Pathology

J. Stacey Klutts, MD, PhD

Portrait

Assistant Professor of Pathology  - Clinical Pathology

Contact Information

Primary Office: BW23 Veterans Affairs Medical Center
Iowa City, IA 52242
Primary Office Phone: 319-338-0581-5530

Office: 113 Veterans Affairs Medical Center
Iowa City, IA 52242

Email: stacey-klutts@uiowa.edu
Web: Dr. Klutts Research Laboratory

Education

BS, Chemistry, Missouri State University, Springfield, MO
MD, University of Arkansas for Medical Sciences, Little Rock, AR
PhD, University of Arkansas for Medical Sciences, Little Rock, AR

Residency, Washington University School of Medicine/Barnes-Jewish Hospital, St. Louis, MO
Post Doctoral, Washington University School of Medicine, St. Louis, MO

Licensure and Certifications

Diplomate, Clinical Pathology, American Board of Pathology

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Pathology Graduate Program
Interdisciplinary Graduate Program in Molecular and Cellular Biology

Research Summary

Research in my laboratory primarily focuses on the human pathogenic fungus Aspergillus fumigatus. This is a ubiquitous environmental mold that can cause a variety of diseases in humans, including a life-threatening invasive infection in the setting of immune compromise. Treatment options exist for invasive aspergillosis, but therapeutic failures are common with mortality rates as high as 85% in some patient populations. Currently, the main focus of the laboratory is to better understand the process of cell wall synthesis in A. fumigatus. Pathogenicity and survival of A. fumigatus in vivo requires a number of virulence factors, with the ability to generate a rigid cell wall being one of the most important. Targeting cell wall synthesis with anti-fungal therapy has been successful in the treatment of a number of fungal infections. However, identification of additional drug targets in the pathway of cell wall assembly is hindered by our limited knowledge of these synthetic mechanisms. To study cell wall synthesis, we are taking three complementary approaches. One aim is to develop enzymatic assays for monitoring the activity of glycosyltransferase enzymes likely involved in cell wall synthesis and to use these assays to purify/identify the corresponding proteins. We are also taking a forward genetic approach with the development of a mutagenesis library in A. fumigatus that will be screened for strains with cell wall defects or with the biochemical assays discussed above. Lastly, we are systematically deleting genes in A. fumigatus that we hypothesize are involved in cell wall synthesis and are studying the cell wall structures and phenotypes of these knockout strains in detail.

All Publications

Schlichting J, Mengeling M, Makki N, Malhotra A, Halfdanarson T, Klutts J.  Increasing Colorectal Cancer Screening an an Overdue Population: Participation and Cost Impacts of Adding Telephone Calls to a FIT Mailing Program.  Journal of Community Health.  2014 February 6. 39(2):239-47.
[PubMed]

Charlton M, Mengeling M, Halfdanarson T, Makki N, Malhotra A, Klutts S, Levy B, Kaboli P.  Evaluation of a Home-Based Colorectal Cancer Screening Intervention in a Rural State.  Journal of Rural Health.  2013 October 25.  30(3):322-32.
[PubMed]

Kieler J, Duong K, Moye-Rowley W, Klutts J.  Targeted Gene Deletion in Aspergillus fumigatus Using Microbial Machinery and a Recyclable Marker. doi:pii: S0167-7012(13)00309-6..  J Microbiol Methods.  2013 October 23. 95(3):373-8.
[PubMed]

Gailey M, Klutts J, Jensen C.  Fine needle aspiration of histoplasmosis in the era of endoscopic ultrasound (EUS) and endobronchial ultrasound (EBUS).  Can Cytopath.  2013 May 15. 121(9):508-17.
[PubMed]

Baselski V, Klutts J.  Point-Counterpoint: Quantitative cultures of bronchoscopically obtained specimens should be performed for optimal management in patients with ventilator associated pneumonia.  Journal of clinical microbiology.  2013 March. 51(3):740-44.
[PubMed]

Heiss C, Skowyra M, Liu H, Klutts J, Wang Z, Williams M, Srikanta D, Beverly S, Azadi P, Doering T.  Unusual galactofuranose modification of a capsule polysaccharide in the pathogenic yeast Cryptococcus neoformans.  J. Biol. Chem..  2013. 288:10994-1003.

