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Associate Professor of Microbiology
Office: 3-772 Bowen Science Building51 Newton RdIowa City, IA 52242
Lab: 3-701 Bowen Science Building51 Newton RdIowa City, IA 52242
MD, Medicine, China Medical UniversityMS, Biochemistry, China Medical UniversityPhD, Biochemistry, Hamamatsu University School of Medicine
Post Doctorate, Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health
Department of Microbiology Graduate ProgramInterdisciplinary Graduate Program in ImmunologyMedical Scientist Training Program
The identity of a cell type is determined by genes it expresses, where transcriptional regulation plays a central role. Differentiation of hematopoietic stem cells (HSCs) to antigen responding T cells is accompanied by many stages of cell identity transition. Our lab aims to decode how key transcription factors maintain the cell identity and instigate the transitions during the following biological processes:
1. self-renewal of HSCs to maintain a constant supply of multiple blood lineages throughout lifetime, and how aging affects HSC maintenance and multipotency;
2. T cell development in the thymus from immature progenitors, and how developing thymocytes, which are highly proliferative and are accompanied by constant DNA break and repair during the recombination process, are safeguarded from malignant transformation; and
3. transition of antigen-specific effector T cells to memory T cells to form stable immunological memory.
Our current focus is on two groups of transcription factors, GABP and TCF-1/LEF-1. GABP (GA binding protein) subunits and isoforms demonstrated relative stable expression in all the processes described above, however, along with the identity transition, GABP adapts well and assumes different roles. On the other hand, TCF-1/LEF-1 expression and activity are regulated in response to extracellular Wnt signaling. We are using molecular biology, genomic, proteomic, and bioinformatic approaches to decipher the interplay of these key transcription factors in these hematological/immunological processes.
Center for Immunology and Immune-based DiseasesHolden Comprehensive Cancer Center
TCF-1 upregulation identifies early innate lymphoid progenitors in the bone marrow.
2015 October. 16(10):1044-50.
LEF-1 and TCF-1 orchestrate TFH differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6.
2015 September. 16(9):980-90.
TCF-1 and LEF-1 act upstream of Th-POK to promote the CD4(+) T cell fate and interact with Runx3 to silence Cd4 in CD8(+) T cells.
2014 July. 15(7):646-56.
The TCF-1 and LEF-1 Transcription Factors Have Cooperative and Opposing Roles in T Cell Development and Malignancy.
2012 November 16. 37(5):813-26.
Targeting tetramer-forming GABPβ isoforms impairs self-renewal of hematopoietic and leukemic stem cells.
Cell Stem Cell.
2012 August 3. 11(2):207-19.
Regulation of mature T cell responses by the Wnt signaling pathway.
Ann N Y Acad Sci.
2012 January. 1247:16-33.
GABP controls a critical transcription regulatory module that is essential for maintenance and differentiation of hematopoietic stem/progenitor cells.
2011 February 17. 117(7):2166-78.
Differentiation and persistence of memory CD8(+) T cells depend on T cell factor 1.
2010 August 27. 33(2):229-40.
The transcription factor GABP is a critical regulator of B lymphocyte development.
2007 April. 26(4):421-31.
GA binding protein regulates interleukin 7 receptor alpha-chain gene expression in T cells.
2004 October. 5(10):1036-44.
Date Last Modified: 10/09/2015 -
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