Surgery

Frederick E. Domann, PhD

Portrait

Director, Molecular & Cellular Biology Graduate Program
Co-director, Radiation and Antioxidant Enzyme Core Service
Co-director, Free Radical Cancer Biology Program
Professor of Radiation Oncology
Professor of Pathology, Surgery

Contact Information

Primary Office: B180 ML
Iowa City, IA 52242
Primary Office Phone: 319-335-8018

Email: frederick-domann@uiowa.edu
Web: Free Radical and Radiation Biology Program
Web: Interdisciplinary Graduate Program in Molecular & Cellular Biology
Web: Human Toxicology
Web: Biosciences
Web: Pathology

Education

BS, Chemistry, University of Wisconsin, Platteville, Wisconsin
PhD, Human Cancer Biology, University of Wisconsin, Madision, Wisconsin

Post Doctoral, Department of Radiation Oncology, Arizona Cancer Center, The University of Arizona
Post Doctoral, Department of Pharmacology & Toxicology, , The University of Arizona, Arizona Cancer Center

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Pathology Graduate Program
Free Radical and Radiation Biology Graduate Program
Interdisciplinary Graduate Program in Human Toxicology
Interdisciplinary Graduate Program in Molecular and Cellular Biology
Interdisciplinary Graduate Program in Translational Biomedicine
Medical Scientist Training Program

Research Summary

My laboratory focuses on the transcriptional regulation of cancer related genes including oncogenes and tumor suppressor genes. Specifically, we are studying the molecular mechanisms by which aberrant cytosine methylation of CpG dinucleotides affects gene expression during the development of cancer. We have focused primarily on the tumor suppressor gene SOD2 that encodes the antioxidant enzyme superoxide dismutase. We have determined that site specific DNA methylation of the SOD2 gene promoter can suppress its transcriptional activation. Thus, cytosine methylation of genetic regulatory elements within this important tumor suppressor gene can mediate its inactivation. We are also studying transcription factor AP-2 and its interactions with the SOD2 promoter. Interestingly, these interactions are affected by DNA methylation. We are also assessing chromatin accessibility in the region of the SOD2 promoter in cells that differentially express the gene. Our future research directions will be aimed at elucidating the role of cytosine methylation as a mechanism for inactivation of other genes involved in protection against oxidative damage as well as other classical tumor suppressor genes, and to elucidate the mechanism(s) by which CpG methylation can bring about these changes in gene expression. We have recently become interested in cancer therapy using the human sodium iodide symporter. When expression of this gene is activated, either by gene transfer of epigenetic reactivation, tumor cells acquire the thyroid-like phenotype of iodide concentration. This activity can be exploited as a tool for imaging gene delivery and expression in humans in clinical translational trials of gene therapy; or as a means to deliver therapy itself depnding on which radioisotope of iodine is used.

Center, Program and Institute Affiliations

Cancer and Aging Program
Center for Gene Therapy of Cystic Fibrosis and other Genetic Diseases
Environmental Health Sciences Research Center
Fraternal Order of Eagles Diabetes Research Center
Holden Comprehensive Cancer Center
Institute for Clinical and Translational Science
Iowa Initiative in Human Genetics

All Publications

Vorrink S, Domann F.  Regulatory crosstalk and interference between the xenobiotic and hypoxia sensing pathways at the AhR-ARNT-HIF1α signaling node..  Chemico-Biological Interactions.  2014 July 25. 218C:82-88.
[PubMed]

Vorrink S, Sarsour E, Olivier A, Robertson L, Goswami P, Domann F.  PCB 126 perturbs hypoxia-induced HIF-1α activity and glucose consumption in human HepG2 cells..  Exp Toxicol Pathol. .  2014 June 7. pii: S0940-2993(14):00071-2.
[PubMed]

Hall D, Wu Y, Domann F, Spitz D, Anderson M.  Mitochondrial calcium uniporter activity is dispensable for MDA-MB-231 breast carcinoma cell survival. .  PLoS One.  2014 May 6. 
[PubMed]

Kim Y, Seol D, Mohapatra S, Sunderland J, Schultz M, Domann F, Lim T.  Locally targeted delivery of a micron-size radiation therapy source using temperature-sensitive hydrogel..  International Journal of Radiation Oncology*Biology*Physics.  2014 April 1. 88(5):1142–1147.
[PubMed]

Gross T, Kremens K, Powers L, Brink B, Knutson T, Domann F, Philbert R, Milhem M, Monick M.  Epigenetic silencing of the human NOS2 gene: rethinking the role of nitric oxide in human macrophage inflammatory responses..  The Journal of Immunology.  2014 March 1. 192(5):2326-2338.
[PubMed]

Vorrink S, Severson P, Kulak M, Futscher B, Domann F.  Hypoxia perturbs aryl hydrocarbon receptor signaling and CYP1A1 expression induced by PCB 126 in human skin and liver-derived cell lines..  Toxicology and Applied Pharmacology.  2014 February 1. 274(3):408-16.
[PubMed]

Cyr A, Kulak M, Park J, Bogachek M, Spanheimer P, Woodfield G, White-Baer L, O'Malley Y, Sugg S, Olivier A, Zhang W, Domann F, Weigel R.  TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis..  Oncogene.  2014 January 27. 
[PubMed]

Case A, Domann F.  Absence of manganese superoxide dismutase delays p53-induced tumor formation..  Redox Biology.  2014 January 13. 2:220-223.
[PubMed]

Mezhir J, O'Leary B, Altekruse S, Beardsley R, Henry M, Spitz D, Domann F.  The role of antioxidant enzymes in pancreatic cancer biology and therapy.  AACR Pancreatic Cancer: Innovations in Research and Treatment meeting .  2014. 

