Obstetrics and Gynecology

Pamela Geyer, PhD

Portrait

Professor of Biochemistry
Professor of Obstetrics and Gynecology

Contact Information

Primary Office: 3135E MERF
Iowa City, IA 52242
Primary Office Phone: 319-335-6953

Email: pamela-geyer@uiowa.edu
Web: Geyer Laboratory

Education

BS, McGill University, Montreal P.Q.
PhD, Ohio State University, Columbus, OH

Post Doctoral, Dept of Biology, Johns Hopkins University, Baltimore, MD
Post Doctoral, Dept of Biology, Johns Hopkins University, Baltimore, MD

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Biochemistry PhD
Interdisciplinary Graduate Program in Genetics
Interdisciplinary Graduate Program in Molecular and Cellular Biology
Interdisciplinary Graduate Program in Neuroscience
Interdisciplinary Graduate Program in Translational Biomedicine
Medical Scientist Training Program

Research Summary

The Geyer laboratory is interested in understanding mechanisms involved in genome organization and gene regulation. Two areas are currently being studied, insulators and nuclear lamina function. Insulators are a specialized class of DNA regulatory elements that have a conserved role in chromosome organization, dividing chromosomes into independent functional domains. These elements shelter genes from the surrounding positive and negative regulatory effects by blocking interactions between enhancers, silencers and promoters. Such properties have made insulators favorable candidates to improve strategies for gene therapy. While gene therapy is a promising technology that delivers therapeutic genes to a target tissue, successful application of this approach faces many challenges, including the cross regulation of endogenous genes by DNA regulatory elements in the vector. Insulators may prevent such undesirable effects. Studies in the Geyer lab include determination of the molecular mechanisms used by insulators to prevent interactions between enhancers, silencers and promoters and the identification of novel insulators. Combinations of insulators that employ different regulatory mechanisms may provide optimal protection of transgenes from the position effects of the surrounding chromatin and prevent sequences in the vector from mis-regulating endogenous genes at the site of genomic insertion. As such, insights into insulator mechanisms will allow advances in improving strategies for gene therapy and treatment of human disease. The nuclear lamina is an extensive protein network that lines the inner surface of the nuclear membrane. This network consists of intermediate filament proteins, called lamins, and associated inner nuclear membrane proteins, such as the LEM domain proteins. LEM domain genes make essential contributions to the function of the lamina, as mutations in these genes cause human diseases, known as laminopathies. For example, mutations in the genes that encode emerin and MAN1 cause Emery-Dreifuss Muscular Dystrophy and Buschke-Ollendorf Syndrome, respectively. Interestingly, these diseases are associated with tissue-specific pathologies, even though LEM domain genes are broadly expressed. To understand mechanisms underlying such human diseases, the Geyer lab is investigating the role of LEM domain proteins in lamina function, using Drosophila as a model organism. A molecular genetic approach is being taken to determine the contributions that individual LEM domain proteins make to nuclear lamina function.

Center, Program and Institute Affiliations

Institute for Clinical and Translational Science

All Publications

Barton L, Wilmington S, Martin M, Skopec H, Lovander K, Pinto B, Geyer P.  Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila, Genetics accepted..  Genetics Society of America;  2014. 

Soshnev A, Baxley R, Manak J, Tan K, Geyer P,  .  The insulator protein Suppressor of Hairy-wing is an essential transcriptional repressor in the Drosophila ovary.  Development.  2013 September. 140(17):3613-23.
[PubMed]

Barton L, Pinto B, Wallrath L, Geyer P.  The Drosophila nuclear lamina protein otefin is required for germline stem cell survival.  Developmental cell.  2013 June. 25(6):645-54.
[PubMed]

Hohl A, Thompson M, Soshnev A, Wu J, Morris J, Hsieh T, Wu C, Geyer P.  Restoration of topoisomerase 2 function by complementation of defective monomers in Drosophila.  Genetics.  2012 November. 192(3):843-56.
[PubMed]

Shoshnev A, He B, Baxley R, Jiang N, Hart C, Tan K, Geyer P.  Genome-wide studies of the multi-zinc finger Drosophila Suppressor of Hairy-wing protein in the ovary.  Nucleic Acids Research .  2012 July. 40(12).
[PubMed]

Soshnev A, Ishimoto H, McAllister B, Li X, Wehling M, Kitamoto T, Geyer P.  A conserved long noncoding RNA affects sleep behavior in Drosophila.  Genetics.  2011 October. 189(2):455-68.
[PubMed]

