Microbiology

George V. Stauffer, PhD

Portrait

Contact Information

Office: 3-352 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
Office Phone: 319-335-7791

Lab: 3-315 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
Phone: 319-335-8866

Email: george-stauffer@uiowa.edu

Education

BS, Pennsylvania State University
MS, Pennsylvania State University
PhD, Pennsylvania State University

Post Doctoral, Stanford University

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Microbiology Graduate Program

Research Summary

Our long-term objective is to understand the basic principles of gene regulation. In Escherichia coli, the gcvB gene encodes a small non-translated RNA that in turn regulates other pathways (e.g., oppA, dppA, cycA and sstT, encoding the oligopeptide and dipeptide periplasmic binding proteins, glycine transport protein and serine transport protein, respectively). The gcvB gene is activated by the GcvA protein in response to glycine and repressed by the GcvA + GcvR proteins in the absence of glycine. Because expression of gcvB follows a pattern of regulation qualitatively similar to the gcvTHP operon, encoding the glycine cleavage enzyme (GCV) complex for generating one-carbon units for cellular methylation reactions, we hypothesize GcvB plays a role in an integrated cellular response to regulate other genes in conjunction with the GCV enzyme system. Using genetics, physiology and biochemistry, we will address two major questions. First, we will determine the secondary structure of GcvB and define regions important for interacting with target mRNAs and regulatory proteins. Second, we will determine the regulatory network controlled by GcvB to gain insight into its role in cell physiology and the molecular mechanisms of control by GcvB.

Center, Program and Institute Affiliations

Center for Biocatalysis and Bioprocessing
Specialized Center for Research in Osteoarthritis

All Publications

Stauffer L, Stauffer G.  Multiple roles for the sRNA GcvB in the Regulation of Slp Levels in Escherichia coli.  ISRN Bacteriology.  2013. 2013:918106.

Stauffer L, Stauffer G.  The Escherichia coli GcvB sRNA uses genetic redundancy to control cycA expression.  ISRN Microbiol.  2012 May 28. 2012:636273.
[Link]

Stauffer L, Stauffer G.  Antagonistic roles for GcvA and GcvB in hdeAB expression in Escherichia coli.  ISRN Microbiol.  2012 May 16. 2012:697308.
[Link]

Pulvermacher S, Stauffer L, Stauffer G.  Role of the Escherichia coli Hfq protein in GcvB regulation of oppA and dppA mRNAs.  Microbiology.  2009 January. 155(Pt 1):115-23.
[Link]

Pulvermacher S, Stauffer L, Stauffer G.  Role of the sRNA GcvB in regulation of cycA in Escherichia coli.  Microbiology.  2009 January. 155(Pt 1):106-14.
[Link]

Pulvermacher S, Stauffer L, Stauffer G.  The small RNA GcvB regulates sstT mRNA expression in Escherichia coli.  J Bacteriol.  2009 January. 191(1):238-48.
[Link]

Pulvermacher S, Stauffer L, Stauffer G.  The role of the small regulatory RNA GcvB in GcvB/mRNA posttranscriptional regulation of oppA and dppA in Escherichia coli.  FEMS Microbiol Lett.  2008 April. 281(1):42-50.
[Link]

Pulvermacher S, Stauffer G.  The sRNA GcvB Negatively Regulated sstT mRNA in Escherichia coli.  Abstr 108th Gen Meet Amer Soc Microbiol.  2008. 

Hall M, Stauffer G.  The involvement of RNase E and RNase III in GcvB mediated regulation of dppA and oppA in Escherichia coli.  Abstr 107th Gen Meet Amer Soc Microbiol.  2007. 

Pulvermacher S, Stauffer G.  The role of RNA/RNA pairing for the gcvB-encoded small RNAs in regulating oppA and dppA in Escherichia coli.  Abstr 107th Gen Meet Amer Soc Microbiol.  2007. 

McArthur S, Pulvermacher S, Stauffer G.  The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules.  BMC Microbiol.  2006 June. 6:52.
[Link]

Pulvermacher S, Stauffer G.  The GcvB sRNAs are Dependent upon Hfq for Regulation.  Abstr 106th Gen Meet Amer Soc Microbiol.  2006. 

