Pathology

Aaron D. Bossler, MD, PhD

Portrait

Director, Molecular Pathology Laboratory
Director, Molecular Genetic Pathology Fellowship
Co-Director, Resident Rotation in Molecular Pathology
Clinical Associate Professor of Pathology  - Clinical Pathology

Contact Information

Primary Office: C606 General Hospital
Iowa City, IA 52242
Primary Office Phone: 319-384-9566

Email: aaron-bossler@uiowa.edu

Education

BA, Biochemistry, Columbia University, New York City, NY
MD, University of Iowa Carver College of Medicine, Iowa City, IA
PhD, Molecular Biology, University of Iowa Carver College of Medicine, Iowa City, IA

Residency, Clinical Pathology, University of Iowa Carver College of Medicine, Iowa City, IA
Fellowship, Molecular Genetic Pathology, Hospital of the University of Pennsylvania, Philadelphia, PA

Licensure and Certifications

Diplomat in Molecular Genetic Pathology, American Board of Pathology
Diplomat in Clinical Pathology, American Board of Pathology

Research Summary

Dr. Bossler's clinical service duties include directing the molecular pathology laboratory, developing and validating new, rapid, and cost effective molecular assays for genetic, neoplastic and infectious disease testing, and interpreting and reporting patient results from clinical molecular testing. His research interest is in understanding the mechanisms and pathways which lead to oncogenesis particularly regarding the association of infection with high risk human papillomavirus (HPV) and the development of squamous cell cancer.

All Publications

Ma D, Stence A, Bossler A, Hackman J, Bellizzi A.  Identification of KIT activating mutations in paediatric solitary mastocytoma.  Histopathology.  2013 June 24. 
[PubMed]

Ma D, Stence A, Bossler A, Hackman J, Bellizzi A.  Identification of KIT activating mutations in paediatric solitary myastocytoma..  2013 June 24. 
[PubMed]

Bossler A.  Implementation of New Molecular Pathology CPT Codes by CMS.  Association for Molecular Pathology Annual Meeting.  2013. 

Marko D, Saffert R, Cunningham S, Hyman J, Walsh J, Arbefeville S, Howard W, Pruessner J, Safwat N, Cockerill F, Bossler A, Patel R, Richter S.  Evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting gram-negative bacilli isolated from cultures from cystic fibrosis patients.  Journal of clinical microbiology.  2012 June. 50(6):2034-9.
[PubMed]

Ma D, Caponetti G, Hackman J, Heusel J, Bossler A.  Evaluation of Multiplex Quantitative Measurement of BCR-ABL1 Using the International Reporting Scale.  Association for Molecular Pathology Annual Meeting, Long Beach, CA.  2012. 

Ma D, Caponetti G, Heusel J, Bossler A.  Identification of c-KIT Activating Mutation in Pediatric Isolated Mastocytoma.  Association for Molecular Pathology Annual Meeting, Long Beach, CA.  2012. 

Ma D, Caponetti G, Heusel J, Bossler A.  Identification of c-KIT Activating Mutation in Pediatric Isolated Mastocytoma.  Association for Molecular Pathology Annual Meeting, Long Beach, CA.  2012. 

Caponetti G, Racila E, Ma D, Heusel J, Bossler A.  Improved Detection of the BRAF c.1799T>A (p.V600E) Mutation in Melanoma with a Single Nucleotide Primer Extension Assay.  United States and Canadian Academy of Pathology’s 101st Annual Meeting, Vancouver, Canada.  2012. 

Savage J, Ma D, Caponetti G, Bossler A, Heusel J.  Microsatellite Instability Discordance in Synchronous Colorectal Adenocarcinoma-Evidence for Parallel Oncogenic Pathways Involving Distinct Molecular Mechanisms.  Association for Molecular Pathology Annual Meeting, Long Beach, CA.  2012. 

Bossler A, Caliendo A.  Molecular Methods in Diagnosis and Monitoring of Infectious Diseases.  Elsevier, New York.  2012. 

Gunsolly C, Jordan J, Bossler A.  Performance of the cobas® 4800 HPV Test on SurePath Cytology Specimens.  Association for Molecular Pathology Annual Meeting, Long Beach, CA.  2012. 

Bossler A, Gunsolly C, Pyne M, Rendo A, Rachel J, Mills R, Miller M, Sipley J, Hillyard D, Jenkins S, Essmyer C, Young S, Lewinski M, Rennert H.  Performance of the COBAS® AmpliPrep/COBAS TaqMan® automated system for hepatitis C virus (HCV) quantification in a multi-center comparison.  Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology.  2011 February. 50(2):100-3.
[PubMed]

Arbefeville S, Bossler A.  Bronchiolitis in an Infant; Infection with Human Metapneumovirus.  Springer-Verlag, Berlin Heidelberg.  2011. 