Baxley R, Soshnev A, Koryakov D, Zhimulev I, Geyer P.  The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis.  Developmental biology.  2011 August. 356(2):398-410.
[PubMed]

Geyer P, Vitalini M, Wallrath L.  Nuclear organization: taking a position on gene expression.  Current opinion in cell biology.  2011 June. 23(3):354-9.
[PubMed]

Dialynas G, Speese S, Budnik V, Geyer P, Wallrath L.  The role of Drosophila Lamin C in muscle function and gene expression.  Development (Cambridge, England).  2010 September. 137(18):3067-77.
[PubMed]

Schulze S, Curio-Penny B, Speese S, Dialynas G, Cryderman D, McDonough C, Nalbant D, Petersen M, Budnik V, Geyer P, Wallrath L.  A comparative study of Drosophila and human A-type lamins.  PloS one.  2009. 4(10):e7564.
[PubMed]

Wallrath L, Nagy P, Geyer P.  Epigenetics of development and human disease.  Mutation research.  2008 December. 647:1-2.
[PubMed]

Pinto B, Wilmington S, Hornick E, Wallrath L, Geyer P.  Tissue-specific defects are caused by loss of the Drosophila MAN1 LEM domain protein.  Genetics.  2008 September. 180(1):133-45.
[PubMed]

Kuhn-Parnell E, Helou C, Marion D, Gilmore B, Parnell T, Wold M, Geyer P.  Investigation of the properties of non-gypsy suppressor of hairy-wing-binding sites.  Genetics.  2008 July. 179(3):1263-73.
[PubMed]

Soshnev A, Li X, Wehling M, Geyer P.  Context differences reveal insulator and activator functions of a Su(Hw) binding region.  PLoS genetics.  2008. 4(8):e1000159.
[PubMed]

Baxley R, Geyer P.  The role of insulators in genome organization and gene expression.  The Encyclopedia of Life Sciences, John Wiley & Sons.  2008. 

Geyer P, Wallrath L.  Heterochromatin: not just for silencing anymore: report of the Eighth International Conference on Drosophila Heterochromatin.  Fly.  2007. 1(4):251-6.
[PubMed]

Golovnin A, Mazur A, Kopantseva M, Kurshakova M, Gulak P, Gilmore B, Whitfield W, Geyer P, Pirrotta V, Georgiev P.  Integrity of the Mod(mdg4)-67.2 BTB domain is critical to insulator function in Drosophila.  Molecular and cellular biology.  2007. 27:963-974.
[PubMed]

Parnell T, Kuhn E, Gilmore B, Helou C, Wold M, Geyer P.  Identification of genomic sites that bind the Drosophila suppressor of Hairy-wing insulator protein.  Molecular and cellular biology.  2006 August. 26(16):5983-93.
[PubMed]

Wallrath L, Geyer P.  TFIIIC boxes in the genome.  Cell.  2006 June. 125(5):829-31.
[PubMed]

Drapeau M, Cyran S, Viering M, Geyer P, Long A.  A cis-regulatory sequence within the yellow locus of Drosophila melanogaster required for normal male mating success.  Genetics.  2006 February. 172(2):1009-30.
[PubMed]

Schulze S, Curio-Penny B, Li Y, Imani R, Rydberg L, Geyer P, Wallrath L.  Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene.  Genetics.  2005 September. 171(1):185-96.
[PubMed]

Kuhn E, Hart C, Geyer P.  Studies of the role of the Drosophila scs and scs' insulators in defining boundaries of a chromosome puff.  Molecular and cellular biology.  2004 February. 24(4):1470-80.
[PubMed]

Wallrath L, Danzer J, Yazgan O, Geyer P.  Nuclear organization, chromatin structure and gene silencing.  in Encyclopedia of Biological Chemistry, Elsevier, Oxford.  2004. 3:105-108.

Parnell T, Viering M, Skjesol A, Helou C, Kuhn E, Geyer P.  An endogenous suppressor of hairy-wing insulator separates regulatory domains in Drosophila.  Proceedings of the National Academy of Sciences of the United States of America.  2003 November. 100(23):13436-41.
[PubMed]

Kuhn E, Geyer P.  Genomic insulators: connecting properties to mechanism.  Current opinion in cell biology.  2003 June. 15(3):259-65.
[PubMed]

Kuhn E, Viering M, Rhodes K, Geyer P.  A test of insulator interactions in Drosophila.  The EMBO journal.  2003 May. 22(10):2463-71.
[PubMed]

Parnell T, Grade S, Geyer P, Wallrath L.  Position-effect variegation in human genetic disease.  Encyclopedia of the Human Genome. John Wiley & Sons, Ltd.  2003. 4:642-46.