Stauffer L, Stauffer G.  GcvA interacts with both the alpha and sigma subunits of RNA polymerase to activate the Escherichia coli gcvB gene and the gcvTHP operon.  FEMS Microbiol Lett.  2005 January 15. 242(2):333-8.
[Link]

Stauffer G.  EcoSal module. 3.6.1.2 Serine, glycine and one-carbon metabolism.  American Society for Microbiology, Washington, DC.  2004. 

McArthur S, Stauffer K, Stauffer G.  The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules.  Abstr 104th Gen Meet Amer Soc Microbiol.  2004. 

Heil G, Stauffer L, Stauffer G.  Glycine binds the transcriptional accessory protein GcvR to disrupt a GcvA/GcvR interaction and allow GcvA-mediated activation of the Escherichia coli gcvTHP operon.  Microbiology.  2002 July. 148(Pt 7):2203-14.
[Link]

Ghrist A, Heil G, Stauffer G.  GcvR interacts with GcvA to inhibit activation of the Escherichia coli glycine cleavage operon.  Microbiology.  2001 August. 147(Pt 8):2215-21.
[Link]

Wonderling L, Urbanowski M, Stauffer G.  GcvA binding site 1 in the gcvTHP promoter of Escherichia coli is required for GcvA-mediated repression but not for GcvA-mediated activation.  Microbiology.  2000 November. 146 ( Pt 11):2909-18.
[Link]

Fritsch P, Urbanowski M, Stauffer G.  Role of the RNA polymerase alpha subunits in MetR-dependent activation of metE and metH: important residues in the C-terminal domain and orientation requirements within RNA polymerase.  J Bacteriol.  2000 October. 182(19):5539-50.
[Link]

Urbanowski M, Stauffer L, Stauffer G.  The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli.  Mol Microbiol.  2000 August. 37(4):856-68.
[Link]

Jourdan A, Stauffer G.  GcvA-mediated activation of gcvT-lacZ expression involves the carboxy-terminal domain of the alpha subunit of RNA polymerase.  FEMS Microbiol Lett.  1999 December 15. 181(2):307-12.
[Link]

Jourdan A, Stauffer G.  Genetic analysis of the GcvA binding site in the gcvA control region.  Microbiology.  1999 August. 145(Pt 8):2153-62.
[Link]

Stauffer L, Stauffer G.  Role for the leucine-responsive regulatory protein (Lrp) as a structural protein in regulating the Escherichia coli gcvTHP operon.  Microbiology.  1999 March. 145(Pt 3):569-76.
[Link]

Wonderling L, Stauffer G.  The cyclic AMP receptor protein is dependent on GcvA for regulation of the gcv operon.  J Bacteriol.  1999 March. 181(6):1912-9.
[Link]

Stauffer L, Stauffer G.  Roles for GcvA-binding sites 3 and 2 and the Lrp-binding region in gcvT::lacZ expression in Escherichia coli.  Microbiology.  1998 October. 144(Pt 10):2865-72.
[Link]

Jourdan A, Stauffer G.  Mutational analysis of the transcriptional regulator GcvA: amino acids important for activation, repression, and DNA binding.  J Bacteriol.  1998 September. 180(18):4865-71.
[Link]

Stauffer L, Stauffer G.  Spacing and orientation requirements of GcvA-binding sites 3 and 2 and the Lrp-binding region for gcvT::lacZ expression in Escherichia coli.  Microbiology.  1998 May. 144(Pt 5):1417-22.
[Link]

Ghrist A, Stauffer G.  Promoter characterization and constitutive expression of the Escherichia coli gcvR gene.  J Bacteriol.  1998 April. 180(7):1803-7.
[Link]

Fritsch P, Urbanowski M, Stauffer G.  MetR-dependent activation of metE and metH requires the carboxy-terminal domain of the RNA polymerase alpha subunit.  Cold Spring Harbor Meeting on Molecular Genetics of Bacteria & Phages.  1998. 

Jourdan A, Stauffer G.  A positive control mutant of the transcriptional regulator GcvA.  Abstr Meet Amer Soc Microbiol.  1997. 