Gunsolly C, Bossler A.  cobas®4800 Versus Cervista® HPV Test Performance on SurePath™ Cytology Specimens.  27th International Papillomavirus Conference, Berlin.  2011. 

Bossler A, Arbefeville S, Stence A, Pruessner J, Heusel J.  Comparison of somatic point mutation detection methods in colorectal carcinoma; addressing the challenges of analytical sensitivity.  Association for Molecular Pathology, Annual Meeting, Dallas, TX.  2011. 

Marko D, Saffert R, Cunningham S, Hyman J, Walsh J, Arbefeville S, Howard W, Pruessner J, Safwat N, Cockerill F, Bossler A, Patel R, Richter S.  Evaluation of the Bruker Biotyper and Axima@Saramis Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Systems for the Identification of Non-Fermenting Gram Negative Rods (NFGNR) From Cystic Fibrosis (CF) Patients.  American Society of Microbiology Annual Meeting.  2011. 

Arbefeville S, Bossler A.  Human Papillomavirus Infection and ASC-US Triage.  Springer-Verlag, Berlin Heidelberg.  2011. 

Tsumagari K, Chen D, Hackman J, Bossler A, Ehrlich M.  FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease.  Journal of medical genetics.  2010 November. 47(11):745-51.
[PubMed]

Shenoy A, Markowitz J, Bonnemann C, Krishnamoorthy K, Bossler A, Tseng B.  Muscle-Eye-Brain disease.  Journal of clinical neuromuscular disease.  2010 March. 11(3):124-6.
[PubMed]

Harris G, Strand G, Gunsolly C, Lee J, Bossler A.  PTPN13 expression correlates with survival in HPV-positive HNSCC.  American Academy of Otolaryngology Head and Neck Surgery Foundation Annual Meeting, Boston, MA.  2010. 

Lace M, Anson J, Klingelhutz A, Lee J, Bossler A, Haugen T, Turek L.  Human papillomavirus (HPV) type 18 induces extended growth in primary human cervical, tonsillar, or foreskin keratinocytes more effectively than other high-risk mucosal HPVs.  Journal of virology.  2009 November. 83(22):11784-94.
[PubMed]

Hoover A, Strand G, Nowicki P, Anderson M, Vermeer P, Klingelhutz A, Bossler A, Pottala J, Hendriks W, Lee J.  Impaired PTPN13 phosphatase activity in spontaneous or HPV-induced squamous cell carcinomas potentiates oncogene signaling through the MAP kinase pathway.  Oncogene.  2009 November. 28(45):3960-70.
[PubMed]

Lace M, Anson J, Klingelhutz A, Harada H, Taniguchi T, Bossler A, Haugen T, Turek L.  Interferon-beta treatment increases human papillomavirus early gene transcription and viral plasmid genome replication by activating interferon regulatory factor (IRF)-1.  Carcinogenesis.  2009 August. 30(8):1336-44.
[PubMed]

Arbefeville S, Junkins A, Nasr M, Richter S, Bossler A.  Implementation of 16S rRNA Gene Sequencing for Bacterial Identification in the Clinical Microbiology Laboratory.  American Society of Microbiology, Annual Meeting, Philadelphia, PA .  2009. 

Arbefeville S, Junkins A, Nasr M, Richter S, Bossler A.  Implementation of 16S rRNA Gene Sequencing for Bacterial Identification in the Clinical Microbiology Laboratory.  Department of Pathology Research Day.  2009. 

Gunsolly C, Bossler A.  Performance Evaluation of the COBAS® Ampliprep/COBAS® TaqMan® HCV Test.  University of Iowa, 2008, Department of Pathology Research Day and Research Week.  2009. 

Gunsolly C, Jordan J, Bossler A.  Performance of the cobas® 4800 HPV Test on SurePath Cytology Specimens.  Association for Molecular Pathology, Annual Meeting, Kissimmee, FL.  2009. 

Gunsolly C, Jordan J, Bossler A.  Performance of the cobas® 4800 HPV Test on SurePath Cytology Specimens.  University of Iowa, Department of Pathology Research Day.  2009. 

Harris G, Strand G, Gunsolly C, Lee J, Bossler A.  Pilot study to assess correlation of expression of PTPN13 in HPV-positive HNSCC with patient survival.  Otolaryngology Resident Research Day, University of Iowa.  2009. 

