Stead Family Department of Pediatrics

Peter Rubenstein, PhD

Portrait

Professor of Biochemistry
Professor of Internal Medicine, Pediatrics

Contact Information

Primary Office: 4-730 BSB
Iowa City, IA 52242
Primary Office Phone: 319-335-7911

Email: peter-rubenstein@uiowa.edu
Web: Rubenstein Laboratory

Education

AB, Biochemistry, University of California, Berkeley
MA, Biochemistry and Molecular Biology, Harvard University
PhD, Biochemistry and Molecular Biology, Harvard University

Post Doctoral, Biochemistry & Biophysics, Univ. of California School of Medicine-SF
Post Doctoral, Biochemistry & Biophysics, Univ of California School of Medicine-SF

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Biochemistry PhD
Interdisciplinary Graduate Program in Molecular and Cellular Biology
Interdisciplinary Graduate Program in Translational Biomedicine
Medical Scientist Training Program

Research Summary

Our laboratory investigates the biochemistry of actin and the actin binding proteins that modulate its function within the cell. In particular, we are interested in the conformational changes in actin necessary for its polymerization, the forces that stabilize actin monomers within the actin filament, and the manner in which actin filament modulating proteins such as cofilin, Arp2/3 complex and profilin carry out their roles. Our approach is to use yeast actin and the yeast actin cytoskeleton as a model system. Based on hypotheses concerning actin function and actin dynamics, we make mutant actin constructs using site-directed mutagenesis, express the actin in yeast as the only actin in the cell, and determine phenotypes associated with the actin mutation such as altered growth rate, altered endocytosis, and altered actin deposition. We then purify the actin and determine its in vitro behavior using a series of biochemical and biophysical approaches such as electron microscopy and fluorescence spectroscopy. We then try to correlate the effects of the mutations we observe in vitro with the phenotypes associated with these mutations in vivo. With this basic system, we are currently focusing on two projects. We have shown that although the proteins are 90% identical, muscle and yeast actins display distinct differences in their kinetics and ability to interact with different actin binding proteins that can have a substantial effect on actin filament dynamics. To investigate the basis for these differences, we have made a set of yeast/muscle hybrid actins with one yeast half and one muscle half to try to determine which part of the actin is responsible for the behavioral differences seen between the two parent actins. Second, it has been found that six different actin mutations cause autosomal dominant hearing loss in humans. We have cloned each of these into yeast actin and are assessing the effects of these mutations in vivo and in vitro with the goal of gaining insight into altered actin interactions that might result in deafness.

All Publications

Rubenstein P, Wen K.  Insights into the effects of disease-causing mutations in human actins.  Cytoskeleton (Hoboken).  2014 February 25. 
[PubMed]

Wen K, McKane M, Rubenstein P.  Importance of a Lys113-Glu195 Intermonomer Ionic Bond in F-actin Stabilization and Regulation by Yeast Formins Bni1p and Bnr1p.  The Journal of Biological Chemistry.  2013 June. 288(26):19140-53.
[PubMed]

Johnston J, Wen K, Keppler-Noreuil K, McKane M, Maiers J, Greiner A, Sapp J.  Functional Analysis of a De Novo ACTB Mutation in a Patient with Atypical Baraitser-Winter Syndrome.  Human Mutation.  2013 May. doi: 10.1002/humu.22350.
[PubMed]

Lee C, Lou J, Wen K, McKane M, Eskin S, Ono S, Chien S, Rubenstein P, Zhu C, McIntire L.  Actin depolymerization under force is governed by lysine 113:glutamic acid 195-mediated catch-slip bonds.  Proceedings of the National Academy of Sciences of the United States of America.  2013 March. 110(13):5022-7.
[PubMed]

Rubenstein P, Mayer E.  Familial visceral myopathies: From symptom-based syndromes to actin-related diseases.  Gastroenterology.  2012 December. 143(6).
[PubMed]

