Ophthalmology And Visual Sciences

Budd A. Tucker, PhD

Portrait

Assistant Professor of Ophthalmology and Visual Sciences

Contact Information

Primary Office: 4111  MERF
Iowa City, IA 52242
Primary Office Phone: 319-353-4488

Email: budd-tucker@uiowa.edu
Web: Tucker Stem Cell Laboratory

Education

B.A.H., Biology, Sir Wilfred Grenfell College
PhD, Neuroscience, Memorial University of Newfoundland

Fellowship, Ophthalmology , Harvard Medical School

Education/Training Program Affiliations

Biosciences Graduate Program
Interdisciplinary Graduate Program in Molecular and Cellular Biology
Interdisciplinary Graduate Program in Neuroscience
Interdisciplinary Graduate Program in Translational Biomedicine

Research Summary

During my career I have had extensive experience and published several manuscripts/scientific abstracts relating to stem cell production, culture, isolation, differentiation and transplantation (via both cell injection and polymer scaffold mediated delivery). Two studies of particular importance to this proposal pertain to the use of skin-derived primary cells for the purpose of iPSC generation. The first, published in Plos One, was focused on the use of adult mouse skin derived iPSCs for the purpose of photoreceptor cell production and transplantation induced retinal regeneration. The second, published in PNAS, was focused on production of adult retinal disease specific iPSCs and subsequent human photoreceptors for the purpose of disease modeling and elucidation of disease mechanism. In both studies adult skin derived primary cells were essential for iPSC generation and subsequent experimentation. To date we have generated in excess of 300 molecularly confirmed disease specific human fibroblast and keratinocyte cell lines many of which have been reprogrammed to pluripotency and are now being used for disease modeling, drug screening, and cell transplantation studies.

Selected Publications

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Burnight E, Wiley L, Drack A, Braun T, Anfinson K, Kaalberg E, Halder J, Affatigato L, Mullins R, Stone E, Tucker B.  CEP290 gene transfer rescues Leber congenital amaurosis cellular phenotype.  Gene Ther.  2014. 21(7):662-72.
[PubMed]

Tucker B, Solivan-Timpe F, Roos B, Anfinson K, Robin A, Wiley L, Mullins R, Fingert J.  Duplication of TBK1 Stimulates Autophagy in iPSC-derived Retinal Cells from a Patient with Normal Tension Glaucoma.  Journal of Stem Cell Research and Therapy.  2014. 4(1):Article 1000161.

Mullins R, Khanna A, Schoo D, Tucker B, Sohn E, Drack A, Stone E.  Is age-related macular degeneration a microvascular disease?.  Adv Med Biol.  2014. 801:283-9.
[PubMed]

Sohn E, Flamme-Wiese M, Whitmore S, Wang K, Tucker B, Mullins R.  Loss of CD34 Expression in Aging Human Choriocapillaris Endothelial Cells.  PLoS One.  2014. 9(1):e86538.
[PubMed]

Baranov P, Tucker B, Young M.  Low-oxygen culture conditions extend the multipotent properties of human retinal progenitor cells.  Tissue engineering Part A.  2014. 20(9-10):1465-75.
[PubMed]

Worthington K, Wiley L, Bartlett A, Stone E, Mullins R, Salem A, Guymon C, Tucker B.  Mechanical properties of murine and porcine ocular tissues in compression.  Exp Eye Res.  2014. 121:194-9.
[PubMed]

Boye S, Huang W, Roman A, Sumaroka A, Boye S, Ryals R, Olivares M, Ruan Q, Tucker B, Stone E, Swaroop A, Cideciyan A, Hauswirth W, Jacobson S.  Natural History of Cone Disease in the Murine Model of Leber Congenital Amaurosis Due to CEP290 Mutation: Determining the Timing and Expectation of Therapy.  PLoS One.  2014. 9(3):e92928.
[PubMed]

Tucker B, Anfinson K, Mullins R, Stone E, Young M.  Use of a Synthetic Xeno-Free Culture Substrate for Induced Pluripotent Stem Cell Induction and Retinal Differentiation.  Stem Cells Transl Med.  2013 January. 2(1):16-24.
[PubMed]

Menke B, Salem A, Joshi V, Tucker B.  Enhanced Progenitor Cell Integration Following Transplantation of Progenitor Cell/MMP2-Polymer Constructs.  The Association for Research in Vision and Ophthalmology.  2012. 

Burnight E, Kaalberg K, Moses B, Mullins R, Stone E, Tucker B.  LCA Gene Therapy in Somatic-Cell-Derived Induced Pluripotent Stem Cells.  The Association for Research in Vision and Ophthalmology.  2012. 

Date Last Modified: 11/14/2014 - 14:33:09