Woods C, Venkataraman S, Stearman R, Villegas L, Bowler R, McKinsey T, Ferguson B, Ihida-Stansbury3 K, Geraci M, Stenmark K, Domann F, Nozik-Grayck E.  Regulation of aberrant expression of EC-SOD in IPAH by epigenetic mechanisms.  Annual Meeting of the American Thoracic Society.  2014 January. 

Place T, Nauseef J, Peterson M, Henry M, Mezhir J, Domann F.  Prolyl-4-hydroxylase 3 (PHD3) expression is downregulated during epithelial-to-mesenchymal transition..  PLoS One..  2013 December 18. 8(12):e83021.
[PubMed]

Lal G, Contreras P, Kulak M, Woodfield G, Bair T, Domann F, Weigel R.  Human Melanoma Cells Over-Express Extracellular Matrix 1 (ECM1) Which Is Regulated by TFAP2C..  PLoS One..  2013 September. 8(9):e73953.
[PubMed]

Dholakiya P, Neff T, Smith E, Domann F, Spitz D, Rubenstein L, Bender D, Goodheart M.  Inflammatory Gene Expression and Obesity in Endometrial Carcinoma.  Society of Gynecologic Oncology.  2013 September. 

Gomez-Contreras P, Domann F, Weigel R, Lal G.  Extracellular Matrix 1 (ECM1) knockdown alters cellular characteristics, CD44 expression and other metastasis associated genes in breast cancer cells.  2013 August 31. 

Zhou B, Gibson-Corley K, Herndon M, Sun Y, Gustafson-Wagner E, Teoh-Fitzgerald M, Domann F, Henry M, Stipp C.  Integrin α3β1 Can Function to Promote Spontaneous Metastasis and Lung Colonization of Invasive Breast Carcinoma..  Mol Cancer Res..  2013 August. 
[PubMed]

Sobhakumari A, Schickling B, Love-Homan l, Raeburn A, Fletcher E, Case A, Domann F, Miller, Jr F, Simons A.  NOX4 mediates cytoprotective autophagy induced by the EGFR inhibitor erlotinib in head and neck cancer cells..  Toxicol Appl Pharmacol..  2013 July 31. 
[PubMed]

Jacobus J, Duda C, Coleman M, Martin S, Mapuskar K, Mao G, Smith B, Aykin-Burns N, Guida P, Gius D, Domann F, Kundson C, Spitz D.  Low-Dose Radiation-Induced Enhancement of Thymic Lymphomagenesis in Lck-Bax Mice is Dependent on LET and Gender. .  Radiat Res..  2013 July. 180(2):156-165.
[PubMed]

Cyr A, Hitchler M, Domann F.  Regulation of SOD2 in cancer by histone modifications and CpG methylation: closing the loop between redox biology and epigenetics..  Antioxidants & redox signaling.  2013 May. 18(15):1946-55.
[PubMed]

Case A, Madsen J, Motto D, Meyerholz D, Domann F.  Manganese superoxide dismutase depletion in murine hematopoietic stem cells perturbs iron homeostasis, globin switching, and epigenetic control in erythrocyte precursor cells..  Free radical biology & medicine.  2013 March. 56:17-27.
[PubMed]

Cyr A, Brown K, McCormick M, Coleman M, Case A, Watts G, Futscher B, Spitz D, Domann F.  Maintenance of mitochondrial genomic integrity in the absence of manganese superoxide dismutase in mouse liver hepatocytes..  Redox Biol..  2013 February 5. 1(1):172-7.
[PubMed]

Cyr A, Brown K, McCormick M, Coleman M, Case A, Watts G, Futscher B, Spitz D, Domann F.  Maintenance of mitochondrial genomic integrity in the absence of manganese superoxide dismutase in mouse liver hepatocytes..  Redox Biol..  2013 February 5. 5(1):172-7.
[PubMed]

Khoo N, Hebbar S, Zhao W, Moore S, Domann F, Robbins M.  Differential activation of catalase expression and activity by PPAR agonists: Implications for astrocyte protection in anti-glioma therapy..  Redox Biol..  2013 January 26. 1(1):70-9.
[PubMed]

Khoo N, Hebbar S, Zhao W, Moore S, Domann F, Robbins M.  Differential activation of catalase expression and activity by PPAR agonists: Implications for astrocyte protection in anti-glioma therapy..  Redox Biol..  2013 January 26. 1(1):70-9.
[PubMed]

Domann F.  Aberrant free radical biology is a unifying theme in the etiology and pathogenesis of major human diseases..  International journal of molecular sciences.  2013. 14(4):8491-5.
[PubMed]

Place T, Domann F.  Prolyl-hydroxylase 3: evolving roles for an ancient signaling protein.  Hypoxia.  2013. 
[Link]

Teoh-Fitzgerald M, Fitzgerald M, Zhong W, Askeland R, Domann F.  Epigenetic reprogramming governs EcSOD expression during human mammary epithelial cell differentiation, tumorigenesis and metastasis..  Oncogene.  2013 January. 
[PubMed]

Hitchler M, Domann F.  Redox regulation of the epigenetic landscape in cancer: a role for metabolic reprogramming in remodeling the epigenome..  Free radical biology & medicine.  2012 December. 53(11):2178-87.
[PubMed]

Case A, Domann F.  Manganese superoxide dismutase is dispensable for post-natal development and lactation in the murine mammary gland..  Free radical research.  2012 November. 46(11):1361-8.
[PubMed]

