Bovine C_HNomenclature[ 13-11-02]

Original Description	Current Designator	Chain & Allotype	Gene	Serological Allele	Sequence Allele	GenBank Acct. No.	Major Features and Other Information
IgG1	IgG1^a IgG1^b	γ1^a γ1^b	IGHG1 IGHG1		G1 * 01 G1 * 02		Hinge, Arg218; Thr226 Hinge; Thr218; Pro224; Pro226
IgG2a(A1) IgG2a(A2)	IgG2^a IgG2^b	γ2^a γ2^b	IGHG2 IGHG2	G2^a G2^b	G2 + 01 G2 + 02	X16702 S82407	CH3; intradomain loop hepatappetide; Arg419 Middle hinge, CH3, Glu419
IgG2b/IgG3	IgG3^a IgG3^b	γ3^a γ3^b	IGHG3 IGHG3		G3 * 01 G3 * 02	U63638 U63639	37 amino acid hinge 6 amino acid substitutions; 84 bp, INV3 insertion
IgA	IgA	a	IGHA			AF109167	PstI RFLP generates polynucleotides of 6.0 and 6.3 kb suggestive of sequence alleles
IgM	IgM	μ	IGHM			U63637
IgE	IgE	ε	IGHE			U63640	PstI, EcoRI and BAMI RFLPs show polymorphism suggestive of sequence alleles
IgD	IgD	δ	IGHD			AF515672 and AF411240	mCH1 like dCH1; transcribed through class switch recombination directed by the Sd

References

Blakeslee, D., J. E. Butler, and W. H. Stone. 1971. Serum antigens of cattle. II. Immunogenetics of two immunoglobulin allotypes. J. Immunol. 107:227-235.

Brown, W. R., H. H. Rabbani, J. E. Butler and L. Hammarström. 1997. Characterization of a bovine Ca gene. Immunology (British) 91:1-6.

Butler, J. E. 1983. Bovine immunoglobulins: An augmented review. Vet. Immunol. & Immunopath. (Adv. Vet. Immunol.) 4:43-152.

Butler, J. E. 1985. Biochemistry and biology of ruminant immunoglobulins. Prog. Vet. Microbiol. Immun. Vol. 2, pp. 1-53 (R. Pandey, Ed.), Karger, Basel.

Butler, J.E. 1998 Immunoglobulin diversity, B-cell and antibody repertoire development in large farm animals. OIE Scientific and Technical Review 17 :43-70

Heyermann, H., and J. E. Butler. 1987. The heterogeneity of bovine IgG2. IV. Structural differences between IgG2a bearing the A1 and A2 allotypic determinants. Molec. Immunol. 24:1327-1334.

Heyermann, H., J. E. Butler and B. Frangione. 1992. The heterogeneity of bovine IgG2. V. Differences in the primary structure of bovine IgG allotypes. Mol. Immunol. 29:1147-1152.

Jackson, T., B. A. Morris, and P. G. Sanders. 1992. Nucleotide sequences and expression of cDNAs for a bovine anti-testosterone monoclonal IgG1 antibody. Mol. Immunol. 29:667-676.

Kacskovics, I., and J. E. Butler. 1996. The heterogeneity of bovine IgG2. VIII. The complete cDNA sequence of bovine IgG2a(A2) and an IgG1. Molec. Immunol. 33:189-195.

Knight, K.L., M. Suter and R.S. Becker. 1989. Genetic engineering of bovine Ig. Construction and characterization of hapten-binding bovine/murine chimeric IgE,IgA,IgG1,IgG2 and IgG3 molecules. J. Immunol. 140:3654-3659.

Mousavi, M., H. Rabbani and L. Hammarström. 1997. Characterization of the bovine epsilon gene. Immunology. 92:369-373.

Mousavi, M., H. Rabbani, L. Pilstrom and L. Hammarström. 1998. Characterization of the gene for the membrane and secretory form of the IgM heavy-chain constant region gene (Cm) for the cow (Bos taurus). Immunology. 93:581-588.

Rabbani, H., W. R. Brown, J. E. Butler and L. Hammarström. 1997. Polymorphism of the IgHG3 gene in cattle. Immunogenetics. 46:326-331.

