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Nomenclature:

Camelid CH Nomenclature1       [07-19-2002]  

Dromedary

Original Description

Current Designation

Chain & Allotype

Gene Allele

Serological Allele

Sequence Allele

GenBank Acc. #

Major Features and Other Information

IgG1a

IgG1A

γ1a

IGHG1A

 

H2-L2 composition, binds protein A and protein G, sequence only obtained as cDNA

IgG1b

IgG1B

γ1b

IGHG1B

 

H2-L2 composition, binds protein A and protein G, sequence only obtained as cDNA

IgG2a

IgG2A

γ2a

IGHG2A

AJ131945

Heavy-chain antibody devoid of L chains, binds protein A, not protein G.  Sequence obtained as cDNA and from cloned genomic DNA

IgG2c

IgG2C

γ2c

IGHG2C

 

 

Heavy-chain antibody devoid of L chains, binds protein A, not protein G.  Sequence only obtained as cDNA.

IgGP1

Ψγ

IGHGP1

 

Pseudogene ?, sequence found in clones of genomic DNA, never as cDNA.  Largest homology with IGHG2C.

IgGP2

Ψγ

IGHGP2

Pseudogene ?, sequence found in clones of genomic DNA, never as cDNA.  Largest homology with IGHG2C.

IgGP3

Ψγ

IGHGP3

Pseudogene ?, sequence found in clones of genomic DNA, never as cDNA.  Largest homology with IGHG2C.

IgGP4

Ψγ

IGHGP4

Pseudogene ?, sequence found in clones of genomic DNA, never as cDNA.  Largest homology with IGHG2C.

IgG3

IgG3

γ3

IGHG3

Heavy-chain antibody devoid of L chains, binds protein A and protein G.  Sequence only obtained as cDNA.

IgM

IgM

μ

IGHM

 

Sequence obtained as cDNA, and 3' end of gene as cloned genomic DNA (Cμ4-exon)

 

Llama

Original Description

Current Designation

Chain & Allotype

Gene Allele

Serological Allele

Sequence Allele

GenBank Acc. #

Major Features and Other Information

IgG1a and B1

IgG1B

γ1a

IGHG1A

AF132603

H2-L2 composition, binds protein A and protein G, sequence only obtained as cDNA and as cloned genomic DNA.

IgG1b

IgG1B

γ1b

IGHG1B

AF305955

H2-L2 composition, binds protein A and protein G, sequence only obtained as cDNA and as cloned genomic DNA.

IgG2a

IgG2A

γ2a

IGHG2A

Heavy-chain antibody devoid of L chains, binds protein A, not protein G.  Sequence only obtained as cDNA.

IgG2b

IgG2B*01

γ2b*01

IGHG2B*01

 

AF132604

Heavy-chain antibody devoid of L chains, binds protein A, not protein G.  Sequence obtained as cloned genomic DNA only, two amino acids inserted in te hinge relative to γ2b*01 sequence.

IgG2c and B3

IgG2C

γ2c

IGHG2C

AF132605

Heavy-chain antibody devoid of L chains, binds protein A, not protein G.  Sequence obtained as cDNA and from cloned genomic DNA.

IgG3

IgG3

γ3

IGHG3

Heavy-chain antibody devoid of L chains, binds protein A, not protein G.  Sequence only obtained as cDNA.

 

1) Curator  Serge Muyldermans

 

References

 

Ngyuen, V.K., Hamers, R., Wyns, L., and Muyldermans, S.  1999.  Loss of splice consensus signal is responsible for the rmoval of the entire CH1 domain of the functional camel IgG2A heavy chain antibodies.  Molec. Immunol.  36:515-524.

Vu, K.B., Ghahroudi, M.A., Wyns, L., and Muyldermans, S.  1997.  Comparison of llama VH sequences from conventional and heavy chain antibodies.  Molec. Immunol.  34:1121-1131.

Woolven, B., Frenken, L., van der Logt, P., and Nicholls, P.J.  1999.  The structure of the llama heavy chain constant genes reveals a mechanism for heavy-chain antibody formation.  Immunogenetics  50:98-100.

 

 

 

 

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Camelid CL Nomenclature1     [03-14-2002] 

Dromedary

Original Description

Current Designation

Chain & Allotype

Gene

Serological Allele

Sequence Allele

GenBank Acc. #

Major Features and Other Information

Igλ1

Igλ1

λ1

IGLLλ

 

Igλ2

Igλ2

λ2

IGLLλ

 

Igκ

Igκ

κ

IGLκ

 

1)  Curator  Serge Muyldermans

 

References

Harmsen, M.M., Ruuls, R.C., Nijman, I.J., Niewold, T.A., Frenken, L, and de Geus, B.  (YEAR???).  Llamba heavy chain V-regions consist of at least four distinct subfamilies revealing novel sequence features.  Molec. Immunol.  37:579-590.