Kulak M, Cyr A, Woodfield G, Bogachek M, Spanheimer P, Li T, Price D, Domann F, Weigel R.  Transcriptional regulation of the GPX1 gene by TFAP2C and aberrant CpG methylation in human breast cancer..  Oncogene.  2012 September. 
[PubMed]

Duangmano S, Sae-Lim P, Suksamrarn A, Patmasiriwat P, Domann F.  Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2/M Cell Cycle Arrest..  Journal of oncology.  2012. 2012:601682.
[PubMed]

Duangmano S, Sae-Lim P, Suksamrarn A, Domann F, Patmasiriwat P.  Cucurbitacin B inhibits human breast cancer cell proliferation through disruption of microtubule polymerization and nucleophosmin/B23 translocation..  BMC complementary and alternative medicine.  2012. 12:185.
[PubMed]

Case A, Mezhir J, O'Leary B, Hrabe J, Domann F.  Rational design of a secreted enzymatically inactive mutant of extracellular superoxide dismutase..  Redox report : communications in free radical research.  2012. 17(6):239-45.
[PubMed]

Teoh-Fitzgerald M, Fitzgerald M, Jensen T, Futscher B, Domann F.  Genetic and epigenetic inactivation of extracellular superoxide dismutase promotes an invasive phenotype in human lung cancer by disrupting ECM homeostasis..  Molecular cancer research : MCR.  2012 January. 10(1):40-51.
[PubMed]

Owens K, Aykin-Burns N, Dayal D, Coleman M, Domann F, Spitz D.  Genomic instability induced by mutant succinate dehydrogenase subunit D (SDHD) is mediated by O2(-•) and H2O2..  Free radical biology & medicine.  2012 January. 52(1):160-6.
[PubMed]

Yue W, Clark J, Fernando A, Domann F, Hansen M.  Contribution of persistent C-Jun N-terminal kinase activity to the survival of human vestibular schwannoma cells by suppression of accumulation of mitochondrial superoxides..  Neuro-oncology.  2011 September. 13(9):961-73.
[PubMed]

Cyr A, Domann F.  The redox basis of epigenetic modifications: from mechanisms to functional consequences..  Antioxidants & redox signaling.  2011 July. 15(2):551-89.
[PubMed]

Tang X, Sun Z, Runne C, Madsen J, Domann F, Henry M, Lin F, Chen S.  A critical role of Gbetagamma in tumorigenesis and metastasis of breast cancer..  The Journal of biological chemistry.  2011 April. 286(15):13244-54.
[PubMed]

Case A, McGill J, Tygrett L, Shirasawa T, Spitz D, Waldschmidt T, Legge K, Domann F.  Elevated mitochondrial superoxide disrupts normal T cell development, impairing adaptive immune responses to an influenza challenge..  Free radical biology & medicine.  2011 February. 50(3):448-58.
[PubMed]

Aykin-Burns N, Slane B, Liu A, Owens K, O'Malley M, Smith B, Domann F, Spitz D.  Sensitivity to low-dose/low-LET ionizing radiation in mammalian cells harboring mutations in succinate dehydrogenase subunit C is governed by mitochondria-derived reactive oxygen species..  Radiation research.  2011 February. 175(2):150-8.
[PubMed]

Place T, Fitzgerald M, Venkataraman S, Vorrink S, Case A, Teoh M, Domann F.  Aberrant promoter CpG methylation is a mechanism for impaired PHD3 expression in a diverse set of malignant cells..  PloS one.  2011. 6(1):e14617.
[PubMed]

Fitzgerald M, Gourronc F, Teoh M, Provenzano M, Case A, Martin J, Domann F.  Human Chondrosarcoma Cells Acquire an Epithelial-Like Gene Expression Pattern via an Epigenetic Switch: Evidence for Mesenchymal-Epithelial Transition during Sarcomagenesis..  Sarcoma.  2011. 2011:598218.
[PubMed]

Woodfield G, Chen Y, Bair T, Domann F, Weigel R.  Identification of primary gene targets of TFAP2C in hormone responsive breast carcinoma cells..  Genes, chromosomes & cancer.  2010 October. 49(10):948-62.
[PubMed]

Drake J, Barnes J, Madsen J, Domann F, Stipp C, Henry M.  ZEB1 coordinately regulates laminin-332 and {beta}4 integrin expression altering the invasive phenotype of prostate cancer cells..  The Journal of biological chemistry.  2010 October. 285(44):33940-8.
[PubMed]

Fitzgerald M, Madsen J, Coleman M, Teoh M, Westphal S, Spitz D, Radi R, Domann F.  Transgenic biosynthesis of trypanothione protects Escherichia coli from radiation-induced toxicity..  Radiation research.  2010 September. 174(3):290-6.
[PubMed]

Dayal D, Martin S, Owens K, Aykin-Burns N, Zhu Y, Boominathan A, Pain D, Limoli C, Goswami P, Domann F, Spitz D.  Mitochondrial complex II dysfunction can contribute significantly to genomic instability after exposure to ionizing radiation..  Radiation research.  2009 December. 172(6):737-45.
[PubMed]

Sinthupibulyakit C, Grimes K, Domann F, Xu Y, Fang F, Ittarat W, St Clair D, St Clair W.  p53 is an important factor for the radiosensitization effect of 2-deoxy-D-glucose..  International journal of oncology.  2009 September. 35(3):609-15.
[PubMed]

Teoh M, Fitzgerald M, Oberley L, Domann F.  Overexpression of extracellular superoxide dismutase attenuates heparanase expression and inhibits breast carcinoma cell growth and invasion..  Cancer research.  2009 August. 69(15):6355-63.
[PubMed]

