Protein Crystallography Facility

  • Services

    Lokesh and Rams in Lab

    • Assistance with protein purification
    • Protein purity analysis by dynamic light scattering
    • Molecular weight determination by static light scattering
    • Set-up of crystallization screens using commercial and facility-made conditions
    • Preliminary x-ray screening of crystals
    • Data collection of quality crystals at facility x-ray source and synchrotrons
    • Data processing using d*TREK, XDS and mosflm
    • Structure determination using MR, SAD, MAD, MIR techniques with ccp4, Phenix, Phaser, SHELX, SHARP/autoSHARP
    • Structure refinement and fitting using Refmac, Phenix, Coot
    • Structure visualization and analysis using PyMOL, VMD, Chimera
    • Homology modeling and docking
    • SAXS data collection, processing and modeling