 

Nguyen, V.K., Hamers, R, Wyns, L., and Muyldermans, S.  2000.  Camel heavy-chain antibodies: diverse germline VHH and specific mechanisms enlarge the antigen-binding repertoire.  EMBO J 19:921-931. 

 

 

 

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 Dromedary VH Nomenclature1            [7/23/2002]

Current Description Proposed Designation Gene Serological Allele Sequence Allele GenBank Acc. # Major Features and Other Information
cvh1   IGHV1S1     AF000603 VH germline gene (encodes V37, G44, L45, W47)
cvhp01   IGHV1S1     AJ245149 Same sequence as cvh1 except for 1 missing nucleotide
cvhp02         AJ245150 More than 5 nucleotide difference with cvh1, cvhp01, cvhp13, cvhp14, cvhp15 clones but encodes an identical VH germline amino acid sequence
cvhp13         AJ245161 More than 5 nucleotide difference with cvh1, cvhp01, cvhp02, cvhp14, cvhp15 clones but encodes an identical VH germline amino acid sequence
cvhp14         AJ245162 More than 5 nucleotide difference with cvh1, cvhp01, cvhp02, cvhp13, cvhp15 clones but encodes an identical VH germline amino acid sequence
cvhp15         AJ245163 More than 5 nucleotide difference with cvh1, cvhp01, cvhp02, cvhp13, cvhp14 clones but encodes an identical VH germline amino acid sequence
cvhp03   IGHV1S2     AJ245151 VH germline gene (encodes V37, G44, L45, W47)
cvhp04   IGHV1S3     AJ245152 VH germline gene (encodes V37, G44, L45, W47)
cvhp05   IGHV1S4     AJ245153 VH germline gene (encodes V37, G44, L45, W47)
cvhp06   IGHV1S5     AJ245154 VH germline gene (encodes V37, G44, L45, W47)
cvhp07   IGHV1S6     AJ245155 VH germline gene (encodes V37, G44, L45, W47)
cvhp09   IGHV1S7     AJ245157 VH germline gene (encodes V37, G44, L45, W47)
cvhp10   IGHV1S8     AJ245158 VH germline gene (encodes V37, G44, L45, W47)
cvhp11   IGHV1S9     AJ245159 VH germline gene (encodes V37, G44, L45, W47)
cvhp12   IGHV1S10     AJ245160 VH germline gene (encodes V37, G44, L45, W47)
cvhp16   IGHV1S11     AJ245164 VH germline gene (encodes V37, G44, L45, W47)
cvhp17   IGHV1S12   IGHV1S12*01 AJ245165 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp18
cvhp18   IGHV1S12   IGHV1S12*02 AJ245166 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp17
cvhp19   IGHV1S13     AJ245167 VH germline gene (encodes V37, G44, L45, W47)
cvhp20   IGHV1S14   IGHV1S14*01 AJ245168 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp21
cvhp21   IGHV1S14   IGHV1S14*02 AJ245169 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp20
cvhp22   IGHV1S15     AJ245170 VH germline gene (encodes V37, G44, L45, W47)
cvhp23   IGHV1S16   IGHV1S16*01 AJ245171 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp24
cvhp24   IGHV1S16   IGHV1S16*02 AJ245172 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp23
cvhp25   IGHV1S17     AJ245173 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp23
cvhp26   IGHV1S18     AJ245174 VH germline gene (encodes V37, G44, L45, W47)
cvhp27         AJ245175 More than 5 nucleotide difference with clones cvhp26 and cvhp2á but encodes identical VH germline amino acid sequence
cvhp28         AJ245176 More than 5 nucleotide difference with clones cvhp26 and cvhp27 but encodes an indentical VH germline amino acid sequence
cvhp08   IGHV1S19     AJ245156 VH germline gene (encodes V37, G44, L45, W47)
cvhp29   IGHV1S20     AJ245177 VH germline gene (encodes V37, G44, L45, W47)
cvhp30   IGHV1S21     AJ245178 VH germline gene (encodes V37, G44, L45, W47)
cvhp35   IGHV1S22   IGHV1S22*01 AJ245183 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp36
cvhp36   IGHV1S22   IGHV1S22*02 AJ245184 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp35
cvhp37   IGHV1S23     AJ245185 VH germline gene (encodes V37, G44, L45, W47)
cvhp38   IGHV1S24     AJ245186 VH germline gene (encodes V37, G44, L45, W47)
cvhp39   IGHV1S25     IGHV1S25*01 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp40
cvhp40   IGHV1S25     IGHV1S25*02 VH germline gene (encodes V37, G44, L45, W47)