Glenn N, McKane M, Kohli V, Wen K, Rubenstein P, Bartman T, Sumanas S.  The W-loop of alpha-cardiac actin is critical for heart function and endocardial cushion morphogenesis in zebrafish.  Molecular and Cellular Biology.  2012 September. 32(17):3527-40.
[PubMed]

Malloy L, Wen K, Pierick A, Wedemeyer E, Bergeron S, Vanderpool N, McKane M, Rubenstein P, Bartlett H.  Thoracic aortic aneurysm (TAAD)-causing mutation in actin affects formin regulation of polymerization.  The Journal of Biological Chemistry.  2012 August. 287(34):28398-408.
[PubMed]

Kruth K, Rubenstein P.  Two deafness-causing (DFNA20/26) actin mutations affect Arp2/3-dependent actin regulation.  The Journal of Biological Chemistry.  2012 August. 287(32):27217-26.
[PubMed]

Wen K, McKane M, Stokasimov E, Rubenstein P.  Mutant profilin suppresses mutant actin-dependent mitochondrial phenotype in Saccharomyces cerevisiae.  The Journal of Biological Chemistry.  2011 December. 286(48):41745-57.
[PubMed]

Stark B, Wen K, Allingham J, Rubenstein P, Lord M.  Functional adaptation between yeast actin and its cognate myosin motors.  The Journal of Biological Chemistry.  2011 September. 286(35):30384-92.
[PubMed]

Bergeron S, Wedemeyer E, Lee R, Wen K, McKane M, Pierick A, Berger A, Rubenstein P, Bartlett H.  Allele-specific effects of thoracic aortic aneurysm and dissection alpha-smooth muscle actin mutations on actin function.  The Journal of Biological Chemistry.  2011 April. 286(13):11356-69.
[PubMed]

Kudryashov D, Grintsevich E, Rubenstein P, Reisler E.  A nucleotide state-sensing region on actin.  The Journal of Biological Chemistry.  2010 August. 285(33):25591-601.
[PubMed]

Wen K, McKane M, Stokasimov E, Fields J, Rubenstein P.  A potential yeast actin allosteric conduit dependent on hydrophobic core residues val-76 and trp-79.  The Journal of Biological Chemistry.  2010 July. 285(27):21185-94.
[PubMed]

Bergeron S, Zhu M, Thiem S, Friderici K, Rubenstein P.  Ion-dependent polymerization differences between mammalian beta- and gamma-nonmuscle actin isoforms.  The Journal of Biological Chemistry.  2010 May. 285(21):16087-95.
[PubMed]

Wen K, Rubenstein P, DeMali K.  Vinculin nucleates actin polymerization and modifies actin filament structure.  The Journal of Biological Chemistry.  2009 October. 284(44):30463-73.
[PubMed]

Stokasimov E, Rubenstein P.  Actin isoform-specific conformational differences observed with hydrogen/deuterium exchange and mass spectrometry.  The Journal of Biological Chemistry.  2009 September. 284(37):25421-30.
[PubMed]

Morín M, Bryan K, Mayo-Merino F, Goodyear R, Mencía A, Modamio-Høybjør S, del Castillo I, Cabalka J, Richardson G, Moreno F, Rubenstein P.  In vivo and in vitro effects of two novel gamma-actin (ACTG1) mutations that cause DFNA20/26 hearing impairment.  Human Molecular Genetics.  2009 August. 18(16):3075-89.
[PubMed]

Bryan K, Rubenstein P.  Allele-specific effects of human deafness gamma-actin mutations (DFNA20/26) on the actin/cofilin interaction.  The Journal of Biological Chemistry.  2009 July. 284(27):18260-9.
[PubMed]

Wen K, Rubenstein P.  Differential regulation of actin polymerization and structure by yeast formin isoforms.  The Journal of Biological Chemistry.  2009 June. 284(25):16776-83.
[PubMed]

Scoville D, Stamm J, Altenbach C, Shvetsov A, Kokabi K, Rubenstein P, Hubbell W, Reisler E.  Effects of binding factors on structural elements in F-actin.  Biochemistry.  2009 January. 48(2):370-8.
[PubMed]