Hitchler M, Domann F.  Metabolic defects provide a spark for the epigenetic switch in cancer..  Free radical biology & medicine.  2009 July. 47(2):115-27.
[PubMed]

Woodfield G, Hitchler M, Chen Y, Domann F, Weigel R.  Interaction of TFAP2C with the estrogen receptor-alpha promoter is controlled by chromatin structure..  Clinical cancer research : an official journal of the American Association for Cancer Research.  2009 June. 15(11):3672-9.
[PubMed]

Case A, Domann F.  Hypoxia-Induced Gene Regulation through Hypoxia Inducible Factor-1α.  Wiley Online Library- Wiley-VCH Verlag GmbH & Co. KGaA.  2009 May 28. 
[Link]

Yu L, Hitchler M, Sun W, Sarsour E, Goswami P, Klingelhutz A, Domann F.  AP-2alpha Inhibits c-MYC Induced Oxidative Stress and Apoptosis in HaCaT Human Keratinocytes..  Journal of oncology.  2009. 2009:780874.
[PubMed]

Zhao R, Xiang N, Domann F, Zhong W.  Effects of selenite and genistein on G2/M cell cycle arrest and apoptosis in human prostate cancer cells..  Nutrition and cancer.  2009. 61(3):397-407.
[PubMed]

Hitchler M, Oberley L, Domann F.  Epigenetic silencing of SOD2 by histone modifications in human breast cancer cells..  Free radical biology & medicine.  2008 December. 45(11):1573-80.
[PubMed]

Rahimov F, Marazita M, Visel A, Cooper M, Hitchler M, Rubini M, Domann F, Govil M, Christensen K, Bille C, Melbye M, Jugessur A, Lie R, Wilcox A, Fitzpatrick D, Green E, Mossey P, Little J, Steegers-Theunissen R, Pennacchio L, Schutte B, Murray J.  Disruption of an AP-2alpha binding site in an IRF6 enhancer is associated with cleft lip..  Nature genetics.  2008 November. 40(11):1341-7.
[PubMed]

Lal G, Padmanabha L, Provenzano M, Fitzgerald M, Weydert J, Domann F.  Regulation of 14-3-3sigma expression in human thyroid carcinoma is epigenetically regulated by aberrant cytosine methylation..  Cancer letters.  2008 August. 267(1):165-74.
[PubMed]

Dong L, Low P, Dyason J, Wang X, Prochazka L, Witting P, Freeman R, Swettenham E, Valis K, Liu J, Zobalova R, Turanek J, Spitz D, Domann F, Scheffler I, Ralph S, Neuzil J.  Alpha-tocopheryl succinate induces apoptosis by targeting ubiquinone-binding sites in mitochondrial respiratory complex II..  Oncogene.  2008 July. 27(31):4324-35.
[PubMed]

Ahmad I, Abdalla M, Aykin-Burns N, Simons A, Oberley L, Domann F, Spitz D.  2-Deoxyglucose combined with wild-type p53 overexpression enhances cytotoxicity in human prostate cancer cells via oxidative stress..  Free radical biology & medicine.  2008 March. 44(5):826-34.
[PubMed]

Watts G, Futscher B, Holtan N, Degeest K, Domann F, Rose S.  DNA methylation changes in ovarian cancer are cumulative with disease progression and identify tumor stage..  BMC medical genomics.  2008. 1:47.
[PubMed]

Provenzano M, Domann F.  A role for epigenetics in hearing: Establishment and maintenance of auditory specific gene expression patterns..  Hearing research.  2007 November. 233(2-Jan):13-Jan.
[PubMed]

Provenzano M, Fitzgerald M, Krager K, Domann F.  Increased iodine uptake in thyroid carcinoma after treatment with sodium butyrate and decitabine (5-Aza-dC)..  Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.  2007 November. 137(5):722-8.
[PubMed]

Hitchler M, Domann F.  An epigenetic perspective on the free radical theory of development..  Free radical biology & medicine.  2007 October. 43(7):1023-36.
[PubMed]

Provenzano M, Yu L, Hitchler M, Fitzgerald M, Robinson R, Wayne S, Ver Meer M, Domann F.  AP-2 participates in the transcriptional control of the amyloid precursor protein (APP) gene in oral squamous cell carcinoma..  Experimental and molecular pathology.  2007 October. 83(2):277-82.
[PubMed]

Bock J, Menon S, Goswami P, Sinclair L, Bedford N, Domann F, Trask D.  Relative non-steroidal anti-inflammatory drug (NSAID) antiproliferative activity is mediated through p21-induced G1 arrest and E2F inhibition..  Molecular carcinogenesis.  2007 October. 46(10):857-64.
[PubMed]

Ianzini F, Domann F, Kosmacek E, Phillips S, Mackey M.  Human glioblastoma U87MG cells transduced with a dominant negative p53 (TP53) adenovirus construct undergo radiation-induced mitotic catastrophe..  Radiation research.  2007 August. 168(2):183-92.
[PubMed]

Menon S, Sarsour E, Kalen A, Venkataraman S, Hitchler M, Domann F, Oberley L, Goswami P.  Superoxide signaling mediates N-acetyl-L-cysteine-induced G1 arrest: regulatory role of cyclin D1 and manganese superoxide dismutase..  Cancer research.  2007 July. 67(13):6392-9.
[PubMed]

Bock J, Menon S, Sinclair L, Bedford N, Goswami P, Domann F, Trask D.  Celecoxib toxicity is cell cycle phase specific..  Cancer research.  2007 April. 67(8):3801-8.
[PubMed]

