Pharmacology

Minnetta V. Gardinier, PhD

Portrait

Associate Dean for Recruitment & Professional Development, Graduate College
Associate Professor of Pharmacology

Contact Information

Primary Office: 2-550 Bowen Science Building
Iowa City, IA 52242
Primary Office Phone: 319-335-6735

Office: 205 GILH
Iowa City, IA 52242
Office Phone: 319-335-2147

Email: m-gardinier@uiowa.edu

Education

BS, Biology, LeMoyne College, Syracuse, NY
PhD, Biochemistry & Molecular Biology, Louisiana State University Medical Center, New Orleans, LA

Postdoctoral Research Associate, Pediatrics, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland

Research Summary

Oligodendroglial Cell Biology and CNS Myelination: Myelin in the central nervous system (CNS) is essential for normal nervous system development and function. Multiple sclerosis (MS) is the #1 neurological disease that afflicts young adults and results in targeted destruction of myelin, oligodendrocytes, and eventually, neurons in the CNS. Oligodendrocytes in the CNS serve to synthesize and maintain myelin sheaths, which are highly complex, multilamellar membranous structures essential to normal nervous system function. Myelination is a tightly regulated event that must occur during perinatal and early postnatal CNS development in vertebrates. Disruption of myelination during development can lead to mental retardation, movement disorders, and other neurological deficits. Our research efforts focus on gaining a better understanding of the molecular and cellular biology of oligodendrocytes and the process of myelination. Our studies consider issues of cell polarity and membrane targeting for the distribution of proteins on the oligodendroglial cell surface and myelin membrane surrounding neurons. In particular, we are studying myelin/oligodendrocyte glycoprotein (MOG), which is specifically targeted for immune attack in MS. Our structure-function analyses of MOG have revealed sequence motifs in the cytoplasmic domain of this protein that are necessary for its membrane targeting and its ability to undergo endocytosis for reuptake back into the cell. Recently, we identified stathmin, a phosphoregulatory protein affecting microtubule dynamics, as a cytosolic binding partner for MOG, and we are now investigating the significance of these interactions. Surprisingly, the human MOG gene expresses alternatively spliced mRNAs that are translated to unique isoforms lacking these targeting and endocytic motifs. These novel MOG isoforms found in human CNS may exhibit altered developmental expression and may interact with alternative cytosolic partners. Studies in these areas are ongoing. We utilize an array of techniques encompassing cell biology, molecular biology, and protein chemistry. We hope that our efforts will aid in designing improved therapies for MS and myelin- related developmental neurological disorders. MOG and stathmin colocalize at the inner face of the plasma membrane.

All Publications

Waseen A, Menon K, Gardinier M.  Strange bedfellows – myelin/oligodendrocyte glycoprotein and stathmin, a microtubule-destabilizing protein.  2008. 104(Suppl):PSM08-24.

Allamargot C, Gardinier M.  Alternative isoforms of myelin/oligodendrocyte glycoprotein with variable cytoplasmic domains are expressed in human brain.  J Neurochem.  2007 April. 101(2):298-312.
[Link]

Waseen A, Menon K, Gardinier M.  The C-terminus of myelin/oligodendrocyte glycoprotein interacts with stathmin, a microtubule destabilizing protein.  Amer. Soc. Cell Biol..  2006. Abst. 3792.

Gardinier M, Allamargot C, Koch M, Lee Y, Menon K.  Myelin/oligodendrocyte glycoprotein: receptor endocytosis, membrane targeting, and an intracellular partner.  J. Neurochem.  2005. 84 (Suppl):Abst. PSM8-03.

Allamargot C, Lee Y, Gardinier M.  Human myelin/oligodendrocyte glycoprotein (MOG): alternative splice variants at the membrane.  Soc Neurosci .  2004. Abst. 30 Prog. No. 722.7.

Allamargot C, Lee Y, Gardinier M.  Human myelin/oligodendrocyte glycoprotein (MOG): expression of alternative isoforms.  Gordon Research Conference on “Myelin”, Il Ciocco, Italy.  2004. 

Koch M, Gardinier M.  Membrane targeting and internalization of MOG is perturbed by alanine mutagenesis of its trafficking motifs.  Amer. Soc. Cell Biol..  2004. Abst. 2416.

Menon K, Gardinier M.  The cytoplasmic domain of MOG interacts with stathmin, a regulator of microtubule dynamics.  Amer. Soc. Cell Biol.  2004. Abst. 946.