Wonderling L, Stauffer G.  The effect of Crp on gcv operon expression requires the GcvA protein.  Abstr Meet Amer Soc Microbiol.  1997. 

Carlson P, Urbanowski M, Stauffer G.  The effect of mutations in the Excherichia coli RNA polymerase alpha subunit on MetR activation of metE and metH.  Abstr Meet Amer Soc Microbiol.  1997. 

Jafri S, Urbanowski M, Stauffer G.  The glutamic acid residue at amino acid 261 of the alpha subunit is a determinant of the intrinsic efficiency of RNA polymerase at the metE core promoter in Escherichia coli.  J Bacteriol.  1996 December. 178(23):6810-6.
[Link]

Lorenz E, Stauffer G.  MetR-mediated repression of the glyA gene in Escherichia coli.  FEMS Microbiol Lett.  1996 November 1. 144(2-3):229-33.
[Link]

Lorenz E, Stauffer G.  RNA polymerase, PurR and MetR interactions at the glyA promoter of Escherichia coli.  Microbiology.  1996 July. 142 ( Pt 7):1819-24.
[Link]

De Groote M, Testerman T, Xu Y, Stauffer G, Fang F.  Homocysteine antagonism of nitric oxide-related cytostasis in Salmonella typhimurium.  Science.  1996 April 19. 272(5260):414-7.
[Link]

Lorenz E, Stauffer G.  Cooperative MetR binding in the Escherichia coli glyA control region.  FEMS Microbiol Lett.  1996 April 1. 137(2-3):147-52.
[Link]

Lorenz E, Plamann M, Stauffer G.  Escherichia coli cis- and trans-acting mutations that increase glyA gene expression.  Mol Gen Genet.  1996 January 15. 250(1):81-8.
[Link]

Jourdan A, Stauffer G.  An autoregulatory mutation in gcvA that relieves purine-mediated repression of the gcv operon.  Abstr 96th Gen Meet Amer Soc Microbiol.  1996. 

Stauffer G.  Biosynthesis of serine glycine and one-carbon units.  American Society for Microbiology, Washington, DC.  1996. 2:506-13.

Ghrist A, Stauffer L, Stauffer G.  Characterization of gcvR, a negative regulator of gcv expression.  Abstr 96th Gen Meet Amer Soc Microbiol.  1996. 

Carlson P, Urbanowski M, Stauffer G.  Mutations in RNA polymerase alpha subunit that affect MetR activation of metE.  Abstr 96th Gen Meet Amer Soc Microbiol.  1996. 

Wonderling L, Stauffer G.  The cAMP receptor protein is involved in activation of the gcv operon.  Abstr 96th Gen Meet Amer Soc Microbiol.  1996. 

Ghrist A, Stauffer G.  Characterization of the Escherichia coli gcvR gene encoding a negative regulator of gcv expression.  J Bacteriol.  1995 September. 177(17):4980-4.
[Link]

Wilson R, Urbanowski M, Stauffer G.  DNA binding sites of the LysR-type regulator GcvA in the gcv and gcvA control regions of Escherichia coli.  J Bacteriol.  1995 September. 177(17):4940-6.
[Link]

Lorenz E, Stauffer G.  Characterization of the MetR binding sites for the glyA gene of Escherichia coli.  J Bacteriol.  1995 July. 177(14):4113-20.
[Link]

Wu W, Urbanowski M, Stauffer G.  Characterization of a second MetR-binding site in the metE metR regulatory region of Salmonella typhimurium.  J Bacteriol.  1995 April. 177(7):1834-9.
[Link]

Jafri S, Urbanowski M, Stauffer G.  A mutation in the rpoA gene encoding the alpha subunit of RNA polymerase that affects metE-metR transcription in Escherichia coli.  J Bacteriol.  1995 February. 177(3):524-9.
[Link]

Lorenz E, Stauffer G.  MetR binding to the glyA control region results in DNA bending.  Abstr Ann Meet Amer Soc Microbiol.  1995. 