Tompkins V, Hagen J, Frazier A, Lushnikova T, Fitzgerald M, di Tommaso A, Ladeveze V, Domann F, Eischen C, Quelle D.  A novel nuclear interactor of ARF and MDM2 (NIAM) that maintains chromosomal stability..  The Journal of biological chemistry.  2007 January. 282(2):1322-33.
[PubMed]

Zhao R, Domann F, Zhong W.  Apoptosis induced by selenomethionine and methioninase is superoxide mediated and p53 dependent in human prostate cancer cells..  Molecular cancer therapeutics.  2006 December. 5(12):3275-84.
[PubMed]

Hitchler M, Wikainapakul K, Yu L, Powers K, Attatippaholkun W, Domann F.  Epigenetic regulation of manganese superoxide dismutase expression in human breast cancer cells..  Epigenetics : official journal of the DNA Methylation Society.  2006 September. 1(4):163-71.
[PubMed]

Li H, Watts G, Oshiro M, Futscher B, Domann F.  AP-2alpha and AP-2gamma are transcriptional targets of p53 in human breast carcinoma cells.  Oncogene.  2006. 25(39):5405-5415.
[PubMed]

Li H, Goswami P, Domann F.  AP-2gamma induces p21 expression, arrests cell cycle, and inhibits the tumor growth of human carcinoma cells..  Neoplasia.  2006. 8(7):568-577.
[PubMed]

Zhao R, Domann F, Zhong W.  Apoptosis induced by selenomethionine and methioninase is superoxide mediated and p53 dependent in human prostate cancer cells.  Mol Cancer Ther.  2006. 12:3275-3284.
[PubMed]

Berger K, Barriga F, Lace M, Turek L, Zamba G, Domann F, Lee J, Klingelhutz A.  Cervical keratinocytes containing stably replicating extrachromosomal HPV-16 are refractory to transformation by oncogenic H-Ras.  Virology.  2006. 356(1-2):68-78.
[PubMed]

Niu G, Anderson R, Madsen M, Graham M, Oberley L, Domann F.  Dual-expressing adenoviral vectors encoding the sodium iodide symporter for use in noninvasive radiological imaging of therapeutic gene transfer.  Nucl Med Biol.  2006. 33(3):391-398.
[PubMed]

Hitchler M, Wikainapakul K, Yu L, Powers K, Attatippaholkun W, Domann F.  Epigenetic regulation of manganese superoxide dismutase expression in human breast cancer cells.  Epigenetics.  2006. 1(4):163-171.
[PubMed]

Rose S, Fitzgerald M, White N, Hitchler M, Futscher B, De Geest K, Domann F.  Epigenetic regulation of maspin expression in human ovarian carcinoma cells.  Gynecologic Oncology.  2006. 102(2):319-324.
[PubMed]

Zhao R, Xiang N, Domann F, Zhong W.  Expression of p53 enhances selenite-induced superoxide production and apoptosis in human prostate cancer cells.  Cancer Res.  2006. 66(4):2296-304.
[PubMed]

Yu L, Venkataraman S, Coleman M, Spitz D, Wertz P, Domann F.  Glutathione peroxidase-1 inhibits UVA-induced AP-2alpha expression in human keratinocytes.  Biochem Biophys Res Commun.  2006. 351(4):1066-1071.
[PubMed]

Luo J, Spitz D, Li L, Sun W, Buettner G, Oberley L, Domann F.  Inactivation of Cellular Antioxidants by Photodynamic Action Enhances Radiation and Adriamycin Induced Cytotoxicity in Human Melanoma cells.  Antioxid Redox Signal.  2006. 8(7-8):1307-1314.
[PubMed]

Luo J, Li L, Zhang Y, Spitz D, Buettner G, Oberley L, Domann F.  Inactivation of primary antioxidant enzymes in mouse keratinocytes by photodynamically generated singlet oxygen.  Antioxid Redox Signal.  2006. 8(7-8):1307-1314.
[PubMed]

Andringa K, Coleman M, Aykin-Burns N, Hitchler M, Walsh S, Domann F, Spitz D.  Inhibition of glutamate cysteine ligase activity sensitizes human breast cancer cells to the toxicity of 2-deoxy-D-glucose..  Cancer Res.  2006. 66(3):1605-1610.
[PubMed]

Moussavi-Harami F, Mollano A, Martin J, Ayoob A, Domann F, Gitelis S, Buckwalter J.  Intrinsic radiation resistance in human chondrosarcoma cells.  Biochemical and Biophysical Research Communications.  2006. 346(2):379-385.
[PubMed]

Chen P, Cai Y, Yang Z, Zhou R, Zhang G, Domann F, Fang X.  Involvement of PKC, p38 MAPK and AP-2 in IL-1beta-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells.  Respirology..  2006. 11(1):18-23..