Stokasimov E, McKane M, Rubenstein P.  Role of intermonomer ionic bridges in the stabilization of the actin filament.  The Journal of Biological Chemistry.  2008 December. 283(50):34844-54.
[PubMed]

Wen K, McKane M, Houtman J, Rubenstein P.  Control of the ability of profilin to bind and facilitate nucleotide exchange from G-actin.  The Journal of Biological Chemistry.  2008 April. 283(14):9444-53.
[PubMed]

Shvetsov A, Galkin V, Orlova A, Phillips M, Bergeron S, Rubenstein P, Egelman E, Reisler E.  Actin hydrophobic loop 262-274 and filament nucleation and elongation.  Journal of Molecular Biology.  2008 January. 375(3):793-801.
[PubMed]

Scoville D, Stamm J, Toledo-Warshaviak D, Altenbach C, Phillips M, Shvetsov A, Rubenstein P, Hubbell W, Reisler E.  Hydrophobic loop dynamics and actin filament stability.  Biochemistry.  2006 November. 45(45):13576-84.
[PubMed]

McKane M, Wen K, Meyer A, Rubenstein P.  Effect of the substitution of muscle actin-specific subdomain 1 and 2 residues in yeast actin on actin function.  The Journal of Biological Chemistry.  2006 October. 281(40):29916-28.
[PubMed]

Bryan K, Wen K, Zhu M, Rendtorff N, Feldkamp M, Tranebjaerg L, Friderici K, Rubenstein P.  Effects of human deafness gamma-actin mutations (DFNA20/26) on actin function.  The Journal of Biological Chemistry.  2006 July. 281(29):20129-39.
[PubMed]

Shvetsov A, Stamm J, Phillips M, Warshaviak D, Altenbach C, Rubenstein P, Hideg K, Hubbell W, Reisler E.  Conformational dynamics of loop 262-274 in G- and F-actin.  Biochemistry.  2006 May. 45(20):6541-9.
[PubMed]

Chen W, Wen K, Sens A, Rubenstein P.  Differential interaction of cardiac, skeletal muscle, and yeast tropomyosins with fluorescent (pyrene235) yeast actin.  Biophysical Journal.  2006 February. 90(4):1308-18.
[PubMed]

McKane M, Wen K, Boldogh I, Ramcharan S, Pon L, Rubenstein P.  A mammalian actin substitution in yeast actin (H372R) causes a suppressible mitochondria/vacuole phenotype.  The Journal of Biological Chemistry.  2005 October. 280(43):36494-501.
[PubMed]

Rubenstein P, Wen K.  Lights, camera, actin.  IUBMB life.  2005 October. 57(10):683-7.
[PubMed]

Wen K, Rubenstein P.  Acceleration of yeast actin polymerization by yeast Arp2/3 complex does not require an Arp2/3-activating protein.  The Journal of Biological Chemistry.  2005 June. 280(25):24168-74.
[PubMed]

Lu X, Bryant M, Bryan K, Rubenstein P, Kawai M.  Role of the N-terminal negative charges of actin in force generation and cross-bridge kinetics in reconstituted bovine cardiac muscle fibres.  The Journal of Physiology.  2005 April. 564(Pt 1):65-82.
[PubMed]

Bryan K, Rubenstein P.  An intermediate form of ADP-F-actin.  The Journal of Biological Chemistry.  2005 January. 280(2):1696-703.
[PubMed]

Orlova A, Shvetsov A, Galkin V, Kudryashov D, Rubenstein P, Egelman E, Reisler E.  Actin-destabilizing factors disrupt filaments by means of a time reversal of polymerization.  Proceedings of the National Academy of Sciences of the United States of America.  2004 December. 101(51):17664-8.
[PubMed]

Bartman T, Walsh E, Wen K, McKane M, Ren J, Alexander J, Rubenstein P, Stainier D.  Early myocardial function affects endocardial cushion development in zebrafish.  PLoS Biology.  2004 May. 2(5):E129.
[PubMed]