Ghrist A, Stauffer G.  The Escherichia coli glycine transport system and its role in the regulation of the glycine cleavage enzyme system.  Microbiology.  1995 January. 141(Pt 1):133-40.
[Link]

Stauffer L, Stauffer G.  Characterization of the gcv control region from Escherichia coli.  J Bacteriol.  1994 October. 176(20):6159-64.
[Link]

Stauffer L, Fogarty S, Stauffer G.  Characterization of the Escherichia coli gcv operon.  Gene.  1994 May 3. 142(1):17-22.
[Link]

Wilson R, Stauffer G.  DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system.  J Bacteriol.  1994 May. 176(10):2862-8.
[Link]

Urbanowski M, Stauffer G.  Genetic analysis of the functional domains of the Salmonella typhimurium MetR activator.  Abstr Ann Meet Amer Soc Microbiol.  1994. 

Jafri S, Stauffer G.  Isolation and characterization of a mutation in the alpha subunit of RNA polymerase that affects MetE transcription in E. coli.  Abstr Ann Meet Amer Soc Microbiol.  1994. 

Ghrist A, Stauffer G.  Isolation of an Escherichia coli mutant with altered purine-mediated regulation of the glycine cleavage enzyme system.  Abstr Ann Meet Amer Soc Microbiol.  1994. 

Lorenz E, Stauffer G.  MetR binding to the glyA control region results in DNA binding.  Abstr Ann Meet Amer Soc Microbiol.  1994. 

Cowan J, Urbanowski M, Talmi M, Stauffer G.  Regulation of the Salmonella typhimurium metF gene by the MetR protein.  J Bacteriol.  1993 September. 175(18):5862-6.
[Link]

Wilson R, Stauffer L, Stauffer G.  Roles of the GcvA and PurR proteins in negative regulation of the Escherichia coli glycine cleavage enzyme system.  J Bacteriol.  1993 August. 175(16):5129-34.
[Link]

Wu W, Urbanowski M, Stauffer G.  MetJ-mediated regulation of the Salmonella typhimurium metE and metR genes occurs through a common operator region.  FEMS Microbiol Lett.  1993 April 1. 108(2):145-50.
[Link]

Wilson R, Steiert P, Stauffer G.  Positive regulation of the Escherichia coli glycine cleavage enzyme system.  J Bacteriol.  1993 February. 175(3):902-4.
[Link]

Urbanowski M, Stauffer G.  An indirect role for homocysteine in activation of the S. typhimurium metH gene.  Abstr Ann Meet Amer Soc Microbiol.  1993. 

Cowan J, Stauffer G.  Analysis of the MetR binding site for the S. typhimurium metF gene.  Abstr Ann Meet Amer Soc Microbiol.  1993. 

Stauffer G, Stauffer L.  Deletion analysis of the gcv control region from Escherichia coli.  Abstr Ann Meet Amer Soc Microbiol.  1993. 

Stauffer L, Ghrist A, Stauffer G.  The Escherichia coli gcvT gene encoding the T-protein of the glycine cleavage enzyme system.  DNA Seq.  1993. 3(6):339-46.
[Link]

Steiert J, Kubu C, Stauffer G.  The PurR binding site in the glyA promoter region of Escherichia coli.  FEMS Microbiol Lett.  1992 December 1. 78(2-3):299-304.
[Link]

Neuwald A, Berg D, Stauffer G.  Mutational analysis of the Escherichia coli serB promoter region reveals transcriptional linkage to a downstream gene.  Gene.  1992 October 12. 120(1):1-9.
[Link]

Wu W, Urbanowski M, Stauffer G.  Role of the MetR regulatory system in vitamin B12-mediated repression of the Salmonella typhimurium metE gene.  J Bacteriol.  1992 July. 174(14):4833-7.
[Link]

Lorenz E, Steiert P, Stauffer G.  Identification of a trans-acting factor involved in glyA gene control.  Abstr Ann Meet Amer Soc Microbiol.  1992. 

Wilson R, Urbanowski M, Stauffer G.  Negative regulation of the Escherichia coli glycine cleavage enzyme system by the purR gene product.  Abstr Ann Meet Amer Soc Microbiol.  1992. 

