics PhDInterdisciplinary Graduate Program in Molecular and Cellular BiologyInterdisciplinary Graduate Program in Translational BiomedicineMedical Scientist Training Program
"Heterotrimeric GTP-binding proteins (G-proteins) are used by cells to transduce signals from the extracellular environment through surface receptors to intracellular effector proteins. One of the best model systems for studying mechanisms of G protein signaling is the rod photoreceptor visual transduction cascade. Our laboratory focuses on investigation of specific protein-protein interactions between the photoreceptor – rhodopsin, the rod G protein - transducin, and the effector enzyme - cGMP-phosphodiesterase. We study the modulation of transducin by retinal regulators of G protein signaling (RGS) and other accessory proteins. To understand the activation and turn-off mechanisms of transducin and cGMP-phosphodiesterase upon transduction of a visual signal we employ tools and techniques such as transgenic animal models, site-directed mutagenesis, synthetic peptides, site specific antibodies, cross-linking, and fluorescence spectroscopy.
The high levels of sequence homology between different types of G proteins, as well as the structural similarities observed in their crystal structures suggest that numerous G protein signaling systems have similar mechanisms of activation. Our ultimate goal is to understand the mechanisms of regulation of G protein signaling at the molecular level."
PubMed link to publications:
A Truncated Form of Rod Photoreceptor PDE6 beta-Subunit Causes Autosomal Dominant Congenital Stationary Night Blindness by Interfering with the Inhibitory Activity of the gamma-Subunit.
The GAFa domain of phosphodiesterase-6 contains a rod outer segment localization signal.
Dysregulation of Ca(v)1.4 channels disrupts the maturation of photoreceptor synaptic ribbons in congenital stationary night blindness type 2.
Expression and subcellular distribution of UNC119a, a protein partner of transducin alpha subunit in rod photoreceptors.
Interaction of Aryl Hydrocarbon Receptor-interacting Protein-like 1 with the Farnesyl Moiety.
J Bio Chem.
Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells.
Proc Natl Acad Sci USA.
Atypical retinal degeneration 3 in mice is caused by defective PDE6B pre-mRNA splicing.
Date Last Modified: 08/04/2015 -
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