Rubenstein P.  Alcohol metabolism (sung to the tune of amazing grace).  Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology.  2004 March. 32(2):30.
[PubMed]

Wen K, Blake M, Rubenstein P.  Neisseria gonorrhoeae porin, P.IB, causes release of ATP from yeast actin.  Journal of Muscle Research and Cell Motility.  2004. 25:343-50.
[PubMed]

Wen K, Rubenstein P.  Biochemical consequences of the cardiofunk (R177H) mutation in yeast actin.  The Journal of Biological Chemistry.  2003 November. 278(48):48386-94.
[PubMed]

Vorobiev S, Strokopytov B, Drubin D, Frieden C, Ono S, Condeelis J, Rubenstein P, Almo S.  The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism.  Proceedings of the National Academy of Sciences of the United States of America.  2003 May. 100(10):5760-5.
[PubMed]

Kusner D, Barton J, Wen K, Wang X, Rubenstein P, Iyer S.  Regulation of phospholipase D activity by actin. Actin exerts bidirectional modulation of Mammalian phospholipase D activity in a polymerization-dependent, isoform-specific manner.  The Journal of Biological Chemistry.  2002 December. 277(52):50683-92.
[PubMed]

Wong W, Gerson J, Rubenstein P, Reisler E.  Thin filament regulation and ionic interactions between the N-terminal region in actin and troponin.  Biophysical Journal.  2002 November. 83(5):2726-32.
[PubMed]

Wen K, Yao X, Rubenstein P.  GTP-yeast actin.  The Journal of Biological Chemistry.  2002 October. 277(43):41101-9.
[PubMed]

Shvetsov A, Musib R, Phillips M, Rubenstein P, Reisler E.  Locking the hydrophobic loop 262-274 to G-actin surface by a disulfide bridge prevents filament formation.  Biochemistry.  2002 September. 41(35):10787-93.
[PubMed]

Musib R, Wang G, Geng L, Rubenstein P.  Effect of polymerization on the subdomain 3/4 loop of yeast actin.  The Journal of Biological Chemistry.  2002 June. 277(25):22699-709.
[PubMed]

Yao X, Nguyen V, Wriggers W, Rubenstein P.  Regulation of yeast actin behavior by interaction of charged residues across the interdomain cleft.  The Journal of Biological Chemistry.  2002 June. 277(25):22875-82.
[PubMed]

Yao X, Rubenstein P.  F-actin-like ATPase activity in a polymerization-defective mutant yeast actin (V266G/L267G).  The Journal of Biological Chemistry.  2001 July. 276(27):25598-604.
[PubMed]

Wen K, Kuang B, Rubenstein P.  Tropomyosin-dependent filament formation by a polymerization-defective mutant yeast actin (V266G,L267G).  The Journal of Biological Chemistry.  2000 December. 275(51):40594-600.
[PubMed]

Wen K, Giardina P, Blake M, Edwards J, Apicella M, Rubenstein P.  Interaction of the gonococcal porin P.IB with G- and F-actin.  Biochemistry.  2000 July. 39(29):8638-47.
[PubMed]

Kim E, Wriggers W, Phillips M, Kokabi K, Rubenstein P, Reisler E.  Cross-linking constraints on F-actin structure.  Journal of Molecular Biology.  2000 June. 299(2):421-9.
[PubMed]

Hansen J, Marner J, Pavlov D, Rubenstein P, Reisler E.  Structural transition at actin's N-terminus in the actomyosin cross-bridge cycle.  Biochemistry.  2000 February. 39(7):1792-9.
[PubMed]

Yao X, Grade S, Wriggers W, Rubenstein P.  His(73), often methylated, is an important structural determinant for actin. A mutagenic analysis of HIS(73) of yeast actin.  The Journal of Biological Chemistry.  1999 December. 274(52):37443-9.
[PubMed]

