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Address: 3-730 BSB
Phone: (319) 335-7576
Mentor: Stanley Perlman
Undergraduate Institution: University of Nebraska-Lincoln
PhD Institution: Washington University-St Louis
I am interested in the role of the ADRP (ADP-ribose 1' phosphatase) domain encoded within nsp3 of both Severe Acute Respiratory Syndrome (SARS)-Coronavirus and Murine Hepatitis Virus (MHV). This domain is known to have enzymatic activity and binds ADP-ribose however it is still unclear how these activities would affect viral replication and/or pathogenesis. It has previously been shown that this domain is important for pathology of the liver during MHV infection, however its role in brain pathology caused by MHV or during SARS infection of mice has not yet been examined. We are using a variety of novel genetic tools including Bacterial Artificial Chromosome (BAC) technology to create specific point mutations within the ADRP domain and address these questions. Ultimately we hope to identify the host proteins which interact with this domain and the mechanism it uses to cause pathogenesis.
Fehr A.R. and D. Yu. 2010. Human cytomegalovirus gene UL21a encodes a short-lived cytoplasmic protein and facilitates virus replication in fibroblasts. J Virol. 84:291-302.
Fuchs E.L., E.D. Brutinel, E.R. Klem, A.R. Fehr, T.L. Yahr, and M.C. Wolfgang. 2010. In vitro and in vivo characterization of the Pseudomonas aeruginosa cyclic AMP (cAMP) phosphodiesterase CpdA, required for cAMP homeostasis and virulence factor regulation. J Bacteriol. 192(11):2779-90.
Fehr A.R. and D. Yu. 2011. Human cytomegalovirus early protein pUL21a facilitates efficient viral DNA synthesis and the late accumulation of immediate-early transcripts. J Virol. 85:663-674.
Perng Y., Z. Qian, A.R. Fehr, B. Xuan, and D. Yu. 2 March 2011. Human cytomegalovirus gene UL79 is required for the accumulation of late viral transcripts. J Virol. doi:10.1128/JVI.02344-10
Fehr A.R., Gualberto N.C., Savaryn J.P., Terhune S.S., and Yu D. (2012) Proteasome-Dependent Disruption of the E3 Ubiquitin Ligase Anaphase-Promoting Complex by HCMV Protein pUL21a. PLoS Pathog 8(7): e1002789. doi:10.1371/journal.ppat.1002789
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