Protein Crystallography Facility

  • Facility Instrumentation

    The Crystallography Facility offers a wide variety of instrumentation including facilities for x-ray data collection, light scattering, crystallization and data manipulation.

    Size Exclusion Chromatography

     BioRad Duo Flow

    Three Bio-Rad DuoFlow FPLC systems at 4C and room temperature with Superdex 75, Superdex 200, Superose 6, Sepharcryl S-200 columns from GE

     

    Light Scattering         

    DynaPro Nanostar 

    DynaPro NanoStar (Wyatt Technology) with additional static light scattering detector and temperature control

     
    DynaPro

    DynaPro (Protein Solutions/Wyatt Technology) with temperature control for dynamic light scattering

     

    Crystallization Instruments

     Tecan Genesis

    Tecan Genesis liquid handler RSP 150/8 for dispersing commercial crystallization well solutions

                     
     Rigaku Alchemist

    Rigaku Alchemist for setting up customized crystallization well solutions

     
     Imaging System

    Formulatrix RockImager system for visualizing crystallization drops post set-up

     
     TTP LabTech Mosquito

    TTP LabTech Mosquito for setting up crystallization drops

     

    X-ray Instrumentation

     Rigaku Xray Generators

    2 Rotating anode (RUH3R) Rigaku generators (1.5µÅ)

     
     Rigaku Xray Detectors

    2 R-AXIS IV++ Rigaku x-ray detectors

     
     Cryostream

    Rigaku X-stream and Oxford Cryostream 700 for cryogenic (100K) data collection

     
     4DX Microspectrophotometer

    4DX Systems Microspectrophotometer for absorbtion measurements on single crystals

     

    Synchrotron Access

    Berkeley Synchrotron

    Advanced Light Source

    The Carver College of Medicine has invested a 1/10 share in the 4.2.2 synchrotron beam line located at the Advanced Light Source in the Ernest Orlando Lawrence Berkeley National Laboratory. The synchrotron is tuned to produce high intensity x-ray radiation and the beam line end station is set up to collect x-ray diffraction data to determine three-dimensional structures of proteins that are hard to carry out at home. Reserved time is available at this facility and enables researchers to remotely perform crystallographic structure determinations faster at much higher quality.

    Data Workstations and Storage

     Workstation

    Linux-based workstations with stereo 3D capability for processing and visualization are available for investigator use.

     
     Netgear ReadyNAS

    Netgear ReadyNAS X-Raid2 dual redundant file storage for all data collected

     

    Projection

     Projector

    VR-Dual 1000 stereoscopic 3-D projector system for presentations and teaching