Specimen:

Various

Collection Medium:

Minimum:

5 ml blood in a lavender top or yellow top, 1 ml bone marrow aspirate 
in a lavender top or yellow top,  3 cu mm of fresh frozen tissue or 
Formalin-fixed, paraffin embedded tissue, body fluids in a lavender top 
or yellow top or lymph node aspirates in RPMI.

Testing
Schedule:

Weekly

Analytic Time:

7 working days

Reference Range:

None

Comments:

DNA extracted from blood, bone marrow mononuclear cells or tissue is 
examined for rearrangement of T-cell receptor gamma (TCRG) genes.

Test
Limitations:

Specimens fixed in B5 fixative are unsuitable for testing.

Methodology:

Multiplex Polymerase Chain Reaction (PCR) followed by Fluorescence 
Capillary Electrophoresis

Sample
Processing:

Label transport tube with two patient identifiers, date and time of 
collection.
Relevant clinical information must be submitted with specimen in order 
to provide correct interpretation of test results.
Specimen source is required on requisition for processing.
Tissue specimen should be frozen.
Lavender tube is sent as whole blood, no processing necessary.

Sample
Storage:

Blood/Bone Marrow - Room temperature or ambient storage.
Tissue Specimen - Freeze immediately and store frozen or send 
Formalin-fixed paraffin embedded blocks.

Transport
Instructions:

Blood - Do not freeze, ship at ambient temperature.  Ship express mail 
with overnight delivery.
Frozen Tissue - Ship frozen on dry ice.  Ship express mail with 
overnight delivery.  Formalin-fixed paraffin embedded blocks ship at 
ambient temperature.

CPT Code:

83890, 83900, 83901, 83909 (x3), 83912
If paraffin block or fresh tissue is submitted, remove CPT code 83890 
and add 83907.