Koch M, Gardinier M.  C-terminal motifs mediate membrane targeting and endocytosis of myelin/oligodendrocyte glycoprotein.  Amer. Soc. Cell Biol.  2003. Abst. 2019.

Allamargot C, Lee Y, Gardinier M.  Human myelin/oligodendrocyte glycoprotein (MOG): alternative isoforms and their membrane localization.  Soc Neurosci .  2003. Abst. 29 Prog. No. 905.9.

Koch M, Gardinier M.  Endocytosis of myelin/oligodendrocyte glycoprotein and internalization signals in the C-terminal tail.  Amer. Soc. Cell Biol..  2002. Abst. 2048.

Allamargot C, Gardinier M.  Human myelin/oligodendrocyte glycoprotein: alternative splicing affects basolateral targeting and endocytosis signals.  Amer. Soc. Cell Biol..  2002. Abst. 492.

Kroepfl J, Gardinier M.  Mutually exclusive apicobasolateral sorting of two oligodendroglial membrane proteins, proteolipid protein and myelin/oligodendrocyte glycoprotein, in Madin-Darby canine kidney cells.  J Neurosci Res.  2001 December 15. 66(6):1140-1148.
[Link]

Kroepfl J, Gardinier M.  Identification of a basolateral membrane targeting signal within the cytoplasmic domain of myelin/oligodendrocyte glycoprotein.  J Neurochem.  2001 June. 77(5):1301-1309.
[Link]

Allamargot C, Gardinier M.  Expression of myelin/oligodendrocyte glycoprotein isoforms in humans.  Soc Neurosci .  2001. Abst. 27 Program No. 157.1.

Koch M, Gardinier M.  Myelin/oligodendrocyte glycoprotein (MOG) undergoes endocytosis, and mutations identify potential endocytosis signals.  Amer. Soc. Cell Biol.  2001. Abst. B429.

Kroepfl J, Gardinier M.  Apico-basolateral sorting of two myelin membrane proteins: shared mechanisms between oligodendrocytes and MDCK cells?.  Amer. Soc. Cell Biol.  2000. Abstr. L99.

Kroepfl J, Gardinier M.  Myelin membrane proteins are sorted to specific domains using common targeting mechanisms.  J. Neurochem..  2000. 74 (Suppl):S32C.

Gardinier M, Kroepfl J.  Myelin/oligodendrocyte glycoprotein, myelin proteins, and cell polarity.  Gordon Research Conference on “Myelin”, Il Ciocco, Italy.  2000. 

Brehm U, Piddlesden S, Gardinier M, Linington C.  Epitope specificity of demyelinating monoclonal autoantibodies directed against the human myelin oligodendrocyte glycoprotein (MOG).  J Neuroimmunol.  1999 June 1. 97(1-2):9-15.
[Link]

Kroepfl J, Gardinier M.  Myelin/oligodendrocyte glycoprotein exhibits a polarized distribution on cell surfaces.  J. Neurochem.  1999. 72 (Suppl):S18B.

Kroepfl J, Gardinier M.  Polarized sorting of myelin/oligodendrocyte glycoprotein.  Mol. Biol. Cell.  1998. 9 (Suppl):89a (Abst 513).

Nikcevich K, Gordon K, Tan L, Kroepfl J, Hurst S, Gardinier M, Barrett T, Miller S.  Interferon-g-activated primary murine astrocytes express B7 costimulatory molecules and prime naive antigen-specific T cells.  J Immunol.  1997 January 15. 158(2):614-621.
[Link]

Gardinier M, Ballenthin P.  A subpopulation of neutrophils express myelin/oligodendrocyte glycoprotein on the cell surface.  ISN Satellite and Kroc Symposium on “Frontiers of Myelin Biology and Demyelinating Disease,” Mystic, CT.  1997. 

Gardinier M, Ballenthin P, Kroepfl J, Viise L.  Myelin/oligodendrocyte glycoprotein - a molecular analysis. In: Cell Biology and Pathology of Myelin: Evolving Biological Concepts and Therapeutic Approaches.  Plenum Press, New York.  1997. 

Kroepfl J, Gardinier M.  Myelin/oligodendrocyte glycoprotein - evidence for cytoplasmic interactions.  ISN Satellite and Kroc Symposium on “Frontiers of Myelin Biology and Demyelinating Disease,” Mystic, CT.  1997. 

Gardinier M, Ballenthin P.  Myelin/oligodendrocyte glycoprotein is expressed on human neutrophils.  Soc. Neurosci..  1997. 23(Part 1):585.