Putative allele of cvhp39
cvhp41   IGHV1S26     AJ245189 VH germline gene (encodes V37, G44, L45, W47)
cvhp43   IGHV1S27     AJ245191 VH germline gene (encodes V37, G44, L45, W47)
cvhp44   IGHV1S28     AJ245192 VH germline gene (encodes V37, G44, L45, W47)
cvhp45   IGHV1S29     AJ245193 VH germline gene (encodes V37, G44, L45, W47)
cvhp46   IGHV1S30     AJ245194 VH germline gene (encodes V37, G44, L45, W47)
cvhp47   IGHV1S31     AJ245195 VH germline gene (encodes V37, G44, L45, W47)
cvhp31   IGHV1S32     AJ245179 VH germline gene (encodes V37, G44, L45, W47)
cvhp32   IGHV1S33     AJ245180 VH germline gene (encodes V37, G44, L45, W47)
cvhp34   IGHV1S34     AJ245182 VH germline gene (encodes V37, G44, L45, W47)
cvhp42   IGHV1S35     AJ245190 VH germline gene (encodes V37, G44, L45, W47)
cvhp48   IGHV1S36     AJ245196 VH germline gene (encodes V37, G44, L45, W47)
cvhp49   IGHV1S37     AJ245197 VH germline gene (encodes V37, G44, L45, W47)
cvhp33   IGHV1S38     AJ245181 VH germline gene (encodes V37, G44, L45, W47)
cvhp50   IGHV1S39     AJ245198 VH germline gene (encodes V37, G44, L45, W47)
cvhp51   IGHV1S40P     AJ245199 Pseudogene VH (stopcodon at nucleotides 79-81)
cvhp52 IGHV1S41P AJ245200 Pseudogene VH (deletion of one nucleotide at pos. 267)
cvhhp42   IGHV1S42     AJ245148 VHH germline gene (encodes V37, E44, R45, G47)
cvhhp09   IGHV1S43     AJ245115 VHH germline gene (encodes F37, E44, R45, G47) & C33
cvhhp12   IGHV1S44     AJ245118 VHH germline gene (encodes F37, E44, R45, G47) & C33
cvhhp08   IGHV1S45     AJ245114 VHH germline gene (encodes F37, E44, R45, G47) & C33
cvhhp10   IGHV1S46     AJ245116 VHH germline gene (encodes F37, E44, R45, G47) & C33
cvhhp11   IGHV1S47     AJ245117 VHH germline gene (encodes F37, E44, R45, G47) & C33
cvhhp29   IGHV1S48     AJ245135 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp31   IGHV1S49     AJ245137 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp28   IGHV1S50     AJ245134 VHH germline gene (encodes F37, E44, R45, L47) & C33
cvhhp06   IGHV1S51     AJ245112 VHH germline gene (encodes Y37, E44, R45, F47) & C30
cvhhp07   IGHV1S52     AJ245113 VHH germline gene (encodes Y37, E44, R45, F47) & C30
cvhhp16   IGHV1S53   IGHV1S53*01 AJ245122 VHH germline gene (encodes Y37, E44, R45, L47) & C32

Putative allele of cvhhp17
cvhhp17   IGHV1S53   IGHV1S53*02 AJ245123 VHH germline gene (encodes Y37, E44, R45, L47) & C32

Putative allele of cvhp16
cvhhp18   IGHV1S54   IGHV1S54*01 AJ245124 VHH germline gene (encodes Y37, E44, R45, L47) & C32

Putative allele of cvhhp19
cvhhp19   IGHV1S54   IGHV1S54*02 AJ245125 VHH germline gene (encodes Y37, E44, R45, L47) & C32