Cowan J, Stauffer G.  Regulation of metF gene expression by MetR protein in Salmonella typhimurium.  Abstr Ann Meet Amer Soc Microbiol.  1992. 

Mares R, Urbanowski M, Stauffer G.  Regulation of the Salmonella typhimurium metA gene by the metR protein and homocysteine.  J Bacteriol.  1992 January. 174(2):390-7.
[Link]

Bae Y, Stauffer G.  Mutations that affect activity of the Rhizobium meliloti trpE(G) promoter in Rhizobium meliloti and Escherichia coli.  J Bacteriol.  1991 September. 173(18):5831-6.
[Link]

Bae Y, Stauffer G.  Genetic analysis of the attenuator of the Rhizobium meliloti trpE(G) gene.  J Bacteriol.  1991 June. 173(11):3382-8.
[Link]

Byerly K, Urbanowski M, Stauffer G.  The metR binding site in the Salmonella typhimurium metH gene: DNA sequence constraints on activation.  J Bacteriol.  1991 June. 173(11):3547-53.
[Link]

Stauffer L, Steiert P, Steiert J, Stauffer G.  An Escherichia coli protein with homology to the H-protein of the glycine cleavage enzyme complex from pea and chicken liver.  DNA Seq.  1991. 2(1):13-7.
[Link]

Stauffer G, Wilson R, Steiert P.  Positive regulation of the Escherichia coli glycine cleavage enzyme system.  Abstr Ann Meet Amer Soc Microbiol.  1991. 

Loewen P, Stauffer G.  Nucleotide sequence of katG of Salmonella typhimurium LT2 and characterization of its product, hydroperoxidase I.  Mol Gen Genet.  1990 October. 224(1):147-51.
[Link]

Steiert P, Stauffer L, Stauffer G.  The lpd gene product functions as the L protein in the Escherichia coli glycine cleavage enzyme system.  J Bacteriol.  1990 October. 172(10):6142-4.
[Link]

Steiert J, Rolfes R, Zalkin H, Stauffer G.  Regulation of the Escherichia coli glyA gene by the purR gene product.  J Bacteriol.  1990 July. 172(7):3799-803.
[Link]

Byerly K, Urbanowski M, Stauffer G.  Escherichia coli metR mutants that produce a MetR activator protein with an altered homocysteine response.  J Bacteriol.  1990 June. 172(6):2839-43.
[Link]

Neuwald A, Stauffer G.  IS30 activation of an smp'-lacZ gene fusion in Escherichia coli.  FEMS Microbiol Lett.  1990 March 1. 56(1-2):13-7.
[Link]

Eberly L, Stauffer G.  Amino acid changes in the trpB gene that result in a "repairable" phenotype.  Genetics.  1990. 21:1.183 p 67.

Byerly K, Urbanowski M, Stauffer G.  Analysis of the MetR binding site in the metH control region of Salmonella typhimurium.  Abstr Ann Meet Amer Soc Microbiol.  1990. 

Bae Y, Stauffer G.  Mutational studies on the attenuator of the Rhizobium meliloti trpE(G) gene.  Genetics.  1990. 21:1.174 p 65.

Steiert J, Urbanowski M, Stauffer L, Plamann M, Stauffer G.  Nucleotide sequence of the Salmonella typhimurium glyA gene.  DNA Seq.  1990. 1(2):107-13.
[Link]

Steiert J, Stauffer G.  Regulation of the glyA gene in E. coli by the MetR and PurR proteins.  Genetics.  1990. 21:1.208 p 73.

Mares R, Urbanowski M, Stauffer G.  Regulation of the Salmonella typhimurium metA gene by the metR protein and homocysteine.  Abstr Ann Meet Amer Soc Microbiol.  1990. 

Urbanowski M, Stauffer G.  Temperature-dependent selective repression of the metE and metF genes by the MetJ repressor in E. coli.  Genetics.  1990. 21:1.214 p 75.