Paul S, Klutts J, Moye-Rowley W.  Analysis of Promoter Function in Aspergillus fumigatus.  Eukaryotic cell.  2012 September. 11(9):1167-77.
[PubMed]

Kieler J, Duong K, Klutts J.  Amylases and Cell Wall Synthesis in Aspergillus fumigatus.  Keystone Conference: Fungal Pathogens: From Basic Biology to Drug Discovery. Santa Fe, NM.  2012. 

Klutts J, Robinson-Dunn B.  A Critical Appraisal of the Role of the Clinical Microbiology Laboratory in Diagnosis of Invasive Fungal Infections.  Journal of clinical microbiology.  2011 September. 49(9):S39-S42.

Samar D, Mecklenburg E, Kieler J, Klutts J.  Agrobacterium Mediated Transformation and Splinkerette PCR for the Development and Characterization of an Aspergillus fumigatus Cell Wall Mutant Library.  American Society of Microbiology General Meeting. New Orleans, LA.  2011. 

Duong K, Klutts J.  The Role of Amylases in Aspergillus fumigatus cell wall synthesis.  American Society of Microbiology General Meeting - San Diego, CA.  2010. 

Mecklenburg E, Klutts J.  Utilization of a Novel Transformation and Screening Protocol to Generate an Aspergillus fumigatus Cell Wall Mutant Library.  American Society of Microbiology General Meeting - San Diego, CA.  2010. 

Reilly M, Levery S, Castle S, Klutts J, Doering T.  A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans.  The Journal of biological chemistry.  2009 December. 284(52):36118-27.
[PubMed]

Klutts J, Ford B, Perez N, Gronowski A.  Evidence-based approach for interpretation of Epstein-Barr virus serological patterns.  Journal of clinical microbiology.  2009 October. 47(10):3204-10.
[PubMed]

Heiss C, Klutts J, Wang Z, Doering T, Azadi P.  The structure of Cryptococcus neoformans galactoxylomannan contains beta-D-glucuronic acid.  Carbohydrate research.  2009 May. 344(7):915-20.
[PubMed]

Kieler J, Klutts J.  A Glucosyltransferase in Aspergillus fumigatus with a Potential Role in Cell Wall Synthesis.  American Society of Microbiology General Meeting.  2009. 

Castle S, Owuor E, Thompson S, Garnsey M, Klutts J, Doering T, Levery S.  Beta1,2-xylosyltransferase Cxt1p is solely responsible for xylose incorporation into Cryptococcus neoformans glycosphingolipids.  Eukaryotic cell.  2008 September. 7(9):1611-5.
[PubMed]

Klutts J, Wu A, Smith A, Yen-Lieberman B, Gronowski A.  Diagnostic performance of a new automated heterophile antibody test in adults and children.  Diagnostic microbiology and infectious disease.  2008 July. 61(3):351-3.
[PubMed]

Klutts J, Doering T.  Cryptococcal xylosyltransferase 1 (Cxt1p) from Cryptococcus neoformans plays a direct role in the synthesis of capsule polysaccharides.  The Journal of biological chemistry.  2008 May. 283(21):14327-34.
[PubMed]

Kieler J, Klutts J.  A Novel Glucosyltransferase in Aspergillus fumigatus—New Implications for Cell Wall Synthesis.  Interscience Conference on Antimicrobial Agents and Chemotherapy/Infectious Diseases Society of America. Washington, D.C.  2008. 

Klutts J, Levery S, Doering T.  A beta-1,2-xylosyltransferase from Cryptococcus neoformans defines a new family of glycosyltransferases.  The Journal of biological chemistry.  2007 June. 282(24):17890-9.
[PubMed]

Klutts J, Scott M.  Disorders of Water, Electrolyte, and Acid-Base Metabolism.  Elsevier, Philadelphia, PA.  2007. 

Scott M, LeGrys V, Klutts J.  Electrolytes and Blood Gases.  Elsevier, Philadelphia, PA.  2007. 

Klutts J.  Hot, Tired and Lump - A Case of Classical EBV-Related Mononucleosis.  John Wiley & Sons, Inc., Hoboken, NH.  2007. 

Klutts J, Yoneda A, Reilly M, Bose I, Doering T.  Glycosyltransferases and their products: cryptococcal variations on fungal themes.  FEMS yeast research.  2006 June. 6(4):499-512.
[PubMed]

Klutts J, Levery S, Doering T.  Xylosyltransferases and Capsule Biosynthesis in Cryptococcus neoformans.  Academy of Clinical Laboratory Physicians and Scientists (ACLPS) Annual Meeting. Chicago, IL.  2006. 

