Miles Pufall Laboratory

  • pufall
  • Research Interests

    My overarching interest is in how proteins are regulated, and in particular that they are not simply turned on or off by single signals, but are modulated in response to multiple inputs to meet the needs of the cell at the time. In my work, I am studying how different signals, such as post-translational modifications, ligands, and association with other biological macromolecules including DNA, change the structure and dynamics of proteins of the glucocorticoid receptor, a steroid activated transcription factor that is one of the primary targets in the treatment of leukemias and lymphomas.

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    Recent Publications

    Watson LC, Kuchenbecker KM, Schiller BJ, Gross JD, Pufall MA, Yamamoto KR. The glucocorticoid receptor dimer interface allosterically transmits sequence-specific DNA signals.Nat Struct Mol Biol. 2013 Jul;20(7):876-83.

    Serafimova IM, Pufall MA, Krishnan S, Duda K, Cohen MS, Maglathlin RL, McFarland JM, Miller RM, Frödin M, Taunton J. Reversible targeting of noncatalytic cysteines with chemically tuned electrophiles. Nat Chem Biol. 2012 Apr 1;8(5):471-6.

    Shipp LE, Lee JV, Yu CY, Pufall M, Zhang P, Scott DK, Wang JC.Transcriptional regulation of human dual specificity protein phosphatase 1 (DUSP1) gene by glucocorticoids.PLoS One. 2010 Oct 29;5(10):e13754.

    Pufall, Miles A., Graves, Barbara J. Ets-1 Flips for New Partner Pax-5. Structure, Vol. 10, 10-14. 2002. Download pdf reprint

    Garvie, Colin W., Pufall, Miles A., Graves, Barbara J., Wolberger, Cynthia. Structural analysis of the autoinhibition of Ets-1 and its role in protein partnerships., J. Biol. Chem., Vol. 277(47), 45529-36., Nov. 22, 2002. Download pdf reprint

    Pufall, Miles A., Graves Barbara J., Autoinhibitory domains: modular effectors of cellular regulation., Annu. Rev. Cell Dev. Biol., Vol. 18, 421-62., 2002. Download pdf reprint

    Lee, Gregory M., Donaldson, Logan W., Pufall, Miles A., Kang, Hyun-Seo., Pot, Isabelle., Graves, Barbara J., McIntosh, Lawrence P., The structural and dynamic basis of Ets-1 DNA binding autoinhibition., J. Biol. Chem., Vol. 280(8), 7088-99., Feb. 25, 2005. Download pdf reprint

    Pufall, Miles A., Lee, Gregory M., Nelson, Mary L., Kang, Hyun-seo., Velyvis, Algirdas, Kay, Lewis E., McIntosh, Lawrence P., Graves, Barbara J., Variable allosteric regulation of Ets-1 DNA binding by a multiply phosphorylated, unstructured region., Science., Vol. 309(5731), 142-145., July 1, 2005. Download pdf reprint

    Pufall, Miles A.*, Lee, Greg M.*, Meeker, Charles A., Kang, Hyun-Seo, Graves, Barbara J., McIntosh Lawrence P., The affinity of Ets-1 for DNA is modulated by phosphorylation through transient interactions of an unstructured region., J. Mol. Biol., Vol. 382(4), 1014-30., Oct. 17, 2008. Download pdf reprint

    Pufall, Miles A.*, Meijsing, Sebastiaan H.*, So, Alex Y., Bates, Darren L., Chen, Lin., Yamamoto, Keith R., DNA-binding site sequence directs glucocorticoid receptor structure and activity., Science., Vol. 324(5925), 407-410., April 17, 2009. Download pdf reprintreprint

    * Authors contributed equally to the work.

    Affiliations