Spanos W, Hoover A, Harris G, Wu S, Strand G, Anderson M, Klingelhutz A, Hendriks W, Bossler A, Lee J.  The PDZ binding motif of human papillomavirus type 16 E6 induces PTPN13 loss, which allows anchorage-independent growth and synergizes with ras for invasive growth.  Journal of virology.  2008 March. 82(5):2493-500.
[PubMed]

Spanos W, Geiger J, Anderson M, Harris G, Bossler A, Smith R, Klingelhutz A, Lee J.  Deletion of the PDZ motif of HPV16 E6 preventing immortalization and anchorage-independent growth in human tonsil epithelial cells.  Head & neck.  2008 February. 30(2):139-47.
[PubMed]

Hoover A, Strand G, Nowicki P, Anderson M, Klingelhutz A, Bossler A, Lee J.  An Invasion Mechanism for HPV Related Cancers: HPV 16 E6 Degrades PTPN13 Allowing Enhanced MAP Kinase Signaling.  Molecular Biology of DNA Tumor Viruses, Madison, WI.  2008. 

VanDeerlin V, Bossler A.  Conventional and Real Time PCR.  Elsevier, New York.  2008. 

Strand G, Gunsolly C, Hoover A, Anderson M, Kuragp Z, Klingelhutz A, Lee j, Bossler A.  Loss of PTPN13 correlates with a subset of HPV positive oropharyngeal cancers.  Molecular Biology of DNA Tumor Viruses, Madison, WI.  2008. 

Strand G, Gunsolly C, Hoover A, Anderson M, Kurago Z, Klingelhutz A, Lee j, Bossler A.  Loss of PTPN13 correlates with a subset of HPV positive oropharyngeal cancers.  and University of Iowa, Department of Pathology Research Day.  2008. 

Barker S, McCormick R, Bedi j, Bossler A, Schneider E, Kalman L.  New Foci for the Genetic Testing Reference Material (GeT-RM) Program: Molecular Oncology and Infectious Disease.  Association for Molecular Pathology Annual Meeting.  2008. 

Barker S, McCormick R, Bedi j, Bossler A, Schneider E, Kalman L.  New Foci for the Genetic Testing Reference Material (GeT-RM) Program: Molecular Oncology and Infectious Disease.  University of Iowa, Department of Pathology Research Day.  2008. 

Gunsolly C, Bossler A.  Performance Evaluation of the COBAS® Ampliprep/COBAS® TaqMan® HCV Test.  Association for Molecular Pathology, Annual Meeting.  2008. 

Nowicki P, De Geest K, Goodheart M, Strand G, Bossler A, Lee J.  Protein tyrosine phosphatase (PTPN13) as a novel tumor suppressor in cervical carcinoma.  University of Iowa, College of Medicine; Research Week.  2008. 

Strand G, Gunsolly C, Hoover A, Anderson M, Lee J, Bossler A.  Role of PTPN13 in HPV related head and neck cancer.  University of Iowa, College of Medicine; Research Week.  2008. 

Bernardino L, Nagy P, Bossler A, Heusel J.  Simultaneous Detection of FLT3-ITD, FLT3-D835 and NPM1 mutations in AML.  Association for Molecular Pathology, Annual Meeting.  2008. 

Strand G, Gunsolly C, Anderson M, Klingelhutz A, Lee J, Bossler A.  Loss of PTPN13 expression correlates with a subset of HPV positive squamous cell carcinomas of the oropharynx.  University of Iowa, Department of Pathology Research Day.  2007. 

Wang H, Bossler A, Schaffer A, Tomczak E, DiPatri D, Frank D, Nowell P, Bagg A.  A novel t(3;8)(q27;q24.1) simultaneously involving both the BCL6 and MYC genes in a diffuse large B-cell lymphoma.  Cancer genetics and cytogenetics.  2007 January. 172(1):45-53.
[PubMed]

Bossler A, Richards J, George C, Godmilow L, Ganguly A.  Novel mutations in ENG and ACVRL1 identified in a series of 200 individuals undergoing clinical genetic testing for hereditary hemorrhagic telangiectasia (HHT): correlation of genotype with phenotype.  Human mutation.  2006 July. 27(7):667-75.
[PubMed]

Bossler A, Strubinger J, Gilmar C, Van Deerlin V.  Clinical laboratory testing for viral respiratory pathogens using real-time PCR is sensitive and rapid.  Association for Molecular Pathology Annual Meeting.  2006. 

Gunsolly C, Erwin M, Strand G, Richter S, Bossler A.  Comparison of rRNA gene sequencing with traditional biochemical testing for identification of unusual bacteria.  University of Iowa, Department of Pathology Research Day.  2006. 