Klutts J, Levery S, Doering T.  Xylosyltransferases and Capsule Biosynthesis in Cryptococcus neoformans.  American Society for Microbiology, General Meeting. Orlando, FL.  2006. 

Klutts J, Levery S, Doering T.  A Beta-1,2 Xylosyltransferase from Cryptococcus neoformans.  ACLP Annual Meeting, Pittsburg, PA.  2005. 

Klutts J, Levery S, Doering T.  A Beta-1,2 Xylosyltransferase from Cryptococcus neoformans.  American Society for Microbiology General Meeting. Atlanta, GA.  2005. 

Klutts J, Levery S, Doering T.  A Beta-1,2 Xylosyltransferase from Cryptococcus neoformans.  Annual Meeting of the Society for Glycobiology. Boston, MA.  2005. 

Klutts J, Levery S, Doering T.  A Beta-1,2 Xylosyltransferase from Cryptococcus neoformans.  Gordon Conference on Glycobiology. Ventura, VA.  2005. 

Klutts J, Levery S, Doering T.  A Beta-1,2 Xylosyltransferase from Cryptococcus neoformans.  International Meeting of Cryptococcus and Cryptococcosis (ICCC), Biennial Meeting. Boston, MA .  2005. 

Scott M, LeGrys V, Klutts J.  Electrolytes and Blood Gases.  Elsevier, Philadelphia, PA.  2005. 

Klutts J, Scott M.  Physiology and Disorders of Water, Electrolytes, and Acid-Base Metabolism.  Elsevier, Philadelphia, PA.  2005. 

Griffith C, Klutts J, Zhang L, Levery S, Doering T.  UDP-glucose dehydrogenase plays multiple roles in the biology of the pathogenic fungus Cryptococcus neoformans.  The Journal of biological chemistry.  2004 December. 279(49):51669-76.
[PubMed]

Klutts J, Liao R, Dunne W, Gronowski A.  Evaluation of a multiplexed bead assay for assessment of Epstein-Barr virus immunologic status.  Journal of clinical microbiology.  2004 November. 42(11):4996-5000.
[PubMed]

Klutts J, Liao R, Dunne W, Gronowski A.  Evaluation of the Bio-Plex 2200 Multi-Analyte System for Detection of Antibodies to Epstein-Barr Virus.  ACLPS Annual Meeting. Denver, CO.  2004. 

Klutts J, Doering T.  Purification and Characterization of a Xylosyltransferase from Cryptococcus neoformans.  American Society for Microbiology, General Meeting. New Orleans, LA.  2004. 

Klutts J, Dunne W.  Detection of Gram-Positive and Gram-Negative Bacteria in Platelet Products Using an Acridine Orange Staining Technique.  ACLPS Annual Meeting. Tucson, AZ.  2003. 

Klutts J, Eby C.  Evaluation of a Micro-particle Agglutination Assay for the Diagnosis of Heparin-Induced Thrombocytopenia.  ACLPS Annual Meeting. Tucson, AZ.  2003. 

Klutts J, Pastuszak I, Edavana V, Thampi P, Pan Y, Abraham E, Carroll J, Elbein A.  Purification, cloning, expression, and properties of mycobacterial trehalose-phosphate phosphatase.  The Journal of biological chemistry.  2003 January. 278(4):2093-100.
[PubMed]

Klutts J, Hatanaka K, Pan Y, Elbein A.  Biosynthesis of d-arabinose in Mycobacterium smegmatis: specific labeling from d-glucose.  Archives of biochemistry and biophysics.  2002 February. 398(2):229-39.
[PubMed]

Klutts J.  The Biosynthesis of D-Arabinose and D-Trehalose in Mycobacterium smegmatis, PhD Dissertation-University of Arkansas for Medical Sciences.  2002. 

Klutts J, Pan Y, Elbein A.  The Purification and characterization of a Trehalose-phosphate Phosphatase from Mycobacterium smegmatis.  ACLPS Annual Meeting. New York, NY.  2002. 

Samar D, Kieler J, Klutts J.  Identification and Analysis of the Single Beta-1,3:1,4 Mixed Linkage Glucan Synthase Involved in Cell Wall Synthesis of Aspergillus fumigatus (In Preparation for Submission). 

Date Last Modified: 08/27/2014 - 11:27:51