Cheng D, Marner J, Rubenstein P.  Interaction in vivo and in vitro between the yeast fimbrin, SAC6P, and a polymerization-defective yeast actin (V266G and L267G).  The Journal of Biological Chemistry.  1999 December. 274(50):35873-80.
[PubMed]

Kalhor H, Niewmierzycka A, Faull K, Yao X, Grade S, Clarke S, Rubenstein P.  A highly conserved 3-methylhistidine modification is absent in yeast actin.  Archives of Biochemistry and Biophysics.  1999 October. 370(1):105-11.
[PubMed]

Eads J, Mahoney N, Vorobiev S, Bresnick A, Wen K, Rubenstein P, Haarer B, Almo S.  Structure determination and characterization of Saccharomyces cerevisiae profilin.  Biochemistry.  1998 August. 37(32):11171-81.
[PubMed]

Rubenstein P, Tobacman L.  Highlights of the Molecular Interactions of Actin meeting, Hawaii, 1-5 April 1997.  Journal of Muscle Research and Cell Motility.  1998 April. 19(3):271-6.
[PubMed]

Orlova A, Chen X, Rubenstein P, Egelman E.  Modulation of yeast F-actin structure by a mutation in the nucleotide-binding cleft.  Journal of Molecular Biology.  1997 August. 271(2):235-43.
[PubMed]

Feng L, Kim E, Lee W, Miller C, Kuang B, Reisler E, Rubenstein P.  Fluorescence probing of yeast actin subdomain 3/4 hydrophobic loop 262-274. Actin-actin and actin-myosin interactions in actin filaments.  The Journal of Biological Chemistry.  1997 July. 272(27):16829-37.
[PubMed]

Kuang B, Rubenstein P.  The effects of severely decreased hydrophobicity in a subdomain 3/4 loop on the dynamics and stability of yeast G-actin.  The Journal of Biological Chemistry.  1997 February. 272(7):4412-8.
[PubMed]

Kuang B, Rubenstein P.  Beryllium fluoride and phalloidin restore polymerizability of a mutant yeast actin (V266G,L267G) with severely decreased hydrophobicity in a subdomain 3/4 loop.  The Journal of Biological Chemistry.  1997 January. 272(2):1237-47.
[PubMed]

Miller C, Wong W, Bobkova E, Rubenstein P, Reisler E.  Mutational analysis of the role of the N terminus of actin in actomyosin interactions. Comparison with other mutant actins and implications for the cross-bridge cycle.  Biochemistry.  1996 December. 35(51):16557-65.
[PubMed]

Chen X, Rubenstein P.  A mutation in an ATP-binding loop of Saccharomyces cerevisiae actin (S14A) causes a temperature-sensitive phenotype in vivo and in vitro.  The Journal of Biological Chemistry.  1995 May. 270(19):11406-14.
[PubMed]

Chen X, Peng J, Pedram M, Swenson C, Rubenstein P.  The effect of the S14A mutation on the conformation and thermostability of Saccharomyces cerevisiae G-actin and its interaction with adenine nucleotides.  The Journal of Biological Chemistry.  1995 May. 270(19):11415-23.
[PubMed]

Crosbie R, Miller C, Chalovich J, Rubenstein P, Reisler E.  Caldesmon, N-terminal yeast actin mutants, and the regulation of actomyosin interactions.  Biochemistry.  1994 March. 33(11):3210-6.
[PubMed]

Chen X, Cook R, Rubenstein P.  Yeast actin with a mutation in the "hydrophobic plug" between subdomains 3 and 4 (L266D) displays a cold-sensitive polymerization defect.  The Journal of Cell Biology.  1993 December. 123(5):1185-95.
[PubMed]

Cook R, Root D, Miller C, Reisler E, Rubenstein P.  Enhanced stimulation of myosin subfragment 1 ATPase activity by addition of negatively charged residues to the yeast actin NH2 terminus.  The Journal of Biological Chemistry.  1993 February. 268(4):2410-5.
[PubMed]

Babcock G, Rubenstein P.  Control of profilin and actin expression in muscle and nonmuscle cells.  Cell Motility and the Cytoskeleton.  1993. 24(3):179-88.
[PubMed]