Darbro C, Lee K, Nguyan N, Domann F, Klingelhutz A.  Methylation of the p16(INK4a) promoter region in telomerase immortalized human keratinocytes co-cultured with feeder cells.  Oncogene.  2006. 25(56):7421-7433.
[PubMed]

Slane B, Aykin-Burns N, Smith B, Kalen A, Goswami P, Domann F, Spitz D.  Mutation of succinate dehydrogenase subunit C results in increased O2.-, oxidative stress, and genomic instability.  Cancer Research.  2006. 66(15):7615-7620.
[PubMed]

Yu L, Domann F.  Rapid and direct quantitative RT-PCR method to measure promoter activity.  Biotechnol Prog.  2006. 22(5):1461-1463.
[PubMed]

Lal G, Padmanabha L, Smith B, Nicholson R, Howe J, O'Dorisio M, Domann F.  RIZ1 is epigenetically inactivated by promoter hypermethylation in thyroid carcinoma.  Cancer.  2006. 107(12):2752-2759.
[PubMed]

Futscher B, Domann F.  Epigenetic Dysregulation of Maspin (SerpinB5) in Cancer Invasion and Metastasis Cancer Metastasis — Biology and Treatment.  Springer Link.  2005. 7:133-155.
[Link]

Oshiro M, Futscher B, Lisberg A, Wozniak R, Klimecki W, Domann F, Cress A.  Epigenetic regulation of the cell type-specific gene 14-3-3sigma.  Neoplasia.  2005. 7(9):799-808.
[PubMed]

Oshiro M, Kim C, Wozniak R, Jucnk D, Muñoz-Rodriguez J, Burr J, Fitzgerald M, Pawar S, Cress A, Domann F, Futscher B.  Epigenetic silencing of DSC3 is a common event in human breast cancer.  Breast Cancer Research.  2005. 7(5):R669-R680.
[PubMed]

Wang M, Zhang H, Kirk J, Weydert C, Domann F, Schafer F, Buettner G, Oberley L.  Manganese superoxide dismutase supresses hypoxic induction of hypoxia-inducible factor-1 and vascular endothelial growth factor.  Oncogene.  2005. 24(55):8154-66.
[PubMed]

Niu G, Krager K, Graham M, Hichwa R, Domann F.  Noninvasive radiological imaging of pulmonary gene transfer and expression using the human sodium iodide symporter.  Eur. J. Nucl. Med. Mol Imaging.  2005. 32(5):534-540.
[PubMed]

Devlin A, Bottiglieri T, Domann F, Lentz S.  Tissue-specific changes in H19 methylation and expression in mice with hyperhomocysteinemia..  J. Biol. Chem.  2005. 280(27):25506-25511.
[PubMed]

Futscher B, O'Meara M, Kim C, Rennels M, Lu D, Gruman L, Seftor R, Hendrix M, Domann F.  Aberrant methylation of the maspin promoter is an early event in human breast cancer.  Neoplasia.  2004. 6(4):380-9.
[PubMed]

Chaves A, Cherubini K, Herter N, Furian R, Santos D, Squier C, Domann F.  Characterization of p53 gene mutations in a Brazilian population with oral squamous cell carcinomas..  Int. J. Oncol.  2004. 24(2):295-303.
[PubMed]

Domann F, Futscher B.  Flipping the epigenetic switch.  Am. J. Pathol.  2004. 164(6):1883-1886.
[PubMed]

Gaut A, Niu G, Krager K, Graham M, Trask D, Domann F.  Genetically targeted radiotherapy of head and neck squamous cell carcinoma using the sodium-iodide symporter (NIS).  Head Neck.  2004. 26(3):265-271.
[PubMed]

Niu G, Gaut A, Ponto L, Hichwa R, Madsen M, Graham M, Domann F.  Multimodality noninvasive imaging of gene transfer using the human sodium iodide symporter.  J Nucl. Med.  2004. 45(3):445-449.
[PubMed]

Watts G, Oshiro M, Junk D, Wozniak R, Watterson S, Domann F, Futscher B.  The acetyltransferase p300/CBP-associated factor is a p53 target gene in breast tumor cells.  Neoplasia.  2004. 6(3):187-194.
[PubMed]

Nwankwo J, Spector A, Domann F.  An insertion mutation in the transcriptional regulatory region of FADS2 gives rise to human fatty acid delta-6-desaturase deficiency.  J. Lipid Res.  2003. 44(12):2311-2319.
[PubMed]

Cullen J, Hinkhouse M, Grady M, Gaut A, Liu J, Zhang Y, Weydert C, Domann F, Oberley L.  Dicumarol inhibition of NADPH:quinone oxidoreductase induces growth inhibition of pancreatic cancer via a superoxide-mediated mechanism.  Cancer Res.  2003. 63(17):5513-5520.
[PubMed]

Domann F, Futscher B.  Editorial: maspin as a molecular target for cancer therapy.  J. Urol..  2003. 169(3):1162-4.
[PubMed]

Fitzgerald M, Oshiro M, Holtan N, Krager K, Cullen J, Futscher B, Domann F.  Human pancreatic carcinoma cells activate maspin expression through loss of epigenetic control.  Neoplasia.  2003. 5(5):427-436.
[PubMed]

Duan H, Shang H, Yang J, Oberley L, Futscher B, Domann F.  MnSOD up-regulates maspin tumor suppressor gene expression in human breast and prostate cancer cells.  Antioxd. Redox Signal.  2003. 5(5):677-688.

Oshiro M, Watts G, Wozniak R, Junk D, Munoz-Rodriguez J, Domann F, Futscher B.  Mutant p53 and aberrant cytosine methylation cooperate to silence gene expression.  Oncogene.  2003. 22(23):3624-3634.
[PubMed]

He T, Weintraub N, Goswami P, Chatterjee P, Domann F, Oberley L.  Redox factor-1 mediates platelet-derived growth factor-BB induced vascular smooth muscle cell proliferation.  Am. J. Physiol. Heart Circ. Physiol..  2003. 285(2):H804-H812.
[PubMed]

Cullen J, Weydert C, Hinkhouse M, Ritchie J, Domann F, Spitz D, Oberley L.  The role of manganese superoxide dismutase in the growth of pancreatic adenocarcinoma..  Cancer Res.  2003. 63(6):1297-1303.
[PubMed]

