Internal Medicine

Mark A. Stamnes, PhD

Portrait

Associate Professor of Molecular Physiology and Biophysics
Associate Professor of Internal Medicine

Contact Information

Primary Office: 6-555 BSB
51 Newton Road
Iowa City, IA 52242
Phone: 319-335-7858

Lab: 6-555 BSB
51 Newton Road
Iowa City, IA 52242
Phone: 319-335-7859

Email: mark-stamnes@uiowa.edu

Education

BS, Cellular and Molecular Biology, University of Washington
PhD, Biology, University of California, San Diego

Fellowship, Memorial Sloan-Kettering Cancer Center

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Molecular Physiology and Biophysics PhD
Interdisciplinary Graduate Program in Molecular and Cellular Biology

Research Summary

A central requirement for eukaryotic cell growth and function is the ability to transport and sort proteins. Defects in protein trafficking can lead to debilitating diseases such as cystic fibrosis and Alzheimer's disease. Much of protein transport in a cell occurs via coated transport vesicles. My laboratory's research interests focus on the molecular events involved in the formation of transport vesicles, the selection of cargo molecules into vesicles, and the cellular regulation of protein transport. We use the mammalian Golgi apparatus as a model system for these studies. Transport vesicles can be generated from Golgi membranes in a cell-free system, allowing a biochemical dissection of this process. Projects in the lab include the identification and characterization of novel proteins and phospholipids that play roles in cargo selection of vesicle formation, and an analysis of the role of the cytoskeleton in vesicular transport. A longer term goal of the laboratory is to understand how, once cellular components are properly transported and sorted, they can be assembled into complex cellular structures such as the axons and dendrites of neurons and the microvilli of epithelial cells.

Selected Publications

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Peterson T, Stamnes M.  Analyzing the role of a Golgi transport-vesicle coat protein in PKC-epsilon-dependent signaling.  ASCB Annual meeting.  2011. 

Hehnly H, Xu W, Chen J, Stamnes M.  Cdc42 regulates microtubule-dependent Golgi positioning.  Traffic.  2010. 11:1067-1078.

Stroter R, Stamnes M.  Determining if Coatomer Protein Dependent Localization of Cdc42 and PKCe Contribute to Human Cancers.  Annual Biomedical Research Conference for Minority Students (ABRCMS).  2010. 

Stamnes M, Xu W.  The use of liposomes for studying regulated cytoskeletal dynamics at the membrane.  Methods Mol. Biol..  2010. 606:95-103.

Hehnly H, Longhini K, Chen J, Stamnes M.  Retrograde Shiga toxin trafficking is regulated by ARHGAP21 and Cdc42.  Mol. Biol. Cell.  2009. 20:4303-4312.

Hehnly H, Stamnes M.  Rho GTPases regulat the dynein-dependent motility of Shiga toxin tothe juxtanuclear Golgi apparatus.  The Golgi Meeting: Membrane trafficking in global cellular responses.  2008. 

Hehnly H, Stamnes M.  Regulating cytoskeleton-based vesicle motility.  FEBS lett..  2007. 581:2112-2118.

Xu W, Stamnes M.  Identification of effector proteins that mediate signaling between Golgi vesicles and the cytoskeleton.  ASCB Annual meeting.  2006. 

Pelish H, Peterson J, Salvarezza S, Rodriguez-Boulan E, Chen J, Stamnes M, Macia E, Feng Y, Shair M, Kirchhausen T.  Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro.  Nat. Chem. Biol..  2006. 2:39-46.

Hehnly H, Sheff D, Stamnes M.  Shiga toxin facilitates its retrograde transport by modifying microtubule dynamics.  Mol. Biol. Cell.  2006. 17:4379-4389.

Date Last Modified: 08/04/2015 - 09:28:35