Plamann M, Stauffer G.  Escherichia coli glyA mRNA decay: the role of 3' secondary structure and the effects of the pnp and rnb mutations.  Mol Gen Genet.  1990 January. 220(2):301-6.
[Link]

Stauffer G, Stauffer L, Plamann M.  The Salmonella typhimurium glycine cleavage enzyme system.  Mol Gen Genet.  1989 December. 220(1):154-6.
[Link]

Neuwald A, Stauffer G.  An Escherichia coli membrane protein with a unique signal sequence.  Gene.  1989 October 30. 82(2):219-28.
[Link]

Urbanowski M, Stauffer G.  Genetic and biochemical analysis of the MetR activator-binding site in the metE metR control region of Salmonella typhimurium.  J Bacteriol.  1989 October. 171(10):5620-9.
[Link]

Plamann M, Stauffer G.  Regulation of the Escherichia coli glyA gene by the metR gene product and homocysteine.  J Bacteriol.  1989 September. 171(9):4958-62.
[Link]

Park Y, Stauffer G.  Salmonella typhimurium metC operator-constitutive mutations.  FEMS Microbiol Lett.  1989 July 15. 51(1):137-41.
[Link]

Urbanowski M, Stauffer G.  Role of homocysteine in metR-mediated activation of the metE and metH genes in Salmonella typhimurium and Escherichia coli.  J Bacteriol.  1989 June. 171(6):3277-81.
[Link]

Park Y, Stauffer G.  DNA sequence of the metC gene and its flanking regions from Salmonella typhimurium LT2 and homology with the corresponding sequence of Escherichia coli.  Mol Gen Genet.  1989 March. 216(1):164-9.
[Link]

Steiert P, Stauffer L, Stauffer G.  Characterization of a new class of glycine cleavage enzyme system mutants in Escherichia coli.  Abstr Ann Meet Amer Soc Microbiol.  1989. 

Brot N, Maxon M, Cai X, Redfield B, Fujita K, Stauffer G, Old I, Glass R, Weissbach H.  Effect of MetR protein on the in vitro expression of the metC, metH and metR genes.  ASBMB Meeting.  1989. 

Byerly K, Stauffer G.  Escherichia coli mutants that produce a MetR activator protein with an altered homocysteine response.  Abstr Ann Meet Amer Soc Microbiol.  1989. 

Steiert J, Urbanowski M, Stauffer G.  Regulation of the Escherichia coli glyA gene and a divergently transcribed gene by the metR gene product.  Abstr Ann Meet Amer Soc Microbiol.  1989. 

Maxon M, Redfield B, Cai X, Shoeman R, Fujita K, Fisher W, Stauffer G, Weissbach H, Brot N.  Regulation of methionine synthesis in Escherichia coli: effect of the MetR protein on the expression of the metE and metR genes.  Proc Natl Acad Sci U S A.  1989 January. 86(1):85-9.
[Link]

Plamann L, Urbanowski M, Stauffer G.  Salmonella typhimurium metE operator-constitutive mutations.  Gene.  1988 December 15. 73(1):201-8.
[Link]

Urbanowski M, Stauffer G.  The control region of the metH gene of Salmonella typhimurium LT2: an atypical met promoter.  Gene.  1988 December 15. 73(1):193-200.
[Link]

Stauffer G, Stauffer L.  Salmonella typhimurium LT2 metF operator mutations.  Mol Gen Genet.  1988 September. 214(1):32-6.
[Link]

Stauffer G, Stauffer L.  Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF gene and its homology with the corresponding sequence of Escherichia coli.  Mol Gen Genet.  1988 May. 212(2):246-51.
[Link]

Park Y, Stauffer G.  Mutations that affect expression of the metC gene of Salmonella typhimurium.  Abstr Ann Meet Amer Soc Microbiol.  1988. 

Urbanowski M, Stauffer G.  Regulation of the metR gene of Salmonella typhimurium.  J Bacteriol.  1987 December. 169(12):5841-4.
[Link]

Plamann L, Stauffer G.  Nucleotide sequence of the Salmonella typhimurium metR gene and the metR-metE control region.  J Bacteriol.  1987 September. 169(9):3932-7.
[Link]

Urbanowski M, Stauffer L, Plamann L, Stauffer G.  A new methionine locus, metR, that encodes a trans-acting protein required for activation of metE and metH in Escherichia coli and Salmonella typhimurium.  J Bacteriol.  1987 April. 169(4):1391-7.
[Link]

Stauffer G.  Biosynthesis of serine and glycine.  American Society for Microbiology, Washington, DC.  1987. 1:412-8.