Timmons S, Nwankwo J, Domann F.  Acetaldehyde activates Jun/AP-1 expression and DNA binding activity in human oral keratinocytes.  Oral Oncol.  2002. 38(2):281-290.
[PubMed]

Zhu C, Domann F.  Dominant negative interference of transcription factor AP-2 causes inhibition of erbB-3 expression and suppresses malignant cell growth.  Breast Cancer Res. and Treat.  2002. 71(1):47-57.
[PubMed]

Zhang Y, Zhao W, Zhang H, Domann F, Oberley L.  Overexpression of copper zinc superoxide dismutase suppresses human glioma cell growth.  Cancer Research.  2002. 62:1205-1212.
[PubMed]

Girnun G, Domann F, Moore S, Robbins M.  PPAR gamma agonists activate catalase transcription: Identification of a functional peroxisome proliferator-activated receptor response element in the rat catalase promoter.  Molecular Endocrinology.  2002. 16(12):2793-2801.
[PubMed]

Zhang H, Drake V, Xu L, Domann F, Oberley L, Kregel K.  Redox Regulation of adenovirus-induced AP-1 activation by MnSOD overexpression.  J. Virol..  2002. 76(1):355-363.
[PubMed]

Futscher B, Oshiro M, Wozniak R, Holtan N, Hanigan C, Duan H, Domann F.  Role for DNA methylation in the control of cell type specific maspin expression..  Nature Genetic.  2002. 31(2):175-179.
[PubMed]

Yang J, Buettner G, Domann F, Li Q, Engelhardt J, Weydert C, Oberley L.  v-Ha-ras mitogenic signaling through superoxide and derived reactive oxygen species.  Molecular Carcinogenesis.  2002. 33(4):206-218.
[PubMed]

Zhu C, Huang Y, Oberley L, Domann F.  A family of AP-2 proteins down-regulates manganese superoxide dismutase gene expression..  Journal of Biol. Chem.  2001. 276(17):14407-14413.
[PubMed]

Zhu C, Huang Y, Weydert C, Oberley L, Domann F.  Constitutive Activation of Transcription Factor AP-2 is Associated with Decreased MnSOD Expression in Transformed Human Lung Fibroblasts.  Antioxidants & Redox Signaling.  2001. 3(3):387-396.
[PubMed]

Zhu C, Huang Y, Broman M, Domann F.  Expression of AP-2  in SV40 immortalized human lung fibroblasts is associated with a distinct pattern of cytosine methylation in the AP-2 promoter.  Biochem. Biophys. Acta.  2001. 1519(1-2):85-91.
[PubMed]

Wang H, Qian S, Schafer F, Domann F, Oberley L, Buettner G.  Phospholipid hydroperoxide glutathione peroxidase protects against singlet oxygen-induced cell damage of photodynamic therapy.  Free Radic. Biol. Med.  2001. 30(8):825-835.
[PubMed]

Yang J, Zhao W, Duan H, Robbins M, Buettner G, Oberley L, Domann F.  v-Ha-ras oncogene up-regulates the 92 kDa typeIV collagenase gene by increasing cellular superoxide production and activating NF-B..  Free Radical Biol Med.  2001. 31(4):520-529.
[PubMed]

Yang J, Li S, Huang Y, Zhang H, Domann F, Buettner G, Oberley L.  v-Ha-Ras overexpression induces superoxide production and alters levels of primary antioxidant enzymes.  Antioxidants & Redox Signaling.  2001. 3(4):697-709.
[PubMed]

Domann F, Rice J, Hendrix M, Futscher B.  Epigenetic silencing of maspin gene expression in human breast cancers.  International Journal of Cancer.  2000. 85:805-810.
[PubMed]

Sekhar  K, Soltaninassab  S, Borelli M, Xu Z, Meredith M, Domann F, Freeman M.  Inhibition of the 26S proteasome induces expression of GLCLC, the catalytic subunit for -glutamylcysteine synthetase.  Biochem Biophys Res Comm.  2000. 270:311-217.
[PubMed]

Huang Y, He T, Domann F.  Decreased expression of manganese superoxide dismutase in transformed cells is associated with increased cytosine methylation of the SOD2 gene.  DNA & Cell Biology.  1999. 18(8):643-652.
[PubMed]

Cody D, Huang Y, Darby C, Johnson G, Domann F.  Differential DNA methylation of the p16 (INK4a)/CDKN2a promoter in human oral cancer cells and normal human oral keratinocytes.  Oral Oncology.  1999. 35:516-522.
[PubMed]

Yang J, S L, Domann F, Buettner G, Oberley L.  Superoxide generation in v-Ha-ras transduced human keratinocyte HaCaT cells.  Molecular Carcinogenesis.  1999. 26:180-188.
[PubMed]

Huang Y, Domann F.  Transcription factor AP-2 mRNA and DNA binding activity are constitutively expressed in SV40 immortalized but not normal human lung fibroblasts.  Archives Bichem. Biphys.  1999. 364(2):241-246.
[PubMed]

Huang Y, Domann F.  Redox modulation of AP-2 DNA binding activity in vitro.  Bichem. Biophys. Res. Comm.  1998. 249(307-312).
[PubMed]

Peng J, Bowden G, Domann F.  Activation of AP-1 by okadaic acid in mouse keratinocytes associated with hyperphosphorylation of c-jun.  Molecular Carcinogenesis.  1997. 18:37-43.
[PubMed]

Lam E, Zwacka R, Englehart J, Davidson B, Domann F, Oberley L.  Adenovirus-mediated manganese superoxide dismutase gene transfer to hamster cheeck pouch carcinoma cells.  Cancer Research .  1997. 24:5550-5556.
[PubMed]

