Collection Medium:

Minimum:

5 mL blood in lavender or yellow top, 1 mL bone marrow aspirate in 
lavender or yellow top, or 3 cu mm tissue.  Tissue may be fresh frozen 
or paraffin embedded formalin fixed.  Body fluids in purple or yellow 
top tubes, Lymph node aspirates in RPMI.

Testing
Schedule:

Weekly

Analytic Time:

7 working days

Interpretive Data:

Used as an adjunct in initial diagnosis or as a monitor of minimal
residual disease.  When used as a monitor of minimal residual disease,
current samples must be analyzed in parallel with a previous diagnostic
sample.

Comments:

Transport fresh tissue on dry ice.  Blood, bone marrow and lymph 
aspirates should be transported on ice.

Test
Limitations:

Specimens fixed in BS fixative are unsuitable for testing.

Methodology:

Multiplex PCR followed by Fluorescence Capillary Electrophoresis

Sample
Processing:

Label transport tube with two patient identifiers, date and time of 
collection.
Relevant clinical information must be submitted with specimen in order 
to provide correct interpretation of test results.
Specimen source is required on requisition for processing.
Tissue specimen should be frozen or processed as a formalin fixed 
paraffin embedded tissue.
Lavender tube is sent as whole blood, no processing necessary.

Sample
Storage:

Blood/Bone Marrow - Room temperature or ambient storage.
Tissue Specimen - Freeze immediately and store frozen or send 
Formalin-fixed paraffin embedded blocks.

Transport
Instructions:

Blood - Do not freeze, ship at ambient temperature.  Ship express mail 
with overnight delivery.
Frozen Tissue - Ship frozen on dry ice.  Ship express mail with 
overnight delivery.  Formalin-fixed paraffin embedded blocks ship at 
ambient temperature.

CPT Code:

83890, 83900, 83901(x2), 83909(x4), 83912; if paraffin block or fresh 
tissue is submitted, remove CPT code 83890 and add replace it with 
83907.