Link B, Ballas Z, Weisdorf D, Wooldridge J, Bossler A, Shannon M, Rasmussen W, Krieg A, Weiner G.  Oligodeoxynucleotide CpG 7909 delivered as intravenous infusion demonstrates immunologic modulation in patients with previously treated non-Hodgkin lymphoma.  Journal of immunotherapy.  2006. 29(5):558-68.
[PubMed]

Bossler A, George C, Richards J, Godmilow L, Ganguly A.  Results from a clinical molecular screening program for hereditary hemorrhagic telangiectasia.  Association for Molecular Pathology Annual Meeting.  2005. 

Bossler A, Edler S, Forde S, Stence A, Cooper L, Murphy K, Kemp J, Kirby P, Heusel J.  Comparison of PCR-based methods for detection of chromosome 1p and 19q deletions in oligodendrogliomas.  Association for Molecular Pathology, Annual Meeting.  2004. 

Bossler A, Richter S, Chavez A, Vogelgesang S, Sutton D, Grooters A, Rinaldi M, de Hoog G, Pfaller M.  Exophiala oligosperma causing olecranon bursitis.  Journal of clinical microbiology.  2003 October. 41(10):4779-82.
[PubMed]

Soares M, Malchenko M, Martin J, Kucaba T, Scheetz T, Brown B, Smothers A, Huang J, Kuo S, Manzella L, Koroleva I, Bossler A, DeYoung B, Seftor E, Gitelis S, Hohl R, Buckwalter J, Hendrix M, Casavant T, Morcuende J.  Development of a comprehensive non-redundant collection of cDNAs/ESTs from human chondrosarcoma primary tumors and metastatic lung lesions.  American Association for Cancer Research, Annual Meeting.  2003. 

Bossler A, Richter S, Chavez A, Sutton D, Grooters A, Rinaldi M, deHoog G, Pfaller M.  Melanchlenus oligospermus and Prototheca wickerhamii causing olecranon bursitis.  American Society of Microbiology, Annual Meeting. Salt Lake City, UT.  2002. 

Bossler A, Kellokoski J, Lace M, Jäkel K, Anson J, Hubert W, Haugen T, Mitchell P, Turek L.  Cooperation of activator protein (AP)-2 alpha and transcriptional enhancer factor (TEF)-1 is necessary for the keratinocyte-dependent transcription and the establishment of infection by HPV types 16 and 31.  Experimental Biology, F.A.S.E.B Meeting, San Diego, CA.  2000. 

Bossler A.  Thesis: Keratinocyte-dependent regulation of the human papillomavirus oncogenes.  2000. 

Bossler A, Kellokoski J, Lace M, Jäkel K, Anson J, Hubert W, Haugen T, Mitchell P, Turek L.  Cooperation of activator protein (AP)-2 alpha and transcriptional enhancer factor (TEF)-1 is necessary for the keratinocyte-dependent transcription and the establishment of infection by HPV types 16 and 31.  Imperial Cancer Research Fund DNA Tumour Virus Meeting, Cambridge, UK.  1999. 

Bossler A, Kellokoski J, Lace M, Jäkel K, Anson J, Hubert W, Haugen T, Mitchell P, Turek L.  Cooperation of activator protein (AP)-2 and transcriptional enhancer factor (TEF)-1 is necessary for the keratinocyte-dependent transcription and the initial DNA replication of HPV types 16 and 31.  17th International Papillomavirus Conference, Charleston, SC.  1999. 

Bossler A, Kellokoski J, Lace M, Jäkel K, Anson J, Hubert W, Haugen T, Mitchell P, Turek L.  Cooperation of activator protein (AP)-2 alpha and transcriptional enhancer factor (TEF)-1 is necessary for the keratinocyte-dependent transcription of human papillomavirus (HPV)-16.  The 13th Annual National M.D./Ph.D. Student Conference, Aspen, CO.  1998. 

Bossler A, Kellokoski J, Lace M, Jäkel K, Anson J, Hubert W, Haugen T, Mitchell P, Turek L.  Cooperation of activator protein (AP)-2 alpha and transcriptional enhancer factor (TEF)-1 is necessary for the keratinocyte-dependent transcription of human papillomavirus (HPV)-16.  Molecular Biology of Small DNA Tumor Viruses, Madison, WI.  1998. 

Bossler A, Kellokoski J, Lace M, Jäkel  K, Anson J, Hubert W, Haugen T, Mitchell P, Turek L.  Cooperation of activator protein (AP)-2 and transcriptional enhancer factor (TEF)-1 is necessary and sufficient for the keratinocyte-dependent activation of a viral cis-element and necessary for E6-E7 promoter activity of HPV-16.  16th International Papillomavirus Conference, Siena, Italy.  1997. 

Bulinski J, Bossler A.  Purification and characterization of ensconsin, a novel microtubule stabilizing protein.  Journal of cell science.  1994 October. 107:2839-49.
[PubMed]

Date Last Modified: 07/31/2013 - 10:51:40