Saini, S. S., W. Farrugia, N. Muthusamy, P. A. Ramsland, and A. K. Kaushik. 2007. Structural evidence for a new IgG1 antibody sequence allele of cattle. Scand. J. Immunol. 65:32-38.

Symons, D. B., C. A. Clarkson, and D. Beale. 1989. Structure of bovine immunoglobulin constant region heavy chain gamma 1 and gamma 2 genes. Mol. Immunol. 26:841-850.

Zhao , Y., I. Kacskovics, Q. Pan, D. A. Liberles, J. Geli, S. K. Davis, H. Rabbani, and L. Hammarström. 2002. Artiodactyl IgD: the missing link. J. Immunol. 169:4408-4416.

Zhao, Y., I. Kacskovics, H. Rabbani, and L. Hammarström. 2003. Physical mapping of the bovine immunoglobulin heavy chain constant region gene locus. J. Biol. Chem. 278:35024-35032.

1) Curators S. S. Saini and A. Kaushik with input from R. Goldsby, Barbara A. Osborne and R. Aitkin.

Parng C. L., Hansal, S., Goldsby, R. A., Osborne BA. 1996. J Immunol. 157:5478-86.

References

Saini, S. S., Hein, W. and Kaushik, A. 1997. A single predominantly expressed polymorphic immunoglobulin V_H gene family related to mammalian group I, clan II, is identified in cattle. Molecular Immunology. 34:641-51.

Jackson T, Morris BA, Sanders PG. 1992. Nucleotide sequences and expression of cDNAs for a bovine anti-testosterone monoclonal IgG1 antibody. Mol Immunol.29:667-76

Current Designation		Proposed Designation		Prototypic Gene	Sequence Allele	GenBank Acc. # / Reference	Comments
Family	Subfamily	Family	Subfamily	Prototypic Gene	Sequence Allele	GenBank Acc. # / Reference	Comments
V_l1	V_l1a	V_l1	V_l1a	BLV4H9	-	AF015796	Predominantly expressed gene subfamily
	V_l1b		V_l1b	BLV7B7	-	AF015803	Predominantly expressed gene subfamily
	V_l1c		V_l1c	Clone 15	-	Sinclair et al 1995	Rarely expressed
	V_l1x		V_l1x	BLV1H12	-	AF023843	Preferential pairing with VDJ rearrangement encoding exceptionally long CDR3H
	V_l1d		V_l1d	BLV8C11	-	AF023842	Preferential pairing with VDJ rearrangement encoding exceptionally long CDR3H
	V_l1e		V_l1e	BF1H1	-	AF097214	Preferential pairing with VDJ rearrangement encoding exceptionally long CDR3H
V_l2	-	V_l2	V_l2	Clone 13	-	Sinclair et al 1995	Rarely expressed gene family
?	-	V_l3	V_l3	Clone C4	-	Parng et al 1996	Rarely expressed gene family

Current Description	Proposed Designation	Gene	Serological Allele	Sequence Allele	GenBank Acc. #	Major Features and Other Information
Bov V_H1	Bov V_H1	IGHV4-pB7S2			U36823
Bov V_H1	Bov V_H1	IGHV4-pB8S1			U36824
Bov V_H1	Bov V_H1	IGHV4-p2C7.5			AF000012
Bov V_H1	Bov V_H1	IGHV4-p4H9.1			AF000013
Bov V_H1	Bov V_H1	IGHV4-p5H4.1			AF000014
Bov V_H1	Bov V_H1	IGHV4-p7B7.2			AF000015
Bov V_H1	Bov V_H1	IGHV4-p7G1.5			AF000016
Bov V_H1	Bov V_H1	IGHV4-1H12			AF015506	Preferential use in VDJ rearrangements encoding exceptionally long CDR3H (up to 61 amino acids)
Bov V_H1	Bov V_H1	IGHV4-A7M			U49772
Bov V_H1	Bov V_H1	IGHV4-A10M			U49773
Bov V_H1	Bov V_H1	IGHV4-B11			U11629
Bov V_H1	Bov V_H1	IGHV4-PUC13.8.75			X62916

Bovine CH Nomenclature[ 13-11-02]

References

Family

References

Bovine C_HNomenclature[ 13-11-02]