Park Y, Stauffer G.  Characterization of the metC gene and its flanking regions from Salmonella typhimurium.  Abstr Ann Meet Amer Soc Microbiol.  1987. 

Park Y, Stauffer G.  Cloning and characterization of the metC gene from Salmonella typhimurium LT2.  Gene.  1987. 60(2-3):291-7.
[Link]

Plamann L, Urbanowski M, Stauffer G.  Identification of the Salmonella typhimurium metR gene product and biochemical characterization of the metR-metE control region.  J Cell Biochem.  1987. 35(Supplement 11C):57, Abstract L132.

Urbanowski M, Plamann L, Stauffer G.  Mutations affecting the regulation of the metB gene of Salmonella typhimurium LT2.  J Bacteriol.  1987 January. 169(1):126-30.
[Link]

Urbanowski M, Stauffer G.  Autoregulation by tandem promoters of the Salmonella typhimurium LT2 metJ gene.  J Bacteriol.  1986 March. 165(3):740-5.
[Link]

Stauffer L, Plamann M, Stauffer G.  Cloning and characterization of the glycine-cleavage enzyme system of Escherichia coli.  Gene.  1986. 44(2-3):219-26.
[Link]

Urbanowski M, Stauffer G.  The metH gene from Salmonella typhimurium LT2: cloning and initial characterization.  Gene.  1986. 44(2-3):211-7.
[Link]

Neuwald A, Stauffer G.  DNA sequence and characterization of the Escherichia coli serB gene.  Nucleic Acids Res.  1985 October 11. 13(19):7025-39.
[Link]

Urbanowski M, Stauffer G.  Nucleotide sequence and biochemical characterization of the metJ gene from Salmonella typhimurium LT2.  Nucleic Acids Res.  1985 February 11. 13(3):673-85.
[Link]

Plamann M, Stauffer G.  Characterization of a cis-acting regulatory mutation that maps at the distal end of the Escherichia coli glyA gene.  J Bacteriol.  1985 February. 161(2):650-4.
[Link]

Park Y, Stauffer G.  Cloning and initial characterization of the metC gene from Salmonella typhimrium LT2.  Genetics.  1985. 110:S11.

Urbanowski M, Stauffer G.  Cloning and initial characterization of the metJ and metB genes from Salmonella typhimurium LT2.  Gene.  1985. 35(1-2):187-97.
[Link]

Neuwald A, Stauffer G.  The Escherichia coli serB gene may be part of an operon.  Genetics.  1985. 110:S11.

Schulte L, Stauffer L, Stauffer G.  Cloning and characterization of the Salmonella typhimurium metE gene.  J Bacteriol.  1984 June. 158(3):928-33.
[Link]

Plamann M, Stauffer L, Stauffer G.  A cis-acting regulatory mutation that maps at the distal end of the Escherichia coli glyA gene.  Genetics.  1984. 107:S83.

Schulte L, Stauffer G.  Cloning and characterization of the Salmonella typhimurium metE gene.  Abstr Ann Meet Amer Soc Microbiol.  1984. 

Urbanowski M, Stauffer G.  Cloning and initial characterization of the metJ repressor gene from Salmonella typhimurium.  Genetics.  1984. 107:S109-S110.

Garnant M, Stauffer G.  Construction and analysis of plasmids containing the Escherichia coli serB gene.  Mol Gen Genet.  1984. 193(1):72-5.
[Link]

Schulte L, Stauffer G.  In vitro fusion of the Salmonella typhimurium metE regulatory region to the lac operon.  Genetics.  1984. 107:S96.