Kroepfl J, Viise L, Charron A, Linington C, Gardinier M.  Investigation of myelin/oligodendrocyte glycoprotein membrane topology.  J Neurochem.  1996 November. 67(5):2219-2222.
[Link]

Ballenthin P, Gardinier M.  Myelin/oligodendrocyte glycoprotein is alternatively spliced in humans but not mice.  J Neurosci Res.  1996 October 15. 46(2):271-281.
[Link]

Solly S, Thomas J, Monge M, Demerens C, Lubetzki C, Gardinier M, Matthieu J, Zalc B.  Myelin/oligodendrocyte glycoprotein (MOG) expression is associated with myelin deposition. .  Glia.  1996 September. 18(1):39-48.
[Link]

Ballenthin P, Gardinier M.  Identification of alternatively spliced MOG transcripts.  J. Neurochem.  1996. 66(Suppl 1):S87B.

kroepfl J, Viise L, Linington C, Gardinier M.  Membrane topology of myelin/oligodendrocyte glycoprotein.  Mol. Biol Cell.  1996. 7(Suppl):597a (Abst3472).

 .  Membrane topology of myelin/oligodendrocyte glycoprotein.  Mol. Biol Cell.  1996. 7(Suppl):597a (Abst 3472).

Adelmann M, Wood J, Benzel I, Fiori P, lassmann H, Matthieu J, Gardinier M, Dornmair K, Linington C.  The N-terminal domain of the myelin oligodendrocyte glycoprotein (MOG) induces acute demyelinating experimental autoimmune encephalomyelitis in the Lewis rat. .  J neuroimmunol.  1995 December. 63(1):17-27.
[Link]

Gardinier M, Viise L, Kroepfl J, Charron A.  MOG expression in stably transfected HEK293 cells.  J. Neurochem.  1995. 64(Suppl):S18D.

Amor S, Groome N, Linington C, Morris M, Dornmair k, Gardinier M, Matthieu J, Baker D.  Identification of epitopes of myelin oligodendrocyte glycoprotein for the induction of experimental allergic encephalomyelitis in SJL and Biozzi AB/H mice..  J Immunol.  1994 November 15. 153(10):4349-4356.
[Link]

Gardinier M.  Characterization of human and mouse myelin/oligodendrocyte glycoprotein cDNAs. Gordon Research Conference on “Myelin”.  Oxnard, CA.  1994. 

Gardinier M, Viise L, Kroepfl J, Charron A.  Expression of myelin/oligodendrocyte glycoprotein in stably transfected HEK293 cells.  Collomed Symposium, Lausanne, Switzerland.  1994. 

Gardinier M, Matthieu J.  Cloning and cDNA sequence analysis of myelin/oligodendrocyte glycoprotein: a novel member of the immunoglobulin gene superfamily..  Schweiz Arch Neurol Psychiatr .  1993. 144(3):201-207.
[PubMed]

Linington C, Popovici R, Gardinier M.  Human T cell response to myelin/oligodendrocyte glycoprotein (MOG): molecular mimicry with an epitope of measles L protein.  Soc. Neurosci..  1993. (Abstr 19(Part 1):842.

Gardinier M, Matthieu J.  Murine and human MOG are highly conserved: cDNA analysis.  Trans. Am. Soc. Neurochem..  1993. 24:234.

Amiguet P, Gardinier M, Zanetta J, Matthieu J.  Purification and partial structural and functional characterization of mouse myelin/oligodendrocyte glycoprotein.  J Neurochem.  1992 May. 58(5):1676-1682.
[PubMed]

Gardinier M, Matthieu J.  MOG - A new immunoglobulin gene superfamily member?.  Trans. Am. Soc. Neurochem..  1992. 23:137.

Gardinier M, Cherbuin S, Matthieu J.  Myelin/oligodendrocyte glycoprotein: cDNA sequence and mRNA expression..  USGEB Meeting, Experientia.  1992. 48:A15.

Malotka J, Waehneldt T, Matthieu J, Gardinier M.  Identification and characterization of cDNAs for trout 36 kDa CNS myelin protein..  Soc. Neurosci. .  1991. Abstr 17(Part 1):375.

Gardinier M, Amiguet P, Linington C, Matthieu J.  Identification of a rat cDNA clone for myelin/oligodendrocyte glycoprotein..  Soc. Neurosci..  1991. Abstr. 17(Part 1):758.

Amiguet P, Gardinier M, Matthieu J.  Myelin/oligodendrocyte glycoprotein (MOG): localization, developmental expression, and isolation.  USGEB Meeting, Experientia.  1991. 47:A2.

