Division Director, Pediatric Critical Care
Interdisciplinary Graduate Program in Molecular and Cellular Biology, Faculty Mentor
Director, Pediatric Critical Care Fellowship Program
Medical Scientist Training Program, Faculty Mentor
Associate Professor of Pediatrics
- Critical Care
Contact Information
Office: 7770-G JPP
Iowa City, IA 52242
Office Phone: 319-384-8545
Email: jessica-moreland@uiowa.edu
Web: Moreland Laboratory
Education
BS, Zoology, Duke University, Durham, North Carolina
MD, Medicine, Vanderbilt University, Nashville, TN
Residency, Pediatrics, Vanderbilt University, Nashville, TN
Fellowship, Pediatric Critical Care, Vanderbilt University, Nashville, TN
Licensure and Certifications
Iowa Medical License, Iowa Board of Medical Examiners
Pediatric Critical Care Board Certification, American Board of Pediatrics
Education/Training Program Affiliations
Biosciences Graduate Program
Interdisciplinary Graduate Program in Molecular and Cellular Biology
Medical Scientist Training Program
Research Summary
The primary focus of our laboratory is to understand the role of the NADPH oxidase and Nox-derived reactive oxygen species (ROS) in intracellular signaling in neutrophils. We are interested in oxidant signaling both under resting/basal and stimulated conditions. Specifically, we are exploring the role of ROS signaling during neutrophil priming. Neutrophil priming is an intermediate state of cell activation characterized by enhanced responsiveness to subsequent stimuli. We are particularly interested in primed states generated in response to inflammatory and infectious stimuli (including TNF-a and endotoxin) as might occur during Gram-negative sepsis. As a component of this project, we are characterizing the role of the anion transporter ClC-3 in regulation of the NADPH oxidase. This interest developed after ClC-3 knockout mice were found to have a defect in innate immunity and later demonstrated to have multiple abnormalities in neutrophil function.
A second ongoing project in the laboratory focuses on understanding the early components of the innate immune response to bacterial pathogens in the lung. We are specifically interested in dissecting neutrophil-endothelial cell interactions, and understanding the determinants of neutrophil transendothelial migration. These studies focus on two very distinct pulmonary pathogens: Streptococcus pneumoniae, the leading cause of community acquired pneumonia, and Francisella tularensis, a potential agent for bioterrorism. We have developed an in vitro model of neutrophil transmigration that includes stimulation of primary human pulmonary endothelial cells with live bacteria, followed by the addition of human PMNs. For our studies of Francisella tularensis, we are also exploring interactions between this pathogen and alveolar epithelial cells.
Center, Program and Institute Affiliations
Center for Immunology and Immune-based Diseases
Inflammation Program
Selected Publications
Show All
Endotoxin priming of neutrophils requires endocytosis and NADPH oxidase-dependent endosomal reactive oxygen species..
Lamb F,
Hook J,
Hilken B,
Huber J,
Volk A,
Moreland J.
Journal of Biological Chemistry.
2012
January; 287
(15)
:12395-404.
[Link]
Priming of Neutrophils and Differentiated PLB-985 Cells by Pathophysiological Concentrations of TNF-alpha Is Partially Oxygen Dependent..
Volk A,
Barber B,
Goss K,
Ruff J,
Heise C,
Hook J,
Moreland J.
J Innate Immunity.
2011
April; 3
(3)
:298-314.
[Link]
Activation of IClswell by TNF-α requires CIC-3-dependent endosomal reactive oxygen production..
Matsuda J,
Filali M,
Moreland J,
Miller F,
Lamb F.
Journal of Biological Chemistry.
2010
July; 285
(30)
:22864-73.
[Link]
ClC-3 and ICLswell are required for normal neutrophil chemotaxis and shape change.
Volk A,
Heise C,
Hougen J,
Artman C,
Volk K,
Wessels D,
SOLL D,
Nauseef W,
Lamb F,
Moreland J.
J Biol Chem.
2008
October; 283
(49)
:34315-26.
[Link]
Endotoxin priming of neutrophils requires NADPH-oxidase generated oxidants and is regulated by the anion transporter ClC-3.
Moreland J,
Volk A,
Matsuda J,
Hook J,
Bailey G,
Nauseef W,
Lamb F.
Journal of Biological Chemistry.
2007
November; 282
(47)
:33958-67.
[Link]
Overexpression of CLC-3 in HEK293T cells yields novel currents that are pH dependent.
Matsuda J,
Filali M,
Volk K,
Collins M,
Moreland J,
Lamb F.
Am J Physiol Cell Physiol.
2007
October; 294
:C251-C262.
[Link]
Date Last Modified: 05/28/2013 -
11:28:07