Microbiology

Linda L. McCarter, PhD

Portrait

Professor of Microbiology

Contact Information

Office: 3-430 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
Office Phone: 319-335-9721

Lab: 3-401 Bowen Science Building
51 Newton Rd
Iowa City, IA 52242
Phone: 319-353-5421

Email: linda-mccarter@uiowa.edu

Education

BA, Chemistry, University of Hawaii, Honolulu
PhD, Microbiology, University of California, Davis

Post Doctoral, Microbiology, The Agouron Institute, La Jolla, CA

Education/Training Program Affiliations

Biosciences Graduate Program
Department of Microbiology Graduate Program
Interdisciplinary Graduate Program in Translational Biomedicine
Medical Scientist Training Program

Research Summary

In order to survive in changing environments, bacteria possess enormous adaptive capabilities that allow them to modulate their behavior and reprogram gene expression in response to environmental cues. One important survival strategy for bacteria is adhesion to and colonization of surfaces. My laboratory is focused on understanding how bacteria adapt to life on surfaces and in biofilms. We ask the following kinds of questions. How do bacteria recognize surfaces? What genes are turned on by growth on a surface? How is behavior coordinated? How do bacteria interact to develop surface-attached communities or biofilms? How do bacteria process information and make decisions-for example, make the decision to swim, swarm or stick? We also are probing the significance of surface sensing with respect to virulence of the organism. We study Vibrio parahaemolyticus, a worldwide pathogen and a model organism for surface sensing-as it undergoes the remarkable differentiation known as swarming when transitioning from liquid to surfaces. Our ultimate goal is to define the gene activity pertinent to growth on surfaces and to trace the regulatory circuitry that enable bacteria to establish growth, coordinate behavior, and develop structured communities on surfaces. How cells program gene expression when growing as communities on surfaces may be key to understanding pathogenesis of many organisms.

Specific projects:

1. Surface sensing, signal transduction and motility

2. Genetic control of growth on surfaces: cyclic di-GMP and GGDEF-EAL circuitry

3. Biofilm development and regulation of cell surface molecules such as polysaccharide

4. Cell-cell signaling and communication

5. Transcriptional profiling

6. Mechanisms of virulence

All Publications

Jensen R, Depasquale S, Harbolick E, Hong T, Kernell A, Kruchko D, Modise T, Smith C, McCarter L, Stevens A.  Complete Genome Sequence of Prepandemic Vibrio parahaemolyticus BB22OP.  Genome Announc.  2013 January. 1(1):pii: e00002-12.
[Link]

Ferreira R, Chodur D, Antunes L, Trimble M, McCarter L.  Output targets and transcriptional regulation by a cyclic dimeric GMP-responsive circuit in the Vibrio parahaemolyticus Scr network.  J Bacteriol.  2012 March. 194(5):914-24.
[Link]

Trimble M, McCarter L.  Bis-(3'-5')-cyclic dimeric GMP-linked quorum sensing controls swarming in Vibrio parahaemolyticus.  Proc Natl Acad Sci U S A.  2011 November 1. 108(44):18079-84.
[Link]

Gode-Potratz C, McCarter L.  Quorum sensing and silencing in Vibrio parahaemolyticus.  J Bacteriol.  2011 August. 193(16):4224-37.
[Link]

Matz C, Nouri B, McCarter L, Martinez-Urtaza J.  Acquired type III secretion system determines environmental fitness of epidemic Vibrio parahaemolyticus in the interaction with bacterivorous protists.  PLoS One.  2011. 6(5):e20275.
[Link]

Gode-Potratz C, Kustusch R, Breheny P, Weiss D, McCarter L.  Surface sensing in Vibrio parahaemolyticus triggers a programme of gene expression that promotes colonization and virulence.  Mol Microbiol.  2011 January. 79(1):240-63.
[Link]

Gode-Potratz C, Chodur D, McCarter L.  Calcium and iron regulate swarming and type III secretion in Vibrio parahaemolyticus.  J Bacteriol.  2010 November. 192(22):6025-38.
[Link]

McCarter L.  Bacterial acrobatics on a surface: swirling packs, collisions, and reversals during swarming.  J Bacteriol.  2010 July. 192(13):3246-8.
[Link]

McCarter L.  The Scr circuit in Vibrio parahaemolyticus modulates swarming and sticking.  ASM Press, Washington, DC.  2010. 