Macklin W, Gardinier M, Obeso Z, King K, Wight P.  Mutations in the myelin proteolipid protein gene alter oligodendrocyte gene expression in jimpy and jimpymsd mice.  J Neurochem.  1991 January. 56(1):163-171.
[PubMed]

Matthieu J, Tosic M, Gardinier M.  Abnormal regulation of myelin protein genes during development of the myelin deficient mouse. In: Myelination and Dysmyelination.  Ann, New York, Acad. Sci..  1990. 605:240-247.

Gardinier M, Tosic M, Matthieu J.  Developmental expression of major myelin protein genes in mld mice. .  Trans. Am. Soc. Neurochem.  1990. 21:202.

Gardinier M, Macklin W.  Developmental expression of the myelin proteolipid protein gene. In: Cellular and Molecular Biology of Myelination.  Springer-Verlag, Berlin.  1990. H43:517-532.

Matthieu J, Tosic M, Gardinier M.  Myelin-deficient mice: from phenotype to genotype. .  USGEB Meeting, Experientia.  1990. 46(A7).

Gardinier M, Tosic M, Matthieu J.  Pleiotropic effects on developmental expression of major myelin protein genes in mld mice..  J. Cell Biochem.  1990. 14F(Suppl):CQ104.

Tosic M, Gardinier M, Matthieu J.  Regulation of the duplicated myelin basic protein gene expression in myelin deficient mice. .  USGEB Meeting, Experientia.  1990. 46:A71.

Macklin W, Gardinier M, Obeso Z.  Structure and expression of the mouse myelin proteolipid protein gene. In: Myelination and Dysmyelination.  Ann, New York, Acad. Sci.  1990. 605:183-193.

Macklin W, Gardinier M, King K, Obeso Z.  Myelin proteolipid protein gene structure and expression in jimpymsd mice. .  J. Cell Biol. .  1989. 107(6, pt3):514a (Abst 2900).

Gardinier M, Macklin W.  Myelin proteolipid protein gene expression in jimpy and jimpymsd mice.  J Neurochem.  1988. 51(2):360-369.
[PubMed]

Gardinier M, King K, Macklin W.  Proteolipid protein gene and its transcription in jimpymsd mice.  Trans. Am. Soc. Neurochem. .  1988. 19:319.

Gardinier M, Macklin W.  Proteolipid protein gene expression in jimpy and jimpymsd mice..  J. Cell Biochem..  1988. 12D(Suppl):N511.

Backlin W, Gardinier M, King K, Kampf K.  An AG --> GG transition at a splice site in the myelin proteolipid protein gene in jimpy mice results in the removal of an exon.  FEBS Lett.  1987 November 2. 223(417-421).
[Link]

Gardinier M, Macklin W.  A comparison of PLP gene expression in jp and jpmsd mice. .  J. Neurochem.  1987. 48(Suppl):130B.

Macklin W, Gardinier M, Deininger P, Sorg B, Campagnoni C.  Myelin proteolipid protein expression in normal and jimpy mice. In: A Multidisciplinary Approach to Myelin Diseases.  Plenum Press, New York.  1987. 

Macklin W, Gardinier M, Deininger P, Campagnoni C.  Sequence of the exons of the mouse myelin proteolipid protein gene..  J. Neurochem..  1987. 48(Suppl):32C.

Macklin W, Campagnoni C, Deininger P, Gardinier M.  Structure and expression of the mouse myelin proteolipid protein gene.  J Neurosci Res.  1987. 18(3):383-394.
[PubMed]

Gardinier M, Macklin W, Diniak A, Deininger P.  Characterization of myelin proteolipid mRNAs in normal and jimpy mice.  Mol Cell Biol.  1986 November. 6(3755-3762).
[Link]

Deininger P, Gardinier M, Macklin W.  Myelin proteolipid expression in normal and jimpy mouse brain development.  J. Cell Biochem..  1986. 10D(Suppl):0145.

Gardinier M, Deininger P, Macklin W.  Myelin proteolipid mRNAs in normal and jimpy brain development.  Trans. Am. Soc. Neurochem..  1986. 17:109.

Sheiness D, Gardinier M.  Expression of a proto-oncogene (Proto-myb) in hemopoietic tissues of mice.  Mol Cell Biol.  1984. 4:1206-1212.

Sheiness D, Gardinier M.  Expression of a proto-oncogene (proto-myb) in hemopoietic tissues of the mouse.  J Cell Biochem.  1984. 8A(Suppl):167.

Date Last Modified: 06/07/2014 - 21:56:23