Geyer P, Clark I.  Protecting against promiscuity: the regulatory role of insulators.  Cellular and molecular life sciences : CMLS.  2002 December. 59(12):2112-27.
[PubMed]

Chen J, Huisinga K, Viering M, Ou S, Wu C, Geyer P.  Enhancer action in trans is permitted throughout the Drosophila genome.  Proceedings of the National Academy of Sciences of the United States of America.  2002 March. 99(6):3723-8.
[PubMed]

Roseman R, Morgan K, Mallin D, Roberson R, Parnell T, Bornemann D, Simon J, Geyer P.  Long-range repression by multiple polycomb group (PcG) proteins targeted by fusion to a defined DNA-binding domain in Drosophila.  Genetics.  2001 May. 158(1):291-307.
[PubMed]

Kuhn E, Geyer P.  Boundary Elements.  Encyclopedia of Molecular Medicine, John Wiley & Sons.  2001. 

Geyer P, Parnell T.  Chromosomes: Higher order organization.  Encyclopedia of Life Sciences. London: Naturing Publishing Group.  2001. 

Parnell T, Geyer P.  Differences in insulator properties revealed by enhancer blocking assays on episomes.  The EMBO journal.  2000 November. 19(21):5864-74.
[PubMed]

Scott K, Taubman A, Geyer P.  Enhancer blocking by the Drosophila gypsy insulator depends upon insulator anatomy and enhancer strength.  Genetics.  1999 October. 153(2):787-98.
[PubMed]

Morris J, Chen J, Filandrinos S, Dunn R, Fisk R, Geyer P, Wu C.  An analysis of transvection at the yellow locus of Drosophila melanogaster.  Genetics.  1999 February. 151(2):633-51.
[PubMed]

Morris J, Geyer P, Wu C.  Core promoter elements can regulate transcription on a separate chromosome in trans.  Genes & development.  1999 February. 13(3):253-8.
[PubMed]

Morris J, Chen J, Geyer P, Wu C.  Two modes of transvection: enhancer action in trans and bypass of a chromatin insulator in cis.  Proceedings of the National Academy of Sciences of the United States of America.  1998 September. 95(18):10740-5.
[PubMed]

Mallin D, Myung J, Patton J, Geyer P.  Polycomb group repression is blocked by the Drosophila suppressor of Hairy-wing [su(Hw)] insulator.  Genetics.  1998 January. 148(1):331-9.
[PubMed]

Geyer P.  The role of insulator elements in defining domains of gene expression.  Current opinion in genetics & development.  1997 April. 7(2):242-8.
[PubMed]

Scott K, Geyer P.  Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes.  The EMBO journal.  1995 December. 14(24):6258-67.
[PubMed]

Roseman R, Swan J, Geyer P.  A Drosophila insulator protein facilitates dosage compensation of the X chromosome min-white gene located at autosomal insertion sites.  Development (Cambridge, England).  1995 November. 121(11):3573-82.
[PubMed]

Roseman R, Johnson E, Rodesch C, Bjerke M, Nagoshi R, Geyer P.  A P element containing suppressor of hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster.  Genetics.  1995 November. 141(3):1061-74.
[PubMed]

Rodesch C, Geyer P, Patton J, Bae E, Nagoshi R.  Developmental analysis of the ovarian tumor gene during Drosophila oogenesis.  Genetics.  1995 September. 141(1):191-202.
[PubMed]

Nagoshi R, Patton J, Bae E, Geyer P.  The somatic sex determines the requirement for ovarian tumor gene activity in the proliferation of the Drosophila germline.  Development (Cambridge, England).  1995 February. 121(2):579-87.
[PubMed]

Bae E, Cook K, Geyer P, Nagoshi R.  Molecular characterization of ovarian tumors in Drosophila.  Mechanisms of development.  1994 August. 47(2):151-64.
[PubMed]

Geyer P, Patton J, Rodesch C, Nagoshi R.  Genetic and molecular characterization of P element-induced mutations reveals that the Drosophila ovarian tumor gene has maternal activity and a variable null phenotype.  Genetics.  1993 February. 133(2):265-78.
[PubMed]