Ferreira R, Antunes L, Greenberg E, McCarter L.  Vibrio parahaemolyticus ScrC modulates cyclic dimeric GMP regulation of gene expression relevant to growth on surfaces.  J Bacteriol.  2008 February. 190(3):851-60.
[Link]

Kim Y, McCarter L.  ScrG, a GGDEF-EAL protein, participates in regulating swarming and sticking in Vibrio parahaemolyticus.  J Bacteriol.  2007 June. 189(11):4094-107.
[Link]

McCarter L.  Regulation of flagella.  Curr Opin Microbiol.  2006 April. 9:180-6.
[Link]

Jaques S, McCarter L.  Three new regulators of swarming in Vibrio parahaemolyticus.  J Bacteriol.  2006 April. 188(7):2625-35.
[Link]

McCarter L.  Motility and chemotaxis.  ASM Press, Washington, DC.  2006. 

Enos-Berlage J, Guvener Z, Keenan C, McCarter L.  Genetic determinants of biofilm development of opaque and translucent Vibrio parahaemolyticus.  Mol Microbiol.  2005 February. 55(4):1160-82.
[Link]

McCarter L.  Multiple modes of motility: a second flagellar system in Escherichia coli.  J Bacteriol.  2005 February. 187(4):1207-9.
[Link]

Trosky J, Mukherjee S, Burdette D, Roberts M, McCarter L, Siegel R, Orth K.  Inhibition of MAPK signaling pathways by VopA from Vibrio parahaemolyticus.  J Biol Chem.  2004 December 10. 279(50):51953-7.
[Link]

Doyle T, Hawkins A, McCarter L.  The complex flagellar torque generator of Pseudomonas aeruginosa.  J Bacteriol.  2004 October. 186(19):6341-50.
[Link]

Kim Y, McCarter L.  Cross-regulation in Vibrio parahaemolyticus: compensatory activation of polar flagellar genes by the lateral flagellar regulator LafK.  J Bacteriol.  2004 June. 186(12):4014-8.
[Link]

McCarter L.  Dual flagellar systems enable motility under different circumstances.  J Mol Microbiol Biotechnol.  2004. 7:18-29.
[Link]

Güvener Z, McCarter L.  Multiple regulators control capsular polysaccharide production in Vibrio parahaemolyticus.  J Bacteriol.  2003 September. 185(18):5431-41.
[Link]

Stewart B, McCarter L.  Lateral flagellar gene system of Vibrio parahaemolyticus.  J Bacteriol.  2003 August. 185(15):4508-18.
[Link]

Boles B, McCarter L.  Vibrio parahaemolyticus scrABC, a novel operon affecting swarming and capsular polysaccharide regulation.  J Bacteriol.  2002 November. 184(21):5946-54.
[Link]

McCarter L.  Polar flagellar motility of the Vibrionaceae.  Microbiol Mol Biol Rev.  2001 September. 65:445-62, table of contents.
[Link]

Gestwicki J, Lamanna A, Harshey R, McCarter L, Kiessling L, Adler J.  Evolutionary conservation of methyl-accepting chemotaxis protein location in Bacteria and Archaea.  J Bacteriol.  2000 November. 182(22):6499-502.
[Link]

Enos-Berlage J, McCarter L.  Relation of capsular polysaccharide production and colonial cell organization to colony morphology in Vibrio parahaemolyticus.  J Bacteriol.  2000 October. 182(19):5513-20.
[Link]

Kim Y, McCarter L.  Analysis of the polar flagellar gene system of Vibrio parahaemolyticus.  J Bacteriol.  2000 July. 182(13):3693-704.
[Link]

Boles B, McCarter L.  Insertional inactivation of genes encoding components of the sodium-type flagellar motor and switch of Vibrio parahaemolyticus.  J Bacteriol.  2000 February. 182(4):1035-45.
[Link]

McCarter L.  Surface-induced gene expression in Vibrio parahaemolyticus.  Horizon Scientific Press, Norfolk, England.  2000. 