Putative allele of cvhp18
cvhhp20   IGHV1S55     AJ245126 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp21   IGHV1S56     AJ245127 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp22   IGHV1S57     AJ245128 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp26   IGHV1S58     AJ245132 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp27   IGHV1S59     AJ245133 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp13   IGHV1S60     AJ245119 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp14         AJ245120 More than 5 nucleotide difference with clones cvhhp13 and cvhhp15, but encodes an identical VH germline amino acid sequence.
cvhhp15         AJ245121 More than 5 nucleotide difference with clones cvhhp13 and cvhhp15, but encodes an identical VH germline amino acid sequence.
cvhhp23   IGHV1S61     AJ245129 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp24   IGHV1S62     AJ245130 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp25   IGHV1S63     AJ245131 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp32   IGHV1S64     AJ245138 VHH germline gene (encodes Y37, E44, R45, F47) & C32
cvhhp30   IGHV1S65     AJ245136 VHH germline gene (encodes Y37, E44, R45, L47) & C32
cvhhp34   IGHV1S66     AJ245140 VHH germline gene (encodes Y37, Q44, R45, W47) & C32
cvhhp33   IGHV1S67     AJ245139 VHH germline gene (encodes Y37, E44, R45, F47) & C32
cvhhp35   IGHV1S68     AJ245141 VHH germline gene (encodes Y37, Q44, R45, W47) & C32
cvhhp36   IGHV1S69     AJ245142 VHH germline gene (encodes Y37, Q44, R45, W47) & C32
cvhhp37   IGHV1S70     AJ245143 VHH germline gene (encodes Y37, Q44, R45, W47) & C32
cvhhp38   IGHV1S71     AJ245144 VHH germline gene (encodes Y37, Q44, R45, W47) & C32
cvhhp39         AJ245145 More than 5 nucleotide difference with clones cvhhp38 and cvhhp40 and cvhhp41, but encodes an identical VH germline amino acid sequence.
cvhhp40         AJ245146 More than 5 nucleotide difference with clones cvhhp38 and cvhhp39 and cvhhp41, but encodes an identical VH germline amino acid sequence.
cvhhp41         AJ245147 More than 5 nucleotide difference with clones cvhhp38 and cvhhp39 and cvhhp40, but encodes an identical VH germline amino acid sequence
cvhhc1   IGHV1S72     AF000604 VHH germline gene (encodes F37, E44, C45, L47)
cvhhp01   IGHV1S72     AJ245107 VHH germline gene (encodes F37, E44, C45, L47)
cvhhp02         AJ245108 More than 5 nucleotide difference with clones cvhhp01 and cvhhp03, but encodes an identical germline amino acid sequence
cvhhp03         AJ245109 More than 5 nucleotide difference with clones cvhhp01 and cvhhp02, but encodes an identical VH germline amino acid sequence
cvhhp04   IGHV1S73     AJ245110 VHH germline gene (encodes F37, E44, C45, L47)
cvhhp05   IGHV1S74     AJ245111 VHH germline gene (encodes F37, E44, C45, L47)

1) Curators V.K Nguyen and S. Muyldermans  

References

Nguyen V.K., Muyldermans, S., and Hamers, R. 1998.  The specific variable domain of camel heavy-chain antibodies is encoded in the germline. J. Mol. Biol. 275, 413-418.

Nguyen, V.K., Hamers, R., Wyns, L., and Muyldermans, S. 2000. Camel heavy-chain antibodies: diverse germline VHH and specific mechanisms enlarge the antigen-binding reprtoire. EMBO J. 19, 921-930.

P=psuedogene

Lama VH Nomenclature1                [07/23/2002]

Current Description

 Proposed Designation Gene Serological Allele Sequence Allele GenBank Acc. # Major Features and Other Information
VHH1   IGHV1S1     AF305944 VHH germline gene (encodes F37, E44, R45, G47) & C50
VHH2   IGHV1S2     AF305945 VHH germline gene (encodes F37, E44, R45, G47) & C50
VHH3   IGHV1S3     AF305946 VHH germline gene (encodes Y37, E44, R45, L47) & C30
VHH4   IGHV1S4     AF305947 VHH germline gene (encodes Y37, E44, R45, L47)
VHH5   IGHV1S5     AF305948 VHH germline gene (encodes Y37, E44, R45, W47) & C50
VH1   IGHV1S6     AF305949 VH germline gene (encodes V37, G44, L45, W47)
Vpseudo 1   IGHV1S7-P     AF305950 Pseudogene VH (stopcodon at nucleotides 127-129)
Vpseudo 2   IGHV1S8-P     AF305951 Pseudogene VH (stopcodon at nucleotides 307-309)

1) Curators V.K. Nguyen and S. Muyldermans

Reference

Woolven B.P. van der Logt P., and Nicholls P.J., unpublished.

P = pseudogene

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