Lam  E, Zwacka R, Englehart J, Davidson B, Domann F, Oberley L.  Adenovirus-mediated manganese superoxide dismutase gene transfer to hamster cheeck pouch carcinoma cells..  Cancer Research.  1997. 57(24):5550-5556.
[PubMed]

Kelley E, Domann F, Buettner G, Burns C, Oberley L.  Increased efficacy of photofrin photosensitization of oral squamous cell carcioma by the pro-oxidants iron and ascorbate.  Eur. J. Photochem. Photobiol. B:Biology.  1997. 40:273-277.
[PubMed]

Kelley E, Domann F, Buettner G, Burns C, Oberley L.  Increased efficacy of photofrin photosensitization of oral squamous cell carcioma by the pro-oxidants iron and ascorbate.  Eur. J. Photochem. Photobiol. B:Biology.  1997. 40:273-277.
[PubMed]

Kelley E, Domann F, Buettner G, Burns C, Oberley L.  Increased efficacy of photofrin photosensitization of oral squamous cell carcioma by the pro-oxidants iron and ascorbate.  Eur. J. Photochem. Photobiol. B:Biology.  1997. 40:273-277.
[PubMed]

Huang Y, Peng J, Oberley L, Domann F.  Transcriptional inhibition of manganese superoxide dismutase (SOD2) gene expression by DNA methylation of the 5' CpG island.  Free Radic Biol Med.  1997. 23(2):314-320.
[PubMed]

Peng J, Bowden G, Domann F.  Transcriptional regulation of the c-jun proto-oncogene in mouse keratinocytes by the skin tumor promoter okadaic acid.  Molecular Carcinogenesis.  1997. 18:37-43.

Peng J, Bowden G, Domann F.  Transcriptional regulation of the c-jun proto-oncogene in mouse keratinocytes by the skin tumor promoter okadaic acid.  Molecular Carcinogenesis.  1997. 18:37-43.

Zhong W, Oberley L, Oberley T, Domann F, St.Clair D.  Inhibition of cell growth and sensitization to oxidative damage by overexpression of manganese superoxide dismutase in rat glioma cells.  Cell Growth and Differentiation.  1996. 7:1175-1186.
[PubMed]

Powell W, Domann F, Mitchen J, Matrisian L, Nagle R, Bowden G.  Matrilysin expression in the involuting rat ventral prostate.  Prostate.  1996. 29(3):159-168.
[PubMed]

Domann F, Martinez M.  An alternative to cloning cylinders for isolation of adherent cell colonies.  BioTechniques.  1995. 18:594.

Liu J, Domann F, Oberley L.  The use of RT-PCR to distinguish between plasmid MnSOD transcripts and endogenous MnSOD mRNA. Biochem..  Biophys. Res. Commun.  1995. 216(2):610-618.

Futscher B, Abbaszadegan M, Domann F, Dalton W.  Analysis of MRP mRNA in mitaxantrone-selected, multidrug resistant human tumor cells.  Biochem. Pharmacol.  1994. 47(9):1601-1606.

Domann F, Levy J, Finch J, Bowden G.  Constitutive AP-1 DNA binding and transactivating ability of malignant but not benign mouse epidermal cells.  Molecular Carcinogenesis.  1994. 9(2):61-66.
[PubMed]

Bowden G, Schneider B, Domann F, Kulesz-Martin M.  Oncogene activation and tumor suppressor gene inactivation during multistage mouse skin carcinogenesis.  Cancer Research.  1994. 54(7):1882s-1885s.

Domann F, Freitas M, Gould M, Clifton K.  Quantifying the frequency of radiogenic cancer initiation per clonogenic rat thyroid epithelial cell in vivo.  Radiation Res.  1994. 137(3):330-337.
[PubMed]

Domann F, Levy J, Brown P, Birrer M, Bowden G.  Stable expression of a dominant negative c-jun gene in malignant mouse epidermal cells blocks cellular AP-1 activity and tumor formation.  Cell Growth and Differentiation.  1994. 5(1):9-16.
[PubMed]

Nelson M, Domann F, Bowden G, Hooser S, Fernando Q, Carter D.  Effects of acute and sub-chronic exposure of topically applied fullerene extracts on the mouse skin.  Toxicol. Ind. Health.  1993. 9(4):623-630.
[PubMed]

Clifton K, Domann F, Groch K.  On the cells of origin of radiogenic thyroid cancer: New studies based on an old idea..  J. Radiat. Res.  1991. 32:143-155.
[PubMed]

Clifton K, Groch K, Domann F.  Thyroid clonogen biology and carcinogenesis.  Prog. Clin. Biol. Res.  1991. 369:173-183.
[PubMed]

Domann F, Mitchen J, Clifton K.  Restoration of thyroid function following total thyroidectomy and quantitative thyroid cell transplantation.  Endocrinology.  1990. 127(6):2673-2678.
[PubMed]

Seed T, Kaspar L, Domann F, Niiro G, LeBuis D.  Developmental and radiobiologic characteristics of canine multinucleated, osteoclast-like cells generated in vitro from canine bone marrow.  Scanning Microsc.  1988. 2(3):1599-1611.

Watanabe H, Tanner M, Domann F, Gould M, Clifton K.  Inhibition of carcinoma formation and of vascular invasion in grafts of radiation-initiated thyroid clonogens by unirradiated thyroid cells.  Carcinogenesis.  1988. 9(8):1329-1335.
[PubMed]

Date Last Modified: 06/07/2014 - 21:56:23