McCarter L.  The multiple identities of Vibrio parahaemolyticus.  J Mol Microbiol Biotechnol.  1999 August. 1:51-7.
[Link]

Jaques S, Kim Y, McCarter L.  Mutations conferring resistance to phenamil and amiloride, inhibitors of sodium-driven motility of Vibrio parahaemolyticus.  Proc Natl Acad Sci U S A.  1999 May 11. 96(10):5740-5.
[Link]

McCarter L.  OpaR, a homolog of Vibrio harveyi LuxR, controls opacity of Vibrio parahaemolyticus.  J Bacteriol.  1998 June. 180(12):3166-73.
[Link]

Stewart B, Enos-Berlage J, McCarter L.  The lonS gene regulates swarmer cell differentiation of Vibrio parahaemolyticus.  J Bacteriol.  1997 January. 179(1):107-14.
[Link]

Kawagishi I, Imagawa M, Imae Y, McCarter L, Homma M.  The sodium-driven polar flagellar motor of marine Vibrio as the mechanosensor that regulates lateral flagellar expression.  Mol Microbiol.  1996 May. 20(4):693-9.
[Link]

Stewart B, McCarter L.  Vibrio parahaemolyticus FlaJ, a homologue of FliS, is required for production of a flagellin.  Mol Microbiol.  1996 April. 20(1):137-49.
[Link]

McCarter L.  Genetic and molecular characterization of the polar flagellum of Vibrio parahaemolyticus.  J Bacteriol.  1995 March. 177(6):1595-609.
[Link]

McCarter L.  MotX, the channel component of the sodium-type flagellar motor.  J Bacteriol.  1994 October. 176(19):5988-98.
[Link]

McCarter L.  MotY, a component of the sodium-type flagellar motor.  J Bacteriol.  1994 July. 176(14):4219-25.
[Link]

McCarter L, Wright M.  Identification of genes encoding components of the swarmer cell flagellar motor and propeller and a sigma factor controlling differentiation of Vibrio parahaemolyticus.  J Bacteriol.  1993 June. 175(11):3361-71.
[Link]

Atsumi T, McCarter L, Imae Y.  Polar and lateral flagellar motors of marine Vibrio are driven by different ion-motive forces.  Nature.  1992 January 9. 355(6356):182-4.
[Link]

Silverman M, Showalter R, McCarter L.  Genetic analysis in vibrio.  Methods Enzymol.  1991. 204:515-36.
[Link]

McCarter L, Silverman M.  Surface-induced swarmer cell differentiation of Vibrio parahaemolyticus.  Mol Microbiol.  1990 July. 4:1057-62.
[Link]

Sar N, McCarter L, Simon M, Silverman M.  Chemotactic control of the two flagellar systems of Vibrio parahaemolyticus.  J Bacteriol.  1990 January. 172(1):334-41.
[Link]

McCarter L, Silverman M.  Iron regulation of swarmer cell differentiation of Vibrio parahaemolyticus.  J Bacteriol.  1989 February. 171(2):731-6.
[Link]

McCarter L, Hilmen M, Silverman M.  Flagellar dynamometer controls swarmer cell differentiation of V. parahaemolyticus.  Cell.  1988 July 29. 54(3):345-51.
[Link]

McCarter L, Silverman M.  Phosphate regulation of gene expression in Vibrio parahaemolyticus.  J Bacteriol.  1987 August. 169(8):3441-9.
[Link]

Simon M, Belas R, McCarter L, Bartlett D, Silverman M.  Microbial role of biodeterioration.  American Institute of Biological Sciences, Oxford, and IBH Publishing, New Delhi, India.  1987. 

Belas R, McCarter L, Simon M, Silverman M.  Recognition of surfaces by Vibrio parahaemolyticus .  Proceedings of the 3rd Annual MIT Sea Grant Lecture, Hemisphere, NY.  1985. 

McCarter L, Krajewska-Grynkiewicz K, Trinh D, Wei G, Kustu S.  Characterization of mutations that lie in the promoter-regulatory region for glnA, the structural gene encoding glutamine synthetase.  Mol Gen Genet.  1984. 197(1):150-60.
[Link]

McFarland N, McCarter L, Artz S, Kustu S.  Characterization of λglnA + phages used as templates for in vitro synthesis of glutamine synthetase.  Mol Gen Genet.  1982 March. 185(1):152-7.

McFarland N, McCarter L, Artz S, Kustu S.  Nitrogen regulatory locus "glnR" of enteric bacteria is composed of cistrons ntrB and ntrC: identification of their protein products.  Proc Natl Acad Sci U S A.  1981 April. 78(4):2135-9.
[Link]

Kustu S, Burton D, Garcia E, McCarter L, McFarland N.  Nitrogen regulation in Salmonella typhimurium.  Academic Press, New York.  1980. 

Kustu S, Burton D, Garcia E, McCarter L, McFarland N.  Nitrogen control in Salmonella: regulation by the glnR and glnF gene products.  Proc Natl Acad Sci U S A.  1979 September. 76(9):4576-80.
[Link]

Date Last Modified: 06/07/2014 - 21:56:23