Sheff D, Rubenstein P.  Isolation and characterization of the rat liver actin N-acetylaminopeptidase.  The Journal of Biological Chemistry.  1992 October. 267(28):20217-24.
[PubMed]

Cao L, Babcock G, Rubenstein P, Wang Y.  Effects of profilin and profilactin on actin structure and function in living cells.  The Journal of Cell Biology.  1992 June. 117(5):1023-9.
[PubMed]

Wang Y, Rubenstein P.  Splicing of two alternative exon pairs in beta-tropomyosin pre-mRNA is independently controlled during myogenesis.  The Journal of Biological Chemistry.  1992 June. 267(17):12004-10.
[PubMed]

Cook R, Blake W, Rubenstein P.  Removal of the amino-terminal acidic residues of yeast actin. Studies in vitro and in vivo.  The Journal of Biological Chemistry.  1992 May. 267(13):9430-6.
[PubMed]

Sheff D, Rubenstein P.  Amino-terminal processing of actins mutagenized at the Cys-1 residue.  The Journal of Biological Chemistry.  1992 February. 267(4):2671-8.
[PubMed]

Wang Y, Rubenstein P.  Choice of 3' cleavage/polyadenylation site in beta-tropomyosin RNA processing is differentiation-dependent in mouse BC3H1 muscle cells.  The Journal of Biological Chemistry.  1992 February. 267(4):2728-36.
[PubMed]

Cook R, Rubenstein P.  The genertion and isolation of mutant actins in practical approaches in cell biology.  IRL Press.  1992. 

Cook R, Sheff D, Rubenstein P.  Unusual metabolism of the yeast actin amino terminus.  The Journal of Biological Chemistry.  1991 September. 266(25):16825-33.
[PubMed]

Rubenstein P.  The functional importance of multiple actin isoforms.  BioEssays : news and reviews in molecular, cellular and developmental biology.  1990 July. 12(7):309-15.
[PubMed]

Sheff D, Rubenstein P.  Identification of N-acetylmethionine as the product released during the NH2-terminal processing of a pseudo-class I actin.  The Journal of Biological Chemistry.  1989 July. 264(19):11491-6.
[PubMed]

Rubenstein P, Solomon L, Solomon T, Gay L.  Actin structure-function relationships in vitro using oligodeoxynucleotide-directed site-specific mutagenesis.  Cell Motility and the Cytoskeleton.  1989. 14(1):35-9.
[PubMed]

Shires A, Rubenstein P.  Nonuniform behavior of multiple isoactins in the same cell is a cell-dependent phenomenon.  Cell Motility and the Cytoskeleton.  1989. 14(2):263-70.
[PubMed]

Babcock G, Rubenstein P.  Synthesis of mammalian profilin in Escherichia coli and its characterization.  Cell Motility and the Cytoskeleton.  1989. 14(2):230-6.
[PubMed]

Solomon T, Solomon L, Gay L, Rubenstein P.  Studies on the role of actin's aspartic acid 3 and aspartic acid 11 using oligodeoxynucleotide-directed site-specific mutagenesis.  The Journal of Biological Chemistry.  1988 December. 263(36):19662-9.
[PubMed]

Wang Y, Rubenstein P.  Epidermal growth factor controls smooth muscle alpha-isoactin expression in BC3H1 cells.  The Journal of Cell Biology.  1988 March. 106(3):797-803.
[PubMed]

Rubenstein P, Solomon L, Martin D.  Specificity of the actin amino terminal processing protease.  in Molecular Bology of Intracellular Protein Sorting and Organelle Assembly., Alan R. Liss, Inc. (New York).  1988. 