Roseman R, Pirrotta V, Geyer P.  The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects.  The EMBO journal.  1993 February. 12(2):435-42.
[PubMed]

Patton J, Gomes X, Geyer P.  Position-independent germline transformation in Drosophila using a cuticle pigmentation gene as a selectable marker.  Nucleic acids research.  1992 November. 20(21):5859-60.
[PubMed]

Geyer P, Corces V.  DNA position-specific repression of transcription by a Drosophila zinc finger protein.  Genes & development.  1992 October. 6(10):1865-73.
[PubMed]

Geyer P, Chien A, Corces V, Green M.  Mutations in the su(s) gene affect RNA processing in Drosophila melanogaster.  Proceedings of the National Academy of Sciences of the United States of America.  1991 August. 88(16):7116-20.
[PubMed]

Corces V, Geyer P.  Interactions of retrotransposons with the host genome: the case of the gypsy element of Drosophila.  Trends in genetics : TIG.  1991 March. 7(3):86-90.
[PubMed]

Geyer P, Green M, Corces V.  Tissue-specific transcriptional enhancers may act in trans on the gene located in the homologous chromosome: the molecular basis of transvection in Drosophila.  The EMBO journal.  1990 July. 9(7):2247-56.
[PubMed]

Harrison D, Geyer P, Spana C, Corces V.  The gypsy retrotransposon of Drosophila melanogaster: mechanisms of mutagenesis and interaction with the suppressor of Hairy-wing locus.  Developmental genetics.  1989. 10(3):239-48.
[PubMed]

Geyer P, Green M, Corces V.  Mutant gene phenotypes mediated by a Drosophila melanogaster retrotransposon require sequences homologous to mammalian enhancers.  Proceedings of the National Academy of Sciences of the United States of America.  1988 November. 85(22):8593-7.
[PubMed]

Geyer P, Richardson K, Corces V, Green M.  Genetic instability in Drosophila melanogaster: P-element mutagenesis by gene conversion.  Proceedings of the National Academy of Sciences of the United States of America.  1988 September. 85(17):6455-9.
[PubMed]

Geyer P, Green M, Corces V.  Reversion of a gypsy-induced mutation at the yellow (y) locus of Drosophila melanogaster is associated with the insertion of a newly defined transposable element.  Proceedings of the National Academy of Sciences of the United States of America.  1988 June. 85(11):3938-42.
[PubMed]

Geyer P, Corces V.  Separate regulatory elements are responsible for the complex pattern of tissue-specific and developmental transcription of the yellow locus in Drosophila melanogaster.  Genes & development.  1987 November. 1(9):996-1004.
[PubMed]

Geyer P, Green M, Corces V.  Molecular basis of transposable element-induced mutations in Drosophila melanogaster.  Eukaryotic Transposable Elements as Mutagenic Agents: CSHLP, Cold Spring Harbor, N.Y.  1987. 

Geyer P, Fyrberg E.  5'-flanking sequence required for regulated expression of a muscle-specific Drosophila melanogaster actin gene.  Molecular and cellular biology.  1986 October. 6(10):3388-96.
[PubMed]

Geyer P, Spana C, Corces V.  On the molecular mechanism of gypsy-induced mutations at the yellow locus of Drosophila melanogaster.  The EMBO journal.  1986 October. 5(10):2657-62.
[PubMed]

Jenh C, Geyer P, Johnson L.  Control of thymidylate synthase mRNA content and gene transcription in an overproducing mouse cell line.  Molecular and cellular biology.  1985 October. 5(10):2527-32.
[PubMed]

Jenh C, Geyer P, Baskin F, Johnson L.  Thymidylate synthase gene amplification in fluorodeoxyuridine-resistant mouse cell lines.  Molecular pharmacology.  1985 July. 28(1):80-5.
[PubMed]

Geyer P, Johnson L.  Molecular cloning of DNA sequences complementary to mouse thymidylate synthase messenger RNA.  The Journal of biological chemistry.  1984 June. 259(11):7206-11.
[PubMed]

Geyer P, Meyuhas O, Perry R, Johnson L.  Regulation of ribosomal protein mRNA content and translation in growth-stimulated mouse fibroblasts.  Molecular and cellular biology.  1982 June. 2(6):685-93.
[PubMed]

Date Last Modified: 06/07/2014 - 21:56:23