Urbanowski M, Plamann M, Stauffer L, Stauffer G.  Cloning and characterization of the gene for Salmonella typhimurium serine hydroxymethyltransferase.  Gene.  1984 January. 27(1):47-54.
[Link]

Plamann M, Stauffer L, Urbanowski M, Stauffer G.  Complete nucleotide sequence of the E. coli glyA gene.  Nucleic Acids Res.  1983 April 11. 11(7):2065-75.
[Link]

Plamann M, Stauffer G.  Characterization of the Escherichia coli gene for serine hydroxymethyltransferase.  Gene.  1983 April. 22(1):9-18.
[Link]

Urbanowski M, Plamann M, Stauffer G.  Comparison of the regulatory regions of the glyA genes from Escherichia coli and Salmonella typhimurium.  Abstr Ann Meet Amer Soc Microbiol.  1983. 

Plamann M, Rapp W, Stauffer G.  Escherichia coli K12 mutants defective in the glycine cleavage enzyme system.  Mol Gen Genet.  1983. 192(1-2):15-20.
[Link]

Plamann M, Stauffer L, Stauffer G.  Isolation of an Escherichia coli mutant with altered levels of serine hydroxymethyl-transferase activity.  Abstr Ann Meet Amer Soc Microbiol.  1983. 

Stauffer G.  Regulation of serine, glycine, and one-carbon biosynthesis (Chapter 6).  1983. 

Stauffer G, Plamann M, Rapp W.  Genetic analysis of the glycine cleavage enzyme system of Escherichia coli.  Genetics.  1982. 100:S66.

Stauffer G, Plamann M, Stauffer L.  Construction and expression of hybrid plasmids containing the Escherichia coli glyA genes.  Gene.  1981. 14(1-2):63-72.
[Link]

Plamann M, Stauffer G.  In vitro fusion of the Escherichia coli glyA regulatory region to the lac operon.  Genetics.  1981. 97:S86.

Plamann M, Yeggy J, Stauffer G.  Cloning and characterization of the glyA gene from Escherichia coli K12.  Abstr Ann Meet Amer Soc Microbiol.  1980. 

Crawford I, Stauffer G.  Regulation of tryptophan biosynthesis.  Annu Rev Biochem.  1980. 49:163-95.
[Link]

Plamann M, Stauffer L, Stauffer G.  Structural and functional analysis of cloned deoxyribonucleic acid containing the glyA gene of the Escherichia coli chromosome.  Genetics.  1980. 94:S82.

Stauffer G, Zurawski G, Bennett G.  In vivo cloning of DNA regions carrying mutations linked to selectable genes: application to mutations in the regulatory region of the Escherichia coli tryptophan operon.  Plasmid.  1979 July. 2(3):498-502.
[Link]

Zurawski G, Elseviers D, Stauffer G, Yanofsky C.  Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon.  Proc Natl Acad Sci U S A.  1978 December. 75(12):5988-92.
[Link]

Stauffer G, Zurawski G, Yanofsky C.  Single base-pair alterations in the Escherichia coli trp operon leader region that relieve transcription termination at the trp attenuator.  Proc Natl Acad Sci U S A.  1978 October. 75(10):4833-7.
[Link]

Stauffer G, Brenchley J.  Selection of Salmonella typhimurium mutants with altered serine transhydroxymethylase regulation.  Genetics.  1978 February. 88(2):221-33.
[Link]

Stauffer G, Brenchley J.  Influence of methionine biosynthesis on serine transhydroxymethylase regulation in Salmonella typhimurium LT2.  J Bacteriol.  1977 February. 129(2):740-9.
[Link]

Stauffer G, Brenchley J.  Mutations altering serine transhydroxymethylase levels in Salmonella typhimurium.  Abstr Ann Meet Amer Soc Microbiol.  1975. 

Stauffer G, Baker C, Brenchley J.  Regulation of serine transhydroxymethylase activity in Salmonella typhimurium.  J Bacteriol.  1974 December. 120(3):1017-25.
[Link]

Stauffer G, Brenchley J.  Evidence for the involvement of serine transhydroxymethylase in serine and glycine interconversions in Salmonella typhimurium.  Genetics.  1974 June. 77(2):185-98.
[Link]

Stauffer G, Brenchley J.  Regulation of serine transhydroxymethylase activity in Salmonella typhimurium.  Abstr Ann Meet Amer Soc Microbiol.  1974. 

Date Last Modified: 07/09/2014 - 08:55:28