Solomon L, Rubenstein P.  Studies on the role of actin's N tau-methylhistidine using oligodeoxynucleotide-directed site-specific mutagenesis.  The Journal of Biological Chemistry.  1987 August. 262(23):11382-8.
[PubMed]

Martin D, Rubenstein P.  Alternate pathways for removal of the class II actin initiator methionine.  The Journal of Biological Chemistry.  1987 May. 262(13):6350-6.
[PubMed]

Rubenstein P, Redman K, Solomon L, Martin D.  Amino-terminal processing of Dictyostelium discoideum actin.  Methods in Cell Biology.  1987. 28:231-43.
[PubMed]

Rubenstein P, Redman K, Solomon L.  Amino terminal processing of actin.  Microbiology.  1986. 

Strauch A, Offord J, Chalkley R, Rubenstein P.  Characterization of actin mRNA levels during BC3H1 cell differentiation.  The Journal of Biological Chemistry.  1986 January. 261(2):849-55.
[PubMed]

Redman K, Martin D, Korn E, Rubenstein P.  Lack of NH2-terminal processing of actin from Acanthamoeba castellanii.  The Journal of Biological Chemistry.  1985 November. 260(27):14857-61.
[PubMed]

Solomon L, Rubenstein P.  Correct NH2-terminal processing of cardiac muscle alpha-isoactin (class II) in a nonmuscle mouse cell.  The Journal of Biological Chemistry.  1985 June. 260(12):7659-64.
[PubMed]

Strauch A, Rubenstein P.  A vascular smooth muscle alpha-isoactin biosynthetic intermediate in BC3H1 cells. Identification of acetylcysteine at the NH2 terminus.  The Journal of Biological Chemistry.  1984 June. 259(11):7224-9.
[PubMed]

Moore S, Strauch A, Yoder E, Rubenstein P, Hart M.  Cerebral microvascular smooth muscle in tissue culture.  In Vitro.  1984 June. 20(6):512-20.
[PubMed]

Strauch A, Rubenstein P.  Induction of vascular smooth muscle alpha-isoactin expression in BC3H1 cells.  The Journal of Biological Chemistry.  1984 March. 259(5):3152-9.
[PubMed]

Redman K, Rubenstein P.  Actin amino-terminal acetylation and processing in a rabbit reticulocyte lysate.  Methods in Enzymology.  1984. 106:179-92.
[PubMed]

Cass K, Clark E, Rubenstein P.  Is the onset of actin histidine methylation under development control in the chick embryo.  Archives of Biochemistry and Biophysics.  1983 September. 225(2):731-9.
[PubMed]

Rubenstein P, Martin D.  NH2-terminal processing of Drosophila melanogaster actin. Sequential removal of two amino acids.  The Journal of Biological Chemistry.  1983 September. 258(18):11354-60.
[PubMed]

Rubenstein P, Martin D.  NH2-terminal processing of actin in mouse L-cells in vivo.  The Journal of Biological Chemistry.  1983 March. 258(6):3961-6.
[PubMed]

Rubenstein P.  Applications of polyacrylamide gel electrofocusing and electrophoresis to contractile and cytoskeletal protein research.  Electrophoresis, Elsevier Pub. (Amsterdam).  1983. 

Rubenstein P, Ruppert T, Sandra A.  Selective isoactin release from cultured embryonic quail breast muscle cells.  The Journal of Biological Chemistry.  1982. 92:164-9.

Redman K, Rubenstein P.  NH2-terminal processing of Dictyostelium discoideum actin in vitro.  The Journal of Biological Chemistry.  1981 December. 256(24):13226-9.
[PubMed]

Rubenstein P.  Differential behavior of gizzard isoactins.  Archives of Biochemistry and Biophysics.  1981 September. 210(2):598-608.
[PubMed]

Ionasescu V, Braga S, Kaeding L, Rubenstein P, Kalnitsky G, Chatterjee R.  Muscle ribosome detachment factor - A role in the pathogenesis of Duchenne muscular dystrophy?.  Acta Neurology Scand….  1981. 64:108-21.

Rubenstein P, Deuchler J, Redman K, Smith P.  NH2-Therminal acetylation of D. discoideum actin in a cell-free protein synthesizing system.  The Journal of Biological Chemistry.  1981. 256:8149-8155.

Rubenstein P, Dryer R.  S-Acetonyl-CoA: A nonreactive analog of acetyl-CoA.  The Journal of Biological Chemistry.  1980. 255:7858-62.

Rubenstein P, Ivarie R.  Isolation of two different molecular weight polypeptides copurifying with rat liver tyrosine aminotransferase.  Archives of Biochemistry and Biophysics.  1979 April. 194(1):299-311.
[PubMed]

Rubenstein P, Deuchler J.  Acetylated and nonacetylated actins in Dictyostelium discoideum.  The Journal of Biological Chemistry.  1979. 254:11142-47.

Rubenstein P, Sealy L, Marshall S, Chalkley R.  Cellular protein synthesis and inhibition of cell division appear to be independent of butyrate induced histone hyperacetylation.  Nature.  1979. 280:692-693.

Ionasescu V, Stern L, Ionasescu R, Rubenstein P.  Stimulatory effects of drugs for protein synthesis on muscle cell cultures in Duchenne dystrophy.  Annals of Neurology.  1979. 5:107-111.

Levinson W, Oppermann H, Rubenstein P, Jackson J.  Host range restriction of vesicular stomatitis virus on duck embryo cells.  Journal of Virology.  1978. 85:612-7.

Spudich J, Mockrin S, Brown S, Rubenstein P, Levinson W.  The organization and interaction of actin and myosin in nonmuscle cells.  Progress in Clinical and Biological Research.  1977. 17:545-58.
[PubMed]

Rubenstein P, Spudich J.  Actin microheterogeneity in chick embryo fibroblasts.  Proceedings of the National Academy of Sciences of the United States of America.  1977 January. 74(1):120-3.
[PubMed]

Rubenstein P, Strominger J.  Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexoses. 8. Mechanistic roles of enzyme E-1 and pyridoxamine 5'-phosphate in the formation of cytidine diphosphate-4-keto-3,6-dideoxy-D-glucose from cytidine diphosphate-4-keto-6-deoxy-D-glucose.  The Journal of Biological Chemistry.  1974 June. 249(12):3776-81.
[PubMed]

Rubenstein P, Strominger J.  Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexoses. 8. Studies of the properties of E3 and its role in the formation of cytidine diphosphate-4-keto-3,6-dideoxyglucose.  The Journal of Biological Chemistry.  1974 June. 249(12):3782-88.
[PubMed]

Rubenstein P, Strominger J.  Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexosis. IX. Purification and properties of the cytidine diphosphate-D-glucose pyrophosphorylase from Pasteurella pseudotuberculosis. type V.  The Journal of Biological Chemistry.  1974 June. 249(12):3789-96.
[PubMed]

Stevens C, Schultze K, Smith D, Pillai P, Rubenstein P, Strominger J.  Synthesis of 3,6-dideoxy-D-erythro-hexos-4-ulose and identification as the 3,6-dideoxy-4-ketohexose from Pasteurella pseudotuberculosis.  Journal of American Chemical Society.  1973. 95:5767-68.

Wickus G, Rubenstein P, Warth A, Strominger J.  Partial purification and some properties of the uridine diphospho-N-acetylglucosamine-enolpyruvate reductase from Staphylococcus epidermidis.  Journal of Bacteriology.  1973 January. 113(1):291-4.
[PubMed]

Streitwieser A, J, Hollyhead W, Pudjaatmaka A, Owens P, Kruger T, Rubenstein P, MacQuarrie R, Brokaw M, Chu W, Niemeyer H.  The Bronsted correlation and hydrogen isotope exchange kinetics of fluorenes, benzfluorenes, and indene with methanolic sodium methoxide.  Journal of American Chemical Society.  1971. 93:5088-96.

Gibian M, Rubenstein P, Ficklin W.  The formation and activities of substituted phenacylchymotrypsins.  Biochimica et Biophysica Acta.  1969. 191(3):677-85.
[PubMed]

Date Last Modified